Results 191 to 200 of about 164,663 (253)

Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation. [PDF]

STAR Protoc
Mountourakis F, Fragkostefanakis S, Moschou PN.   +2 more
europepmc   +1 more source

Saturating mutagenesis on the Ser118 residue responsible for the spectral tuning mechanism of the orange/green‐reversible cyanobacteriochrome AM1_1499g1

Plant Biology, EarlyView.
This graphical abstract illustrates our systematic site‐saturation mutagenesis at the Ser118 position of the AM1_1499g1 GAF domain. By replacing this single residue, we generated a diverse array of variants with distinct spectral properties, demonstrating the remarkable molecular plasticity of cyanobacteriochromes and their customizability for ...
A. Yoneda, T. Suzuki, R. Narikawa
wiley   +1 more source

DNA recognition, cleavage, and toxic metal ion interaction of an artificial zinc finger protein inside E. coli cells. [PDF]

Protein Sci
Hajdu B, Tóth A, Fazekas B, Horvát M, Kato K, Kawaguchi A, Nagata K, Jancsó A, Gyurcsik B.   +8 more
europepmc   +1 more source

Genome editing and regeneration pipeline for engineering disease resistance in tomato using CRISPR/Cas9. [PDF]

Front Plant Sci
Erdoğan İ, Debbarma R, Sherry M, Mancak İ, Grant M, Tör M.   +5 more
europepmc   +1 more source

A Perspective of Soy Isoflavones on Analytical, Biological, and Chemical Attributes

Legume Science, Volume 8, Issue 3, September 2026.
ABSTRACT This review covers key aspects of soybean (Glycine max), with a particular focus on its isoflavone content and biological properties. Soybeans maintain their position as both a nutraceutical and a pharmaceutical crop, with a broad range of dietary items used worldwide, including soy milk, fermented soy products, and various dietary supplements.
Sristy Suman, Joydeep Chatterjee, Gaurav Joshi, Raj Kumar, Muhammad Wahajuddin   +4 more
wiley   +1 more source

Coexistence and Genetic Location of Aminoglycoside-Modifying Enzyme Genes in Clinical <i>Escherichia coli</i> Isolates from a Tertiary Hospital in Ouagadougou. [PDF]

Infect Drug Resist
Kouta OF, Metuor Dabire A, Bonkoungou M, Bambara LEB, Zohoncon TM.   +4 more
europepmc   +1 more source

Quaternary structures of Streptococcus pneumoniae nucleoside diphosphate kinase: From hexamers to supramolecular assemblies

Protein Science, Volume 35, Issue 9, September 2026.
Abstract The nucleoside diphosphate kinase (NDK) is a key enzyme that controls the balance of nucleotide pools in all living organisms. Beyond this fundamental role, NDKs exert pleiotropic effects in many cellular processes, including cell development, signal transduction, differentiation, tumor metastasis, and gene expression. The quaternary structure
Julie Kerboeuf, Paul Nouri, Frédéric Galisson, Laetitia Daury, Marie‐France Giraud, Olivier Lambert, Cédric Orelle, Lionel Ballut, Elise Kaplan, Jean‐Michel Jault, Cécile Gonzalez   +10 more
wiley   +1 more source

Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system. [PDF]

Nat Microbiol
Ong S, Ghode P, Narenderan A, Lao S, Willenborg F, Eden TV, Marsh CO, Yew WS, Fredens J.   +8 more
europepmc   +1 more source

Facile, high‐throughput protein purification enabled by high‐efficiency protease elution

Protein Science, Volume 35, Issue 9, September 2026.
Abstract While advances in artificial intelligence have made protein design widely accessible, protein production and characterization remain a bottleneck. Protease‐cleavable affinity tags are commonly used to improve yield and purity of recombinant proteins, but tag removal adds labor and complexity.
Edson N. Cárcamo Noriega, Ian S. Truebridge, Frank D. Teets III, James W. Bowman III, Nathalia Rodriguez, Tessa A. Howard, Christopher D. Bahl   +6 more
wiley   +1 more source

Evolutionary insights into bilin biosynthesis: Functional characterization of pre‐PcyA enzymes

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Biosynthesis of the linear tetrapyrrole phycocyanobilin (PCB) by the ferredoxin‐dependent bilin reductase (FDBR) PcyA is essential for light‐harvesting and regulatory processes in diverse photosynthetic organisms, yet its evolutionary origins are not fully understood. PcyA evolved from pre‐PcyA proteins found in diverse bacteria.
Federica Frascogna, Nathan C. Rockwell, Gunhild Layer, Nicole Frankenberg‐Dinkel   +3 more
wiley   +1 more source