Results 71 to 80 of about 10,759 (233)

Dimethylmethylene Blue Assay (DMMB)

Bio-Protocol, 2014
Glycosaminoglycans (GAGs) are long unbranched polysaccharides consisting of repeating disaccharide units composed of a hexosamine (glucosamine or galactosamine) and a hexuronic acid (glucuronic or iduronic acid).
Vivien Coulson-Thomas, Tarsis Ferreira
doaj   +1 more source

Single‐cell spatial transcriptomic sequencing and machine learning models uncover molecular mechanisms and diagnostic biomarkers of Peyronie's disease formation in rats

VIEW, EarlyView.
Peyronie's disease (PD) is a common male reproductive system disorder. The exact etiology of PD remains unclear. This study aimed to investigate molecular and cellular changes in PD using single‐cell spatial transcriptomics. Abstract Peyronie's disease (PD), commonly known as penile induration, is a prevalent male reproductive system disorder with ...
Keyuan Lou, Qiancheng Mao, Junpeng Chi, Jiankun Zhang, Hao Guo, Jitao Wu, Yuanshan Cui   +6 more
wiley   +1 more source

Enzymatic sulfation of galactose residue of keratan sulfate by chondroitin 6-sulfotransferase [PDF]

Glycobiology, 1996
We have previously found that the purified chondroitin 6-sulfotransferase (C6ST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of N-acetylgalactosamine in chondroitin, catalyzed the sulfation of keratan sulfate, and that both the C6ST activity and the keratan sulfate sulfotransferase (KSST) activity were ...
Kenji Uchimura, Yukie Hirahara, Masakazu Fukuta, Osami Habuchi   +3 more
openaire   +3 more sources

N-Acetylglucosamine 6-O-Sulfotransferase-1-Deficient Mice Show Better Functional Recovery after Spinal Cord Injury [PDF]

, 2010
名古屋大学NAGOYA University博士(医学)Neurons in the adult CNS do not spontaneously regenerate after injuries. The glycosaminoglycan keratan sulfate is induced after spinal cord injury, but its biological significance is not well understood.
41606, 41607, 41608, 41609, 41610, 41611, 41612, 41613, 41614, 41615, Ando, Kei, Hirano, Kenichi, Imagama, Shiro, Ishiguro, Naoki, ITO, Zenya, Kadomatsu, Kenji, Matsuyama, Yukihiro, Sakamoto, Kazuma, Yamashita, Toshihide, Zhang, Haoqian   +19 more
core   +2 more sources

Contaminants in Commercial Preparations of ‘Purified’ Small Leucine-Rich Proteoglycans May Distort Mechanistic Studies [PDF]

, 2016
This paper reports the perplexing results that came about because of seriously impure commercially available reagents. Commercial reagents and chemicals are routinely ordered by scientists and are expected to have been rigorously assessed for their ...
Botting, CH, Caterson, B, Fuller, HR, Jones, PW, Owen, SJ, Roberts, S, Shirran, SL   +6 more
core   +6 more sources

Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production

BMC Biotechnology, 2008
Background The meniscus is a complex tissue whose cell biology has only recently begun to be explored. Published models rely upon initial culture in the presence of added growth factors. The aim of this study was to test a three-dimensional (3D) collagen
Norton H James, Ingram Jane A, Chow Yin, Mauerhan David, Gruber Helen E, Hanley Edward N, Sun Yubo   +6 more
doaj   +1 more source

Distribution and Function of Glycosaminoglycans and Proteoglycans in the Development, Homeostasis and Pathology of the Ocular Surface

Frontiers in Cell and Developmental Biology, 2020
The ocular surface, which forms the interface between the eye and the external environment, includes the cornea, corneoscleral limbus, the conjunctiva and the accessory glands that produce the tear film.
Sudan Puri, Yvette M. Coulson-Thomas, Tarsis F. Gesteira, Tarsis F. Gesteira, Vivien J. Coulson-Thomas   +4 more
doaj   +1 more source

Malformations of cortical development: Embryology and epilepsy

Epilepsia, EarlyView.
Abstract One in seven patients with focal epilepsy has a malformation of cortical development (MCD) as underlying cause. Understanding normal cortical development combined with knowledge of where, when, and what goes wrong in different types of MCD provides insight into the mechanisms of epileptogenesis.
M. Christianne Hoeberigs, Simon Tousseyn, on behalf of the ACE Study Group, Gwendolyn de Bruyn, Albert Colon, Jim Dings, Marc Hendriks, Danny Hilkman, Christianne Hoeberigs, Jochem van der Pol, Lotte de Jong, Kim Rijkers, Sylvia Klinkenberg, Vivianne van Kranen Mastenbroek, Jeske Nelissen, Pieter Kubben, Walter M. Palm, Rob P. W. Rouhl, Remco Santegoeds, Olaf Schijns, Ilse van Straaten, Marielle Vlooswijk, Louis Wagner, Dorien Weckhuysen   +23 more
wiley   +1 more source

Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan [PDF]

, 1987
Three antibodies reacting with corneal keratan sulfate proteoglycan were used to detect antigenically related molecules in 11 bovine and 13 embryonic chick tissues. Two monoclonal antibodies recognized sulfated epitopes on the keratan sulfate chain and a
Caterson, B, Conrad, G W, Funderburgh, J L   +2 more
core   +1 more source

Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases [PDF]

, 2013
Purpose. Extracellular matrix metalloproteinases (MMPs) are thought to play a crucial role in corneal degradation associated with the pathological progression of keratoconus.
Conrad, Gary W., Littlechild, Stacy Lee, Mao, Xiuli, Schwend, Tyler, Zhang, Yuntao   +4 more
core   +1 more source