Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]
Open Biology, 2016 Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
doaj +2 more sources
Molecular basis of outer kinetochore assembly on CENP-T
eLife, 2016 Stable kinetochore-microtubule attachment is essential for cell division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins C and T (CENP-C and CENP-T).
Pim J Huis in 't Veld +8 more
doaj +2 more sources
Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore
Nature Communications, 2016 Chromosomes bind microtubules (MT) from opposite spindle poles and the generated tension stabilizes kinetochore-MT attachments. Here the authors measure kinetochore forces by engineering two force sensors and propose that kinetochore fibers exert ...
Anna A. Ye +2 more
doaj +2 more sources
Recent advances in plant kinetochore research [PDF]
Frontiers in Cell and Developmental BiologyFaithful chromosome segregation is crucial for cell division in eukaryotes, facilitated by the kinetochore, a multi-subunit protein complex that connects chromosomes to the spindle microtubules.
Elena Kozgunova, Elena Kozgunova
doaj +2 more sources
Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation. [PDF]
PLoS GeneticsThe kinetochore, a megadalton structure composed of centromeric (CEN) DNA and protein complexes, is required for faithful chromosome segregation in eukaryotes.
Prashant K Mishra +11 more
doaj +2 more sources
Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80
eLife, 2017 Chromosome segregation requires robust interactions between the macromolecular kinetochore structure and dynamic microtubule polymers. A key outstanding question is how kinetochore-microtubule attachments are modulated to ensure that bi-oriented ...
David M Kern +4 more
doaj +2 more sources
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome
bioRxiv, 2022 Accurate chromosome segregation, controlled by kinetochore-mediated chromatid attachments to the mitotic spindle, ensures the faithful inheritance of genetic information.
S. Yatskevich +9 more
semanticscholar +1 more source
Cell cycle control of kinetochore assembly
Nucleus, 2022 The kinetochore is a large proteinaceous structure assembled on the centromeres of chromosomes. The complex machinery links chromosomes to the mitotic spindle and is essential for accurate chromosome segregation during cell division.
Qianhua Dong, Fei Li
doaj +1 more source
Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution
Frontiers in Cell and Developmental Biology, 2022 The assembly of a functional kinetochore on centromeric chromatin is necessary to connect chromosomes to the mitotic spindle, ensuring accurate chromosome segregation.
Shreyas Sridhar, Tatsuo Fukagawa
doaj +1 more source
A coordinated interdependent protein circuitry stabilizes the kinetochore ensemble to protect CENP-A in the human pathogenic yeast Candida albicans. [PDF]
PLoS Genetics, 2012 Unlike most eukaryotes, a kinetochore is fully assembled early in the cell cycle in budding yeasts Saccharomyces cerevisiae and Candida albicans. These kinetochores are clustered together throughout the cell cycle.
Jitendra Thakur, Kaustuv Sanyal
doaj +1 more source