Results 71 to 80 of about 339,340 (383)

The identification of histidine ligands to cytochrome a in cytochrome c oxidase [PDF]

, 1985
A histidine auxotroph of Saccharomyces cerevisiae has been used to metabolically incorporate [1,3-15N2] histidine into yeast cytochrome c oxidase. Electron nuclear double resonance (ENDOR) spectroscopy of cytochrome a in the [15N]histidine-substituted ...
Chan, Sunney I., Martin, Craig T., Scholes, Charles P.   +2 more
core  

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Crystal structure and Hirshfeld surface analysis of 4-[4-(1H-benzo[d]imidazol-2-yl)phenoxy]phthalonitrile dimethyl sulfoxide monosolvate

Acta Crystallographica Section E: Crystallographic Communications, 2019
This work presents the synthesis and structural characterization of [4-(1H-benzo[d]imidazol-2-yl)phenoxy]phthalonitrile, a phthalonitrile derivative carrying a benzimidazole moiety.
Sibel Demir Kanmazalp, Pınar Şen, Necmi Dege, Salih Zeki Yildiz, Namık Ozdemir, Irina A. Golenya   +5 more
doaj   +1 more source

LC3 and STRAP regulate actin filament assembly by JMY during autophagosome formation. [PDF]

, 2019
During autophagy, actin filament networks move and remodel cellular membranes to form autophagosomes that enclose and metabolize cytoplasmic contents. Two actin regulators, WHAMM and JMY, participate in autophagosome formation, but the signals linking ...
Axe, Cooper, Coutts, Coutts, Coyle, Dayel, Demonacos, Firat-Karalar, Graef, Hu, Jahreiss, Kaiser, Kast, Klionsky, Koronakis, Li, Mathiowetz, Mi, Okrut, Padrick, Padrick, Palmgren, Pankiv, Pankiv, Papinski, Quinlan, Quinlan, R. Dyche Mullins, Ran, Rohatgi, Rohatgi, Schlüter, Shibutani, Shikama, Sun, Urbanek, Welch, Xiaohua Hu, Yamamoto, Zhao, Zuchero   +40 more
core   +2 more sources

Studien über Imidazole [PDF]

Justus Liebigs Annalen der Chemie, 1893
n ...
openaire   +2 more sources

Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity

FEBS Open Bio, EarlyView.
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo, Natalia Cuervo, Carmen García‐Martín, Andrés López‐Perrote, Clara Reglero, Adrián Maqueda‐Real, Ana González‐Corpas, Marina Serna, Diego Megías, Alejo Efeyan, Solip Park, Oscar Llorca   +11 more
wiley   +1 more source

Crystal structure of catena-poly[[bis(acetato-κO)copper(II)]-bis[μ-4-(1H-imidazol-1-yl)phenol]-κ2N3:O;κ2O:N3]

Acta Crystallographica Section E: Crystallographic Communications, 2017
In the title compound, [Cu(CH3COO)2(C9H8N2O)2]n, the CuII ion resides on a centre of inversion, displaying a tetragonally distorted octahedral coordination environment defined by two pairs of N and O atoms of symmetry-related 4-(1H-imidazol-1-yl)phenol ...
Mehmet Poyraz, Musa Sarı
doaj   +1 more source

Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography [PDF]

, 2017
In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine
Cornely, Dr. Kathleen, Foley, Caroline
core   +1 more source

Targeting RAGE with Nanobodies for Molecular Imaging of Cancers and Alzheimer's Disease

Advanced Biology, EarlyView.
RAGE‐specific nanobodies were isolated via phage display and characterized by ELISA, cell ELISA, and SPR. In vivo imaging in renal carcinoma and Alzheimer's disease mouse models demonstrated that NbF8, the highest‐affinity clone, selectively targeted RAGE‐overexpressing tumors and brain tissues, highlighting its potential as a molecular imaging agent ...
Guangfeng Liang, Fujing Wang, Wei Xiong, Guangwei Shi, Junling Yuan, Yang Li, Hongyan Zhang, Yanmei Xing, Shan Jin, Kongjun Yang, Zhongliang Dai, Jichao Sun, Zhijie Li, Jianhong Wang   +13 more
wiley   +1 more source

Molecular Recognition of Imidazole-Based Drug Molecules by Cobalt(III)- and Zinc(II)-Coproporphyrins in Aqueous Media

Molecules, 2023
The methods of 1H NMR, spectrophotometric titration, mass spectrometry and elemental analysis are applied to determine the selective binding ability of Co(III)- and Zn(II)-coproporphyrins I towards a series of imidazole-based drug molecules with a wide ...
Galina Mamardashvili, Elena Kaigorodova, Ivan Lebedev, Nugzar Mamardashvili   +3 more
doaj   +1 more source