Results 101 to 110 of about 94,650 (353)
Development, 2019 Drosophila Ensconsin (also known as MAP7) controls spindle length, centrosome separation in brain neuroblasts (NBs) and asymmetric transport in oocytes.
Mathieu Métivier +8 more
semanticscholar +1 more source
Dissection of Kinesin's Processivity
PLoS ONE, 2009 The protein family of kinesins contains processive motor proteins that move stepwise along microtubules. This mechanism requires the precise coupling of the catalytic steps in the two heads, and their precise mechanical coordination. Here we show that these functionalities can be uncoupled in chimera of processive and non-processive kinesins. A chimera
Günther Woehlke +5 more
openaire +5 more sources
ATP Hydrolysis by α‐Synuclein Amyloids is Mediated by Enclosing β‐Strand
Advanced Science, EarlyView.Pathological amyloids have been considered chemically inert until recently. Here it is shown that α‐synuclein amyloids catalyze the hydrolysis of the universal energy molecule, ATP. The cryo‐EM structure of the complex reveals an additional β‐strand (purple) that encloses the ATP‐binding site. Within the cavity, several lysine residues are required for
Lukas Frey +6 more
wiley +1 more source
Effect of 2-H and 18-O water isotopes in kinesin-1 gliding assay [PDF]
, 2012 We show here the effects of heavy-hydrogen water (^2^H~2~O) and heavy-oxygen water (H~2~^18^O) on the gliding speed of microtubules on kinesin-1 coated surfaces.
Andy Maloney +2 more
core +1 more source
On the motion of kinesin in a viscoelastic medium
, 2017 Kinesin is a molecular motor that transports cargo along microtubules. The results of many {\it in vitro} experiments on kinesin-1 are described by kinetic models \cite{Clancy11} in which one transition corresponds to the forward motion and subsequent ...
Knoops, Gert, Vanderzande, Carlo
core +1 more source
Interaction of heterotrimeric kinesin-II with IFT-B–connecting tetramer is crucial for ciliogenesis
Journal of Cell Biology, 2018 Intraflagellar transport (IFT) is crucial for the assembly and maintenance of cilia and is mediated by IFT particles containing IFT-A and IFT-B complexes.
Teruki Funabashi +4 more
semanticscholar +1 more source
Advanced Science, EarlyView.The quantitative proteomic and phosphoproteomic profiling reveals dynamic phosphorylation regulation of sperm morphogenesis. Kinase‐substrate phosphorylation network and phosphorylation module analysis, followed by in vivo knockdown and knockout analysis, identify TTBK2 and CSNK1G1 as key regulators of morphogenesis, including head, flagellar, and ...
Tianyu Zhu +25 more
wiley +1 more source
Cell ReportsSummary: Each cargo in a cell employs a unique set of motor proteins for its transport. To dissect the roles of each type of motor, we developed optogenetic inhibitors of endogenous kinesin-1, -2, -3 and dynein motors and examined their effect on the ...
Sahil Nagpal +7 more
doaj +1 more source
The mammalian dynein/dynactin complex is a strong opponent to kinesin in a tug-of-war competition
Nature Cell Biology, 2016 Kinesin and dynein motors transport intracellular cargos bidirectionally by pulling them in opposite directions along microtubules, through a process frequently described as a ‘tug of war’. While kinesin produces 6 pN of force, mammalian dynein was found
V. Belyy +5 more
semanticscholar +1 more source
Enhanced Single‐Particle Upconversion Imaging via Energy Migration Boosting
Advanced Science, EarlyView.A core‐shell‐shell Yb3⁺/Er3⁺ UCNP architecture is engineered to balance energy migration and back energy transfer, yielding more than a tenfold enhancement in single‐particle brightness. Leveraging these optimized probes, it achieves long‐term, high‐resolution single‐particle tracking in live neurons, uncovering coordinated transport mechanisms of ...
Yanxin Zhang +7 more
wiley +1 more source