Results 51 to 60 of about 90,101 (333)

2-Ketoisocaproate transport in insulin-secreting cells

Bioscience Reports, 1992
The transport of the nutrient secretagogue 2-ketoisocaproate (KIC) was studied in isolated rat pancreatic islets and in the HIT-T15 insulinoma cell line using an oil-filtration technique. In both islets and HIT-T15 cells, KIC uptake was a slow process, not reaching equilibrium within 10 min KIC transport was not dependent upon Na+ in the medium, was ...
S. Tomlinson, Leonard Best, R. Trebilcock   +2 more
openaire   +3 more sources

A role for the extracellular calcium-sensing receptor in cell-cell communication in pancreatic islets of Langerhans [PDF]

, 2008
Background: The extracellular calcium-sensing receptor (CaR) is expressed in many tissues that are not associated with Ca2+ homeostasis, including the endocrine cells in pancreatic islets of Langerhans.
Burns, Christopher J., Jones, Peter M., Kitsou-Mylona, Isidora, Persaud, Shanta J., Squires, Paul E.   +4 more
core   +1 more source

Human islets contain four distinct subtypes of β cells

Nature Communications, 2016
Dysfunction or loss of insulin-secreting β cells in the pancreas is a hallmark of diabetes. Here, Dorrell et al.identify four subpopulations of β cells in humans, which differ in gene expression and insulin secretion kinetics, and the abundance of which ...
Craig Dorrell, Jonathan Schug, Pamela S. Canaday, Holger A. Russ, Branden D. Tarlow, Maria T. Grompe, Tamara Horton, Matthias Hebrok, Philip R. Streeter, Klaus H. Kaestner, Markus Grompe   +10 more
doaj   +1 more source

Effects of sulphonylureas and diazoxide on insulin secretion and nucleotide‐sensitive channels in an insulin‐secreting cell line [PDF]

British Journal of Pharmacology, 1988
The effects of various sulphonylureas and diazoxide on insulin secretion and the activity of various channels have been studied using tissue culture and patch‐clamp methods in an insulinsecreting cell line derived from a rat islet cell tumour. Tolbutamide, glibenclamide and HB699 increased the rate of insulin release by 2–5 fold. The concentrations of
Sturgess, N. C., Kozlowski, R. Z., Carrington, C. A., Hales, C. N., Ashford, M. L. J.   +4 more
openaire   +3 more sources

ABCA12 regulates insulin secretion from β‐cells [PDF]

EMBO reports, 2020
Dysregulation of lipid homeostasis is intimately associated with defects in insulin secretion, a key feature of type 2 diabetes. Here, we explore the role of the putative lipid transporter ABCA12 in regulating insulin secretion from β-cells. Mice with β-cell-specific deletion of Abca12 display impaired glucose-stimulated insulin secretion and eventual ...
Gloria M Ursino, Ying Fu, Denny L Cottle, Nigora Mukhamedova, Lynelle K Jones, Hann Low, Ming Shen Tham, Wan Jun Gan, Natalie A Mellett, Partha P Das, Jacquelyn M Weir, Michael Ditiatkovski, Stacey Fynch, Peter Thorn, Helen E Thomas, Peter J Meikle, Helena C Parkington, Ian M Smyth, Dmitri Sviridov   +18 more
openaire   +4 more sources

Catechol estrogens stimulate insulin secretion in pancreatic β-cells via activation of the transient receptor potential A1 (TRPA1) channel [PDF]

, 2019
Estrogen hormones play an important role in controlling glucose homeostasis and pancreatic β-cell function. Despite the significance of estrogen hormones for regulation of glucose metabolism, little is known about the roles of endogenous estrogen ...
Cabrera, Over, Cerne, Rok, Chen, Xingjuan, Efanov, Alexander M., Ficorilli, James V., Ma, Wenzhen, Obukhov, Alexander G., Syed, Samreen K.   +7 more
core   +1 more source

The neuronal K+Cl− co-transporter 2 (Slc12a5) modulates insulin secretion

Scientific Reports, 2017
Intracellular chloride concentration ([Cl−]i) in pancreatic β-cells is kept above electrochemical equilibrium due to the predominant functional presence of Cl− loaders such as the Na+K+2Cl− co-transporter 1 (Slc12a2) over Cl−extruders of unidentified ...
Shams Kursan, Timothy S. McMillen, Pavani Beesetty, Eduardo Dias-Junior, Mohammed M. Almutairi, Abu A. Sajib, J. Ashot Kozak, Lydia Aguilar-Bryan, Mauricio Di Fulvio   +8 more
doaj   +1 more source

Effect of 2‐mercaptoethanol on glutathione levels, cystine uptake and insulin secretion in insulin‐secreting cells [PDF]

European Journal of Biochemistry, 1992
The role of glutathione (GSH) in the differentiated state of insulin‐secreting cells was studied using 2‐mercaptoethanol as a means of varying intracellular GSH levels. 2‐Mercaptoethanol (50 μM) caused a marked increase of GSH in two rat insulinoma cell lines, RINm5F and INS‐1, the latter being dependent on the presence of 2‐mercaptoethanol for ...
Danilo Janjic, Claes B. Wollheim
openaire   +3 more sources

Insulin secretion assays in an engineered MIN6 cell line

MethodsX, 2023
Insulin secretion from pancreatic beta cells is crucial for maintaining glucose homeostasis. The murine insulinoma derived MIN6 cell line is commonly used as a model for insulin secretion studies. However, its glucose responsiveness wanes with passaging, and insulin secretion is traditionally measured by expensive and time-consuming RIA or ELISA.
Liu Yang, Wenbiao Chen
openaire   +3 more sources

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source