Results 21 to 30 of about 126,560 (317)

Machine Learning Algorithms, Applied to Intact Islets of Langerhans, Demonstrate Significantly Enhanced Insulin Staining at the Capillary Interface of Human Pancreatic β Cells

Metabolites, 2021
Pancreatic β cells secrete the hormone insulin into the bloodstream and are critical in the control of blood glucose concentrations. β cells are clustered in the micro-organs of the islets of Langerhans, which have a rich capillary network.
Louise Cottle, Ian Gilroy, Kylie Deng, Thomas Loudovaris, Helen E. Thomas, Anthony J. Gill, Jaswinder S. Samra, Melkam A. Kebede, Jinman Kim, Peter Thorn   +9 more
doaj   +1 more source

Glucolipotoxic Stress-Induced Mig6 Desensitizes EGFR Signaling and Promotes Pancreatic Beta Cell Death

Metabolites, 2023
A loss of functional beta cell mass is a final etiological event in the development of frank type 2 diabetes (T2D). To preserve or expand beta cells and therefore treat/prevent T2D, growth factors have been considered therapeutically but have largely ...
Yi-Chun Chen, Andrew J. Lutkewitte, Halesha D. Basavarajappa, Patrick T. Fueger   +3 more
doaj   +1 more source

Variation in Human Islet Viability Based on Different Membrane Integrity Stains

Cell Transplantation, 2004
Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it
M. J. Barnett, D. Mcghee-Wilson, A. M. J. Shapiro, J. R. T. Lakey Ph.D.   +3 more
doaj   +1 more source

Superiority of Small Islets in Human Islet Transplantation [PDF]

Diabetes, 2007
Many factors influence the outcome of islet transplantation. As islets in the early posttransplant setting are supplied with oxygen by diffusion only and are in a hypoxic state in the portal system, we tested whether small human islets are superior to large islets both in vitro and in vivo.
Lehmann, R, Zuellig, R A, Kugelmeier, P, Baenninger, P B, Moritz, W, Perren, A, Clavien, P A, Weber, M, Spinas, G A   +8 more
openaire   +3 more sources

A Minimally-invasive Blood-derived Biomarker of Oligodendrocyte Cell-loss in Multiple Sclerosis

EBioMedicine, 2016
Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system (CNS). Minimally invasive biomarkers of MS are required for disease diagnosis and treatment. Differentially methylated circulating-free DNA (cfDNA) is a useful biomarker
John A. Olsen, Lauren A. Kenna, Regine C. Tipon, Michael G. Spelios, Mark M. Stecker, Eitan M. Akirav   +5 more
doaj   +1 more source

Outcomes of Islet Autotransplantation in Chronic Pancreatitis Patients with Complete Acinar Atrophy

Cell Transplantation, 2020
Total pancreatectomy with islet autotransplantation (TPIAT) is a promising treatment for refractory chronic pancreatitis (CP). Pathological features of CP include progressive fibrosis in pancreas parenchyma, atrophy, and/or ductal occlusion.
Gumpei Yoshimatsu MD, PhD, Mazhar A. Kanak PhD, Srividya Vasu PhD, Kenjiro Kumano MD, PhD, Michael Lawrence PhD, Nicholas Onaca MD, Morihito Takita MD, PhD, Marlon F. Levy MD, Bashoo Naziruddin PhD   +8 more
doaj   +1 more source

Pancreatic β-Cell O-GlcNAc Transferase Overexpression Increases Susceptibility to Metabolic Stressors in Female Mice

Cells, 2021
The nutrient-sensor O-GlcNAc transferase (Ogt), the sole enzyme that adds an O-GlcNAc-modification onto proteins, plays a critical role for pancreatic β-cell survival and insulin secretion.
Ramkumar Mohan, Seokwon Jo, Elina Da Sol Chung, Eunice Oribamise, Amber Lockridge, Juan E. Abrahante-Lloréns, Hai-Bin Ruan, Xiao-Yong Yang, Emilyn U. Alejandro   +8 more
doaj   +1 more source

Novel regulators of islet function identified from genetic variation in mouse islet Ca2+ oscillations

eLife, 2023
Insufficient insulin secretion to meet metabolic demand results in diabetes. The intracellular flux of Ca2+ into β-cells triggers insulin release. Since genetics strongly influences variation in islet secretory responses, we surveyed islet Ca2+ dynamics ...
Christopher H Emfinger, Lauren E Clark, Brian Yandell, Kathryn L Schueler, Shane P Simonett, Donnie S Stapleton, Kelly A Mitok, Matthew J Merrins, Mark P Keller, Alan D Attie   +9 more
doaj   +1 more source

The regulatory effect and molecular mechanism of lncRNA Gm10451 on islet cell dysfunction in children with diabetes

Frontiers in Genetics, 2022
The dysfunction of islet β-cells is one of the causes of diabetes, and lncRNA Gm10451 is also a participant in the occurrence and the development of various diseases. This study was carried out to reveal the correlation within β-cells and Gm10451.
Jiao Wang, Jiao Wang, Li-hai Zhang, Li-hai Zhang, Yu-ming Kang, Xian-he Wang, Chun-yu Jiang   +6 more
doaj   +1 more source

Methods of Human Islet Culture for Transplantation

Cell Transplantation, 2004
The ability to maintain isolated human islet preparations in tissue culture has recently been adopted by most islet transplant centers, and improves the safety as well as the practicality of islet transplantation. Maintaining islet viability and recovery,
T. B. Murdoch, D. McGhee-Wilson, A. M. J. Shapiro, J. R. T. Lakey Ph.D.   +3 more
doaj   +1 more source