Seasonal variation in wild pig (Sus scrofa) diet revealed by DNA metabarcoding
Wildlife Society Bulletin, EarlyView.Using DNA metabarcoding of fecal samples, we investigated the diet of wild pigs at an extensive bottomland hardwood forest ecosystem within Felsenthal National Wildlife Refuge in Southern Arkansas. We found that wild pig diet was highly diverse and included at least 74 plant families and 106 genera and 23 species of vertebrates.
Kenneth C. Wilson +3 more
wiley +1 more source
The Journal of Dermatology, EarlyView.ABSTRACT Atopic dermatitis (AD) essentially exhibits dysbiosis of skin fungal microbiome, mycobiome, characterized by depletion of Malassezia. The effects of recent systemic therapies for AD on skin mycobiome were not understood enough. We examined changes of skin mycobiome before and after systemic treatments with anti‐IL‐4Rα antibody (dupilumab: DUP)
Yuta Koike +5 more
wiley +1 more source
Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three Lycium Barbarum Producing Regions of China. [PDF]
Microbiol Spectr, 2022 Chen K +7 more
europepmc +1 more source
Dietary resilience of coral reef fishes to habitat degradation
Journal of Animal Ecology, EarlyView.Metabarcoding of gut contents shows that two common benthic‐feeding reef fishes with different feeding stratgies—a butterflyfish (Chaetodon capistratus) and a hamlet (Hypoplectrus puella)—shift diets on degraded reefs. These shifts mirror contrasting patterns in body condition: butterflyfish showed strong individual variation, whereas condition was ...
Friederike Clever +9 more
wiley +1 more source
An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms. [PDF]
STAR Protoc, 2021 Bhoyar RC +7 more
europepmc +1 more source
Is the endophyte‐based plant protection against aphids mediated by changes in the insect microbiome?
Insect Science, EarlyView.We showed that the fungal endophyte Epichloë strain AR37 increased the plant resistance against aphids via the production of indole diterpene alkaloids. The reduction in aphid performance was not associated with changes in abundance/composition/diversity of the insect's bacterial microbiota, but additional endophyte effects on this microbiota cannot be
Daniel A. Bastías +5 more
wiley +1 more source
Rumen Epithelial Communities Share a Core Bacterial Microbiota: A Meta-Analysis of 16S rRNA Gene Illumina MiSeq Sequencing Datasets. [PDF]
Front Microbiol, 2021 Anderson CJ +2 more
europepmc +1 more source
Blubber Thickening Driven by UCP1 Inactivation: Insights from a Cetacean‐Like Transgenic Mouse Model
Integrative Zoology, EarlyView.UCP1 inactivation of cetaceans in mice drives BAT whitening and iWAT hyperplasia, promoting fat accumulation for aquatic adaptation. Abstract Cetaceans possess thick blubber, a specialized adipose tissue essential for thermal insulation, a streamlined body form, energy storage, and buoyancy. However, the mechanisms that underpin this adaptation are not
Qian Zhang +5 more
wiley +1 more source
Integrative Zoology, EarlyView.Avian haemosporidian blood parasites are typically identified through Sanger sequencing of a partial cytochrome b fragment, the MalAvi barcoding region. Next‐generation sequencing is seldom used for avian blood parasite identification; this study demonstrates a higher detection rate of co‐infections via metabarcoding and its possible implications ...
Peter Pibaque +9 more
wiley +1 more source
Long‐read sequencing for biodiversity analyses—A comprehensive guide
Methods in Ecology and Evolution, EarlyView.Abstract DNA‐based monitoring of biodiversity has revolutionised our ability to describe communities and rapidly assess anthropogenic impacts on biodiversity. Currently established molecular methods for biomonitoring rely heavily on classic metabarcoding utilising short reads, mostly through Illumina data.
Iliana Bista, Alexandra Lino
wiley +1 more source