Results 51 to 60 of about 321,783 (122)

A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material

Bio-Protocol, 2019
We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from single
khaled amara, Lena Israelsson, Ragnhild Stålesen, Peter Sahlström, Johanna Steen, Vivianne Malmström, Caroline Grönwall   +6 more
doaj   +1 more source

Data on the nucleotide composition of the first codons encoding the complementary determining region 3 (CDR3) in immunoglobulin heavy chains

Data in Brief, 2018
The highly variable complementary determining region 3 (CDR3) of antibodies is generated through recombination of immunoglobulin heavy chain variable (IGHV), diversity, and joining genes.
Linnea Thörnqvist, Mats Ohlin
doaj  

MAK33 antibody light chain amyloid fibrils are similar to oligomeric precursors. [PDF]

PLoS ONE, 2017
Little structural information is available so far on amyloid fibrils consisting of immunoglobulin light chains. It is not understood which features of the primary sequence of the protein result in fibril formation.
Manuel Hora, Riddhiman Sarkar, Vanessa Morris, Kai Xue, Elke Prade, Emma Harding, Johannes Buchner, Bernd Reif   +7 more
doaj   +1 more source

A diverse repertoire of human immunoglobulin variable genes in a chicken B cell line is generated by both gene conversion and somatic hypermutation

Frontiers in Immunology, 2015
Chicken immune responses to human proteins are often more robust than rodent responses because of the phylogenetic relationship between the different species. For discovery of a diverse panel of unique therapeutic antibody candidates, chickens therefore
Philip A. Leighton, Benjamin eSchusser, Henry eYi, Jacob eGlanville, William eHarriman   +4 more
doaj   +1 more source

A genome-wide association study for allergen component sensitizations identifies allergen component–specific and allergen protein group–specific associations

Journal of Allergy and Clinical Immunology: Global, 2023
Background: Allergic diseases are some of the most common diseases worldwide. Genome-wide association studies (GWASs) have been conducted to elucidate the genetic factors of allergic diseases.
Wataru Morii, PhD, Koki Kasai, BS, Takako Nakamura, MSc, Daisuke Hayashi, MD, Monami Hara, MD, Tatsuhiko Naito, MD, PhD, Kyuto Sonehara, MD, PhD, Tatsuki Fukuie, MD, PhD, Mayako Saito-Abe, MD, PhD, Limin Yang, MD, PhD, Kiwako Yamamoto-Hanada, MD, PhD, Masami Narita, MD, PhD, Kazushi Maruo, PhD, Yukinori Okada, MD, PhD, Emiko Noguchi, MD, PhD, Yukihiro Ohya, MD, PhD   +15 more
doaj  

The zipper mechanism in phagocytosis: energetic requirements and variability in phagocytic cup shape [PDF]

arXiv, 2010
Phagocytosis is the fundamental cellular process by which eukaryotic cells bind and engulf particles by their cell membrane. Particle engulfment involves particle recognition by cell-surface receptors, signaling and remodeling of the actin cytoskeleton to guide the membrane around the particle in a zipper-like fashion. Despite the signaling complexity,
arxiv  

Holographic immunoassays [PDF]

arXiv, 2020
The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM)
arxiv  

Crowding-Regulated Binding of Divalent Biomolecules [PDF]

Phys. Rev. Lett. 130, 258401 (2023)
Macromolecular crowding affects biophysical processes as diverse as diffusion, gene expression, cell growth, and senescence. Yet, there is no comprehensive understanding of how crowding affects reactions, particularly multivalent binding. Herein, we use scaled particle theory and develop a molecular simulation method to investigate the binding of ...
arxiv   +1 more source

Somatic Mutation in Immunoglobulin Gene Variable Region in Patients with Chronic Lymphocytic Leukemia and Prognostic Impact

Basic & Clinical Cancer Research, 2017
Chronic lymphocyte leukemia (CLL) is the most common leukemia in elderly individuals that is accompanied by the presence of CD5/CD19/CD20/CD23 positive, FMC7 negative and reduced levels of surface membrane Immunoglobuline (IgM & IgD), CD79b on B ...
Mehrnoosh Pashaei, Mahdieh Shokrollahi, Parviz Kokhaei   +2 more
doaj  

Attractors in residual interactions explain the differentially-conserved stability of Immunoglobulins [PDF]

arXiv, 2014
Proteins belonging to immunoglobulin superfamily(IgSF) show remarkably conserved nature both in their folded structure and in their folding process, but they neither originate from very similar sequences nor demonstrate functional conservation. Treating proteins as fractal objects, without studying spatial conservation in positioning of particular ...
arxiv