Results 21 to 30 of about 91,246 (148)

Islet assessment for transplantation [PDF]

Current Opinion in Organ Transplantation, 2009
There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation. Development, testing, and validation of such assays have been the subject of intense investigation for the last decade. These efforts are reviewed, highlighting the most recent results while
Papas, Klearchos K., Suszynski, Thomas M., Colton, Clark K.   +2 more
openaire   +4 more sources

Comparison of Cooling Systems during Islet Purification

Cell Transplantation, 2006
Islet isolation is a complex procedure that includes digestion and purification of pancreatic tissue. As we move towards clinical regulatory control and standardization, understanding of the detailed stages of the procedure have become increasingly ...
Sue Swift, Tatsuya Kin, Mohammadreza Mirbolooki, Richard Wilson, Jonathan R. T. Lakey Ph.D.   +4 more
doaj   +1 more source

Evaluating Islet Cell Isolation and Transplantation From Donors Following Medical Assistance in Dying

Transplantation Direct
Background. Limited information is available regarding outcomes of islet cell isolation (ICI) and transplantation (ITx) using medical assistance in dying (MAiD) donors.
Alessandro Parente, MD, PhD, Kevin Verhoeff, MD, PhD, Tatsuya Kin, MD, PhD, Joshua Hefler, MD, PhD, Braulio A. Marfil-Garza, MD, PhD, Norberto Sanchez-Fernandez, MD, Anna Lam, MD, MSc, James Lyon, MD, PhD, Doug O’Gorman, BSc, Khaled Z. Dajani, MD, Blaire L. Anderson, MD, David L. Bigam, MD, MSc, Patrick E. MacDonald, MD, PhD, A. M. James Shapiro, MD, PhD, FRCS (Eng), FRCSC, MSM   +13 more
doaj   +1 more source

Detection of Microbial Contamination during Human Islet Isolation

Cell Transplantation, 2007
Current good manufacturing practice (cGMP) islet processing facilities provide an ultraclean environment for the safe production of clinical grade islets for transplantation into immunosuppressed diabetic recipients.
Tatsuya Kin, Shawn Rosichuk, A. M. James Shapiro, Jonathan R. T. Lakey Ph.D.   +3 more
doaj   +1 more source

Estimation of Pancreas Weight from Donor Variables

Cell Transplantation, 2006
Previous studies have identified several donor factors affecting the outcome of islet isolation. Pancreas weight has not been considered as a donor selection criterion, because a value cannot be obtained prior to organ procurement.
Tatsuya Kin, Travis B. Murdoch, A. M. James Shapiro, Jonathan R. T. Lakey Ph.D.   +3 more
doaj   +1 more source

Inducible Mosaic Cell Labeling Provides Insights Into Pancreatic Islet Morphogenesis

Frontiers in Cell and Developmental Biology, 2020
Pancreatic islets, discrete microorgans embedded within the exocrine pancreas, contain beta cells which are critical for glucose homeostasis. Loss or dysfunction of beta cells leads to diabetes, a disease with expanding global prevalence, and for which ...
Julia Freudenblum, Dirk Meyer, Robin A. Kimmel   +2 more
doaj   +1 more source

Pig islets xenotransplantation: recent progress and current perspectives

Frontiers in Surgery, 2014
Islet xenotransplantation is a prospective treatment to bridge the gap between available human cells and needs of patients with diabetes. Pig is the ideal candidate to obtain such available islet cells.
Haitao eZhu, Wanli eWang, Liang eYu, Bo eWang   +3 more
doaj   +1 more source

Islet amyloid deposition limits the viability of human islet grafts but not porcine islet grafts [PDF]

Proceedings of the National Academy of Sciences, 2010
Islet transplantation is a promising treatment for diabetes but long-term success is limited by progressive graft loss. Aggregates of the beta cell peptide islet amyloid polypeptide (IAPP) promote beta cell apoptosis and rapid amyloid formation occurs in transplanted islets.
K J, Potter, A, Abedini, P, Marek, A M, Klimek, S, Butterworth, M, Driscoll, R, Baker, M R, Nilsson, G L, Warnock, J, Oberholzer, S, Bertera, M, Trucco, G S, Korbutt, P E, Fraser, D P, Raleigh, C B, Verchere   +15 more
openaire   +2 more sources

Effect of Manufacturing Procedures on Human Islet Isolation from Donor Pancreata Standardized by the North American Islet Donor Score

Cell Transplantation, 2017
This study investigates manufacturing procedures that affect islet isolation outcomes from donor pancreata standardized by the North American Islet Donor Score (NAIDS).
Chun-Chieh Yeh, Ling-Jia Wang, James J. Mcgarrigle, Yong Wang, Chien-Chang Liao, Mustafa Omami, Arshad Khan, Mohammad Nourmohammadzadeh, Joshua Mendoza-Elias, Benjamin Mccracken, Enza Marchese, Barbara Barbaro, Jose Oberholzer   +12 more
doaj   +1 more source

Spatiotemporal Correlation Spectroscopy Reveals a Protective Effect of Peptide-Based GLP-1 Receptor Agonism against Lipotoxicity on Insulin Granule Dynamics in Primary Human β-Cells

Pharmaceutics, 2021
Glucagon-like peptide-1 receptor (GLP-1R) agonists are being used for the treatment of type 2 diabetes (T2D) and may have beneficial effects on the pancreatic β-cells.
Gianmarco Ferri, Marta Tesi, Luca Pesce, Marco Bugliani, Francesca Grano, Margherita Occhipinti, Mara Suleiman, Carmela De Luca, Lorella Marselli, Piero Marchetti, Francesco Cardarelli   +10 more
doaj   +1 more source