Results 131 to 140 of about 178,982 (291)

Effect of kanamycin on expression of PA0082 (hsiA1).

, 2013
Levels of β-galactosidase activity in PAK carrying chromosomal transcriptional (A) and translational (B) fusions of the hsiA1 gene (PA0082) to lacZ [38] with increasing concentrations of kanamycin as indicated in µg/ml on the x axis.
Jack Horlick (481714), Cerith Jones (481712), Hemantha Kulasekara (481715), Alain Filloux (190708), Luke Allsopp (481713)   +4 more
core   +1 more source

Autoreactivity Versus Autoimmunity: Characteristics of Autoantigen‐Specific CD4 T Cells in Rheumatoid Arthritis and Health

Arthritis &Rheumatology, EarlyView.
Objective CD4 T cells specific for citrullinated (cit) peptides are key players in rheumatoid arthritis (RA) immunopathogenesis. Characterizing these cells and identifying features of healthy and RA‐associated autoreactivity will provide valuable insight into disease mechanisms and form the basis of immune state biomarkers to facilitate the next ...
James A. Stanway, Daniel Peters, Helen Waller, Jeremy Lakey, Henrique De Paula Lemos, Kristian Williams, Abbie Degnan, Julie Diboll, Abdul Mannan Baru, David Tough, Arthur G. Pratt, Catharien M.U. Hilkens, Amy E. Anderson, John D. Isaacs   +13 more
wiley   +1 more source

Expanding Metabolic Online Monitoring Capabilities in 96‐Deepwell Microtiter Plates by Combining Optoelectronic O2 and CO2 Sensors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT To increase the information content in early stages of bioprocess development, various technologies have been introduced for monitoring cultivations in microtiter plates (MTPs). Besides spectroscopical measurements, like in the BioLector technology, optical measurements with luminescent sensors, placed directly in the cultivation medium ...
Robert Dinger, David Pfeifer, David Flitsch, Jan P. Fischer, Clemens Lattermann, Sergey M. Borisov, Jochen Büchs   +6 more
wiley   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

Deep Learning Sensing Platform for Simultaneous Detection of Kanamycin and Kanamycin B Based on Dual‐Recognition and Catalytic Modulation Mechanism of Aptamer‐Functionalized MIL‐101 Nanozyme

Food Frontiers
Extensive antibiotic use has raised concerns over residues in food, necessitating rapid and accurate detection. This study integrates smartphone‐assisted deep learning with aptamer‐functionalized MIL‐101(Fe) nanozymes to build a dual‐recognition ...
Yadi Qin, Jie Qian, Jian Sun, Qinqin Li, Jun Yao, Minwei Zhang   +5 more
doaj   +1 more source

Combinatorial Metabolic Engineering of Alkane Biosynthesis in the Osmotolerant Yeast Debaryomyces hansenii CBS 767

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Debaryomyces hansenii is a yeast with diverse potential applications like producing oleochemicals from biomass. Yet, combinatorial metabolic engineering in D. hansenii is limited because, like many nonconventional organisms, key genomic data and genetic elements are missing. Here, we report phenotypic characterization, genomic integration loci,
Zekun Li, Nilesh Kumar Sharma, Sarah Weintraub, Eric Young   +3 more
wiley   +1 more source

Whole‐Cell Production of Resolvin E2 and 17R‐Resolvin D5 by a Double‐Oxygenating 15S‐Lipoxygenase With 5S‐Lipoxygenase Activity From Pseudobdellovibrionaceae bacterium

Biotechnology and Bioengineering, EarlyView.
A recombinant whole‐cell platform expressing a bacterial 15S‐LOX exhibiting 5S‐LOX activity enabled the stereoselective production of RvE2 and 17R‐RvD5 from 18R‐HEPE and 17R‐HDHA, achieving final concentrations of 0.57 mM and 1.48 mM from 4.0 mM substrates, respectively, under optimized conditions.
Hee‐Gyeong Lee, Kyung‐Chul Shin, Deok‐Kun Oh   +2 more
wiley   +1 more source

Characterization of Immune Responses to RBD‐SD1 Nanoparticle Vaccines Derived From ACE2‐Capable HKU5‐CoV‐2, NeoCoV, and HKU5 Merbecoviruses

Biotechnology and Bioengineering, EarlyView.
SpyCatcher‐mi3 nanoparticles presenting HKU5, NeoCoV, and HKU5‐CoV‐2 RBD‐SD1 induced strong, cross‐reactive IgG responses in immunized mice. The surrogate virus neutralization test demonstrated strong activity in sera from homologous vaccination. ABSTRACT Recent discoveries of merbecoviruses such as HKU5, NeoCoV, and HKU5‐CoV‐2 that use angiotensin ...
Peter J. Halfmann, Jeong Soo Lee, Augustine Duffy, Tong Wang, Bingcheng Huang, James Z. Kurdi, Ajay Inampudi, Natasha Chen, Elizabeth Frimpong, Carmen Rai, Yoshihiro Kawaoka, Ravi S. Kane   +11 more
wiley   +1 more source

Autonomous Control of Gene Expression Using Endogenous Transcription Signals

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Autonomous cell‐based control of heterologous gene expression can simplify batch‐culture bioprocessing by eliminating external monitoring and extrinsic control of culture conditions. Existing approaches use auto‐induction media, synthetic cell‐cell communication systems, or application‐specific biosensors.
Pakpoom Subsoontorn, Drew Endy, Olivier Borkowski   +2 more
wiley   +1 more source

Kanamycin

Tuberculosis, 2008
openaire   +2 more sources