Substantially increased sensitivity of the spot-ELISA for the detection of anti-insulin antibody-secreting cells using a capture antibody and enzyme-conjugated insulin [PDF]
, 1990 This paper describes an antibody capture spot-ELISA for the detection of anti-insulin antibody-secreting cells. The assay is based on the binding of secreted antibodies by immobilised isotype-specific capture antibodies and subsequent detection of ...
Benner, R. (Robbert) +2 more
core +1 more source
The Cell Physiology of Biphasic Insulin Secretion
Physiology, 2000 Glucose-stimulated insulin secretion consists of a transient first phase followed by a sustained second phase. Diabetes (type II) is associated with abnormalities in this release pattern. Here we review the evidence that biphasic insulin secretion reflects exocytosis of two functional subsets of secretory granules and the implications for diabetes.
Rorsman, P +5 more
openaire +4 more sources
Norepinephrine Inhibits Endocytosis In Insulin-secreting Cells [PDF]
Biophysical Journal, 2009 Norepinephrine (NE) is a well-known physiological inhibitor of insulin secretion in pancreatic β cells. We investigated modulation of exocytosis and endocytosis by NE in INS 832/13 cells using whole-cell capacitance measurements. Exocytosis was stimulated by depolarizing pulses from −70mV to +10mV of variable duration and was followed by compensatory ...
Lindau Manfred +4 more
openaire +2 more sources
Genetic obesity increases pancreatic expression of mitochondrial proteins which regulate cholesterol efflux in BRIN-BD11 insulinoma cells [PDF]
, 2019 Pancreatic β-cells are sensitive to fluctuations in cholesterol content, which can damage the insulin secretion pathway, contributing to the aetiology of type 2 diabetes mellitus.
Caridis, Anna Maria +4 more
core +1 more source
In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion. [PDF]
, 2006 International audienceInsulin-secreting pancreatic beta cells are exceptionally rich in zinc. In these cells, zinc is required for zinc-insulin crystallization within secretory vesicles.
Chimienti, Fabrice +10 more
core +3 more sources
Characterization of prenylcysteine methyltransferase in insulin-secreting cells [PDF]
Biochemical Journal, 1996 Prenylcysteine carboxymethyltransferase, an enzyme involved in the post-translational modification of many signalling proteins, was characterized in insulin-secreting INS-1 cells and normal rat pancreatic islets. The activity of this enzyme was monitored by the methylation of an artificial substrate (a prenylated cysteine analogue) with S-adenosyl ...
Guodong Li +2 more
openaire +3 more sources
Cyclic AMP signalling in pancreatic islets [PDF]
, 2010 Cyclic 3'5'AMP (cAMP) is an important physiological amplifier of glucose-induced insulin secretion by the pancreatic islet β-cell, where it is formed by the activity of adenylyl cyclases, which are stimulated by glucose, through elevation in ...
Furman, Brian +2 more
core +1 more source
Design Principles of Pancreatic Islets: Glucose-dependent Coordination of Hormone Pulses [PDF]
, 2015 Pancreatic islets are functional units involved in glucose homeostasis. The multicellular system comprises three main cell types; $\beta$ and $\alpha$ cells reciprocally decrease and increase blood glucose by producing insulin and glucagon pulses, while ...
Hara, Manami +2 more
core +12 more sources
β-Cell Replacement Strategies: The Increasing Need for a “β-Cell Dogma”
Frontiers in Genetics, 2017 Type 1 diabetes is an auto-immune disease resulting in the loss of pancreatic β-cells and, consequently, in chronic hyperglycemia. Insulin supplementation allows diabetic patients to control their glycaemia quite efficiently, but treated patients still ...
Andhira Vieira +6 more
doaj +1 more source
Generation of human islet cell type-specific identity genesets
Nature Communications, 2022 Generation of surrogate cells with stable functional identities is crucial for developing cell-based therapies. Efforts to produce insulin-secreting replacement cells to treat diabetes require reliable tools to assess islet cellular identity.
Léon van Gurp +9 more
doaj +1 more source