Results 31 to 40 of about 57,117 (262)

Effect of 2‐mercaptoethanol on glutathione levels, cystine uptake and insulin secretion in insulin‐secreting cells [PDF]

European Journal of Biochemistry, 1992
The role of glutathione (GSH) in the differentiated state of insulin‐secreting cells was studied using 2‐mercaptoethanol as a means of varying intracellular GSH levels. 2‐Mercaptoethanol (50 μM) caused a marked increase of GSH in two rat insulinoma cell lines, RINm5F and INS‐1, the latter being dependent on the presence of 2‐mercaptoethanol for ...
Janjic D, Wollheim CB
openaire   +3 more sources

The AE804 and AE806 antibodies label mouse insulin-secreting beta cells by immunofluorescence in histological frozen sections

Antibody Reports, 2019
The AE804 and AE806 antibodies detect the insulin-secreting beta cells by immunofluorescence in mice pancreatic islets.
Marta Perez Frances, Fabrizio Thorel
doaj   +1 more source

Disruption of Glucose Sensing and Insulin Secretion by Ribozyme Kir6.2-Gene Targeting in Insulin-Secreting Cells [PDF]

Endocrinology, 2004
The ATP-sensitive K+ (KATP) channel, composed of Kir6.2 and sulfonylurea receptor (SUR1), in pancreatic beta-cells is believed to serve as a metabolic sensor regulating insulin secretion according to glucose levels. Thus, genetic disruption of Kir6.2 expression may impair KATP channel function in glucose sensing and insulin secretion.
Li, Li, Asheebo, Rojas, Jianping, Wu, Chun, Jiang   +3 more
openaire   +2 more sources

A Humanized Mouse Strain That Develops Spontaneously Immune-Mediated Diabetes

Frontiers in Immunology, 2021
To circumvent the limitations of available preclinical models for the study of type 1 diabetes (T1D), we developed a new humanized model, the YES-RIP-hB7.1 mouse.
Sandrine Luce, Sandrine Luce, Sophie Guinoiseau, Sophie Guinoiseau, Alexis Gadault, Alexis Gadault, Franck Letourneur, Patrick Nitschke, Marc Bras, Michel Vidaud, Pierre Charneau, Etienne Larger, Etienne Larger, Maikel L. Colli, Decio L. Eizirik, Decio L. Eizirik, François Lemonnier, François Lemonnier, Christian Boitard, Christian Boitard, Christian Boitard   +20 more
doaj   +1 more source

Effects of sulphonylureas and diazoxide on insulin secretion and nucleotide‐sensitive channels in an insulin‐secreting cell line [PDF]

British Journal of Pharmacology, 1988
The effects of various sulphonylureas and diazoxide on insulin secretion and the activity of various channels have been studied using tissue culture and patch‐clamp methods in an insulinsecreting cell line derived from a rat islet cell tumour. Tolbutamide, glibenclamide and HB699 increased the rate of insulin release by 2–5 fold. The concentrations of
Sturgess, N. C., Kozlowski, R. Z., Carrington, C. A., Hales, C. N., Ashford, M. L. J.   +4 more
openaire   +3 more sources

Mitochondrial Complexes I and II Are More Susceptible to Autophagy Deficiency in Mouse β-Cells [PDF]

Endocrinology and Metabolism, 2015
BackgroundDamaged mitochondria are removed by autophagy. Therefore, impairment of autophagy induces the accumulation of damaged mitochondria and mitochondrial dysfunction in most mammalian cells.
Min Joo Kim, Ok Kyong Choi, Kyung Sil Chae, Min Kyeong Kim, Jung Hee Kim, Masaaki Komatsu, Keiji Tanaka, Hakmo Lee, Sung Soo Chung, Soo Heon Kwak, Young Min Cho, Kyong Soo Park, Hye Seung Jung   +12 more
doaj   +1 more source

Human islets contain four distinct subtypes of β cells

Nature Communications, 2016
Dysfunction or loss of insulin-secreting β cells in the pancreas is a hallmark of diabetes. Here, Dorrell et al.identify four subpopulations of β cells in humans, which differ in gene expression and insulin secretion kinetics, and the abundance of which ...
Craig Dorrell, Jonathan Schug, Pamela S. Canaday, Holger A. Russ, Branden D. Tarlow, Maria T. Grompe, Tamara Horton, Matthias Hebrok, Philip R. Streeter, Klaus H. Kaestner, Markus Grompe   +10 more
doaj   +1 more source

2-Ketoisocaproate transport in insulin-secreting cells

Bioscience Reports, 1992
The transport of the nutrient secretagogue 2-ketoisocaproate (KIC) was studied in isolated rat pancreatic islets and in the HIT-T15 insulinoma cell line using an oil-filtration technique. In both islets and HIT-T15 cells, KIC uptake was a slow process, not reaching equilibrium within 10 min KIC transport was not dependent upon Na+ in the medium, was ...
L, Best, R, Trebilcock, S, Tomlinson
openaire   +2 more sources

The neuronal K+Cl− co-transporter 2 (Slc12a5) modulates insulin secretion

Scientific Reports, 2017
Intracellular chloride concentration ([Cl−]i) in pancreatic β-cells is kept above electrochemical equilibrium due to the predominant functional presence of Cl− loaders such as the Na+K+2Cl− co-transporter 1 (Slc12a2) over Cl−extruders of unidentified ...
Shams Kursan, Timothy S. McMillen, Pavani Beesetty, Eduardo Dias-Junior, Mohammed M. Almutairi, Abu A. Sajib, J. Ashot Kozak, Lydia Aguilar-Bryan, Mauricio Di Fulvio   +8 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source