RNF13 regulates the endolysosomal pathway through interaction with the small GTPase Arl8B
The E3 ubiquitin ligase RNF13 functions as a regulatory scaffold, organizing Arl8B‐dependent complexes and tuning downstream signaling that controls endolysosomal organization and trafficking. In this model, wild‐type RNF13 associates with Arl8B on endolysosomes, whereas Arl8B (E22D/F55A) or RNF13 (L244A) variants weaken complex assembly, potentially ...
Audrey M. Sénécal +4 more
wiley +1 more source
Optimized Quantitative Bacterial Two-Hybrid (qB2H) for Protein-Protein Interaction Assessment. [PDF]
Guyot A +8 more
europepmc +1 more source
A chemo‐enzymatic colorimetric cascade for detecting ethylene glycol contamination
A chemo‐enzymatic cascade is developed for ethylene glycol (EG) detection. EG is oxidized by GoADH with C1‐mediated cofactor recycling to produce glycolaldehyde (GAL). GAL subsequently reacts with purpald to generate a detectable purple chromophore.
Tawatchai Singphongam +6 more
wiley +1 more source
Aer is a bidirectional redox sensor mediating negative chemotaxis to antibiotic-induced ROS in <i>Escherichia coli</i>. [PDF]
Bhattarai N +3 more
europepmc +1 more source
Athe_2052 is an ABC transporter substrate‐binding protein that recognizes xyloglucan heptasaccharide (XXXG), a soluble breakdown product of plant xyloglucan. The crystal structure of Athe_2052, together with binding assays and computational modeling, shows how XXXG is recognized by this sugar transporter.
Hansen Tjo +6 more
wiley +1 more source
Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by an <i>Acinetobacter baylyi</i> ADP-ISx antibiotic gene biosensor strain. [PDF]
Rhyan N +5 more
europepmc +1 more source
ABSTRACT The pejerrey (Odontesthes bonariensis) is a key species for recreational and commercial fisheries in Argentina and holds significant aquaculture potential. It has been introduced to various countries worldwide, including Japan, where intensive aquaculture has developed.
Aarón Torres‐Martínez +4 more
wiley +1 more source
Effects of aminoglycosides on macrophage viability and antimicrobial function under high-glucose exposure. [PDF]
Srinontong P +5 more
europepmc +1 more source
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang +7 more
wiley +1 more source
Design and validation of an inducible and curable EvolvR system for directed evolution in <i>Corynebacterium glutamicum</i>. [PDF]
Will PM +4 more
europepmc +1 more source

