Results 11 to 20 of about 121,748 (297)

Fresh Islets are more Effective for Islet Transplantation than Cultured Islets [PDF]

Cell Transplantation, 2012
For clinical islet transplantation, isolated islets deteriorate rapidly in culture, although culturing islets prior to transplantation provides flexibility for evaluation of isolated islets and pretreatment of patients. In the present study, we compared human fresh islets to cultured islets with in vitro and in vivo assays.
Takeshi Itoh, Nicholas Onaca, Shinichi Matsumoto, Tetsuya Ikemoto, Daisuke Chujo, Bashoo Naziruddin, H. Peng, Morihito Takita, Andrew Jackson, Hirofumi Noguchi, Hirofumi Noguchi, Naoya Kobayashi, Yasutaka Fujita, Marlon F. Levy, Masayuki Shimoda, Koji Sugimoto   +15 more
openaire   +3 more sources

Superiority of Small Islets in Human Islet Transplantation [PDF]

Diabetes, 2007
Many factors influence the outcome of islet transplantation. As islets in the early posttransplant setting are supplied with oxygen by diffusion only and are in a hypoxic state in the portal system, we tested whether small human islets are superior to large islets both in vitro and in vivo.
Lehmann, R, Zuellig, R A, Kugelmeier, P, Baenninger, P B, Moritz, W, Perren, A, Clavien, P A, Weber, M, Spinas, G A   +8 more
openaire   +3 more sources

Islet Gene View—a tool to facilitate islet research

Life Science Alliance, 2022
Characterization of gene expression in pancreatic islets and its alteration in type 2 diabetes (T2D) are vital in understanding islet function and T2D pathogenesis. We leveraged RNA sequencing and genome-wide genotyping in islets from 188 donors to create the Islet Gene View (IGW) platform to make this information easily accessible to the scientific ...
Olof Asplund, Petter Storm, Vikash Chandra, Gad Hatem, Emilia Ottosson-Laakso, Dina Mansour-Aly, Ulrika Krus, Hazem Ibrahim, Emma Ahlqvist, Tiinamaija Tuomi, Erik Renström, Olle Korsgren, Nils Wierup, Mark Ibberson, Michele Solimena, Piero Marchetti, Claes Wollheim, Isabella Artner, Hindrik Mulder, Ola Hansson, Timo Otonkoski, Leif Groop, Rashmi B Prasad   +22 more
openaire   +7 more sources

Islet Culture/Preservation before Islet Transplantation [PDF]

Cell Medicine, 2015
Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture ...
Yasufumi Noguchi, Masayuki Matsushita, Naoya Kobayashi, Kiyoto Kurima, Issei Saitoh, Chika Miyagi-Shiohira, Masami Watanabe, Hirofumi Noguchi   +7 more
openaire   +2 more sources

Islet Biology and Metabolism [PDF]

Metabolites, 2021
This Special Issue, Islet Biology and Metabolism, was intended as a collection of studies highlighting the importance of the pancreatic islet—in both form and function—to our growing understanding of metabolic physiology and disease [...]
Belinda Yau, Melkam A. Kebede
openaire   +4 more sources

Inducible Mosaic Cell Labeling Provides Insights Into Pancreatic Islet Morphogenesis

Frontiers in Cell and Developmental Biology, 2020
Pancreatic islets, discrete microorgans embedded within the exocrine pancreas, contain beta cells which are critical for glucose homeostasis. Loss or dysfunction of beta cells leads to diabetes, a disease with expanding global prevalence, and for which ...
Julia Freudenblum, Dirk Meyer, Robin A. Kimmel   +2 more
doaj   +1 more source

Pancreatic β-Cell O-GlcNAc Transferase Overexpression Increases Susceptibility to Metabolic Stressors in Female Mice

Cells, 2021
The nutrient-sensor O-GlcNAc transferase (Ogt), the sole enzyme that adds an O-GlcNAc-modification onto proteins, plays a critical role for pancreatic β-cell survival and insulin secretion.
Ramkumar Mohan, Seokwon Jo, Elina Da Sol Chung, Eunice Oribamise, Amber Lockridge, Juan E. Abrahante-Lloréns, Hai-Bin Ruan, Xiao-Yong Yang, Emilyn U. Alejandro   +8 more
doaj   +1 more source

Glucolipotoxic Stress-Induced Mig6 Desensitizes EGFR Signaling and Promotes Pancreatic Beta Cell Death

Metabolites, 2023
A loss of functional beta cell mass is a final etiological event in the development of frank type 2 diabetes (T2D). To preserve or expand beta cells and therefore treat/prevent T2D, growth factors have been considered therapeutically but have largely ...
Yi-Chun Chen, Andrew J. Lutkewitte, Halesha D. Basavarajappa, Patrick T. Fueger   +3 more
doaj   +1 more source

Methods of Human Islet Culture for Transplantation

Cell Transplantation, 2004
The ability to maintain isolated human islet preparations in tissue culture has recently been adopted by most islet transplant centers, and improves the safety as well as the practicality of islet transplantation. Maintaining islet viability and recovery,
T. B. Murdoch, D. McGhee-Wilson, A. M. J. Shapiro, J. R. T. Lakey Ph.D.   +3 more
doaj   +1 more source

Novel regulators of islet function identified from genetic variation in mouse islet Ca2+ oscillations

eLife, 2023
Insufficient insulin secretion to meet metabolic demand results in diabetes. The intracellular flux of Ca2+ into β-cells triggers insulin release. Since genetics strongly influences variation in islet secretory responses, we surveyed islet Ca2+ dynamics ...
Christopher H Emfinger, Lauren E Clark, Brian Yandell, Kathryn L Schueler, Shane P Simonett, Donnie S Stapleton, Kelly A Mitok, Matthew J Merrins, Mark P Keller, Alan D Attie   +9 more
doaj   +1 more source