Results 41 to 50 of about 164,663 (253)
Advanced Science, EarlyView.This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu +10 more
wiley +1 more source
Kanamycin and G-Quadruplexes: An Exploration of Binding Interactions
MoleculesG-quadruplexes (G4s) are distinctive four-stranded nucleic acid structures formed by guanine-rich sequences, making them attractive targets for drug repurposing efforts.
Gianmarco Gualtieri +6 more
doaj +1 more source
Modified Aminoglycosides Bind Nucleic Acids in High-Molecular-Weight Complexes
Antibiotics, 2020 Aminoglycosides represent a large group of antibiotics well known for their ability to target the bacterial ribosome. In studying 6”-substituted variants of the aminoglycoside tobramycin, we serendipitously found that compounds with C12 or C14 ...
Lanqing Ying +4 more
doaj +1 more source
Advanced Science, EarlyView.Ubiquilin (UBQLN), like many other human proteins, contains both well‐folded and disordered regions. Here, we show that intramolecular interactions between disordered regions and folded domains modulate between open and closed topologies of UBQLN proteins, altering their structure and function.
Jessica K. Niblo +4 more
wiley +1 more source
Factors affecting gene transformation in mangosteen [PDF]
Songklanakarin Journal of Science and Technology (SJST), 2003 Factors affecting gene transformation in mangosteen (Garcinia mangostana L.) were investigated. Types of explants, strains and densities of Agrobacterium tumefaciens, and co-culture methods were examined to optimize gene transformation.
Sompong Te-chato, Vitoon Chaipakdee
doaj
Resistant Peanut Genotype Reprograms Rhizosphere Metabolism to Enhance Bacterial Wilt Suppression
Advanced Science, EarlyView.The resistant peanut genotype selectively recruits beneficial bacteria, which coincides with the activation of salicylic acid (SA)‐dependent systemic acquired resistance (SAR) against Ralstonia solanacearum. Keystone rhizosphere metabolites are positively correlated with both beneficial microbiome assembly and SAR gene expression.
Rui Ren +20 more
wiley +1 more source
Advanced Science, EarlyView.Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan +7 more
wiley +1 more source
Theoretical Studies on Mechanism of Inactivation of Kanamycin A by 4′-O-Nucleotidyltransferase
Frontiers in Chemistry, 2019 This work is focused on mechanistic studies of the transfer of an adenylyl group (Adenoside-5′-monophosfate) from adenosine 5′-triphosphate (ATP) to a OH-4′ hydroxyl group of an antibiotic.
Sergio Martí +2 more
doaj +1 more source
Advanced Science, EarlyView.Phylogenetic and biochemical analyses of the heme transporter CydDC reveal its functional conservation throughout bacterial evolution and demonstrate its unique asymmetric allosteric mechanism. Furthermore, impairment of CydDC function directly affects bacterial antibiotic resistance and likely compromises antibiotic efficacy through drug efflux.
Lili Yang +19 more
wiley +1 more source
Frontiers in PharmacologyBackgroundMethyltransferase-like 3 (METTL3), a component of the N6-methyladenosine (m6A) methyltransferase family, exhibits significant expression in HEI-OC1 cells and cochlear explants.
Yan Wu +13 more
doaj +1 more source