Results 181 to 190 of about 178,982 (291)

Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis. [PDF]

Nat Commun
Vasileva DP, Chhetri HB, Hochanadel LH, Streich JC, Lagergren JH, Lane MJ, Mhatre A, Sacko O, Allemann MN, Oksuz E, Carper DL, Rubino B, Chitwood S, Schmitz ZD, Klingeman DM, Abraham PE, Giannone RJ, Jacobson DA, Michener JK.   +18 more
europepmc   +1 more source

Caffeylpyruvate hydrolase from the bioluminescent fungus Neonothopanus gardneri is the key recycling enzyme in the fungal bioluminescence pathway

The FEBS Journal, EarlyView.
Caffeic acid is a central metabolite in the fungal bioluminescence pathway. We identified and characterized caffeylpyruvate hydrolase from Neonothopanus gardneri (ngarCPH) and demonstrate its ability to hydrolyze fungal oxyluciferin into caffeic and pyruvic acids, confirming a complete and self‐sustained fungal bioluminescence cycle.
Caio K. Zamuner, Douglas M. M. Soares, Bianca B. Nóbrega, Etelvino J. H. Bechara, Zinaida M. Kaskova, Alexander S. Mishin, Karen S. Sarkisyan, Ilia V. Yampolsky, Cassius V. Stevani   +8 more
wiley   +1 more source

Precise genome modification of agrobacterium based on one-step homologous recombination via endogenous transfer DNA. [PDF]

Acta Biochim Pol
Liu Y, Sun G, Gao C, Gao R, Tang J, Wang M, Guo J, Guo R.   +7 more
europepmc   +1 more source

Unraveling in vitro phase separation and aggregation properties of the structured region of FMRP and the impact of Fragile X syndrome‐linked mutations

The FEBS Journal, EarlyView.
Fragile X messenger ribonucleoprotein 1 (FMRP) is a multidomain RNA‐binding protein associated with Fragile X Syndrome (FXS). We found that its N‐terminal structured region has an intrinsic propensity to undergo liquid–liquid phase separation and fibril formation. FXS‐associated mutations perturb protein stability and aggregation propensity, suggesting
Flavia Catalano, Alessandra Bigi, Francesca Troilo, Federica Gabriele, Gianluca Pistoia, Italia A. Asteriti, Francesco Angelucci, Giorgio Giardina, Fabrizio Chiti, Carlo Travaglini‐Allocatelli, Adele Di Matteo   +10 more
wiley   +1 more source

Association Between Antimicrobial Susceptibility Test Results and Bacteriological Cure of Mild and Moderate Bovine Clinical Mastitis. [PDF]

Vet Sci
Nery Garcia BL, Fidelis CE, Barbosa KDS, Freu G, Santos MVD.   +4 more
europepmc   +1 more source

Toward understanding the catalytic mechanism for two classes of bacterial phospholipid N‐methyltransferases

The FEBS Journal, EarlyView.
Phospholipid N‐methyltransferases (Pmts) synthesize phosphatidylcholine in many bacteria. In this study, we compared the catalytic mechanisms of two bacterial Pmt classes: the Rhodobacter (R‐) and the Sinorhizobium (S‐) type. Representative enzymes for each class were derived from Rubellimicrobium thermophilum and Agrobacterium tumefaciens ...
Irina Shevyreva, Lena Sophie Fritsch, Leander Mika Koch, Anton Effing, Meriyem Aktas, Franz Narberhaus   +5 more
wiley   +1 more source

Long-term stability of the GMP-compliant recombinant BCG vaccine against human RSV under quality control analyses. [PDF]

iScience
Troncoso-Bravo T, Rivera DB, Ramírez MA, Loaiza RA, Reyes A, Pavez V, Kourounis D, Tsatsara KP, Horefti E, Martinez-Gonzalez B, Rabias I, Peñaloza HF, González PA, Bueno SM, Kalergis AM.   +14 more
europepmc   +1 more source

Structural and thermodynamic analyses of a novel β‐1,2‐glucan binding mode in the ABC transporter solute‐binding protein Chy400_4166 from Chloroflexus aurantiacus

The FEBS Journal, EarlyView.
Cyclic β‐1,2‐glucan (CβG)‐associated solute‐binding protein of type I ABC transporter was originally identified in Chloroflexus aurantiacus, a phototrophic bacterium. Crystal structures in complex with CβGs revealed unique binding modes that are fundamentally distinct from that of the short β‐1,2‐glucooligosaccharides‐binding protein.
Kazuya Kato, Tatsuya Kaneko, Rintaro Hirayama, Nobukiyo Tanaka, Hiroyuki Nakai, Hidetaka Torigoe, Masahiro Nakajima   +6 more
wiley   +1 more source

Combinatorial action of regulatory systems generates colistin heteroresistance. [PDF]

Sci Adv
Choby JE, Crispell EK, Ma M, Vu LM, Key MK, Ernst RK, Kim M, Weiss DS.   +7 more
europepmc   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source