Sad1 Spatiotemporally Regulates Kinetochore Clustering To Ensure High-Fidelity Chromosome Segregation in the Human Fungal Pathogen
mSphere, 2018 Kinetochore clustering, frequently observed in yeasts, plays a key role in genome organization and chromosome segregation. In the absence of the metaphase plate arrangement, kinetochore clustering in yeast species is believed to facilitate timely ...
Vikas Yadav, Kaustuv Sanyal
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Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]
Open Biology, 2016 Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
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Molecular basis of outer kinetochore assembly on CENP-T
eLife, 2016 Stable kinetochore-microtubule attachment is essential for cell division. It requires recruitment of outer kinetochore microtubule binders by centromere proteins C and T (CENP-C and CENP-T).
Pim J Huis in 't Veld +8 more
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Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore
Nature Communications, 2016 Chromosomes bind microtubules (MT) from opposite spindle poles and the generated tension stabilizes kinetochore-MT attachments. Here the authors measure kinetochore forces by engineering two force sensors and propose that kinetochore fibers exert ...
Anna A. Ye +2 more
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Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80
eLife, 2017 Chromosome segregation requires robust interactions between the macromolecular kinetochore structure and dynamic microtubule polymers. A key outstanding question is how kinetochore-microtubule attachments are modulated to ensure that bi-oriented ...
David M Kern +4 more
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Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation. [PDF]
PLoS GeneticsThe kinetochore, a megadalton structure composed of centromeric (CEN) DNA and protein complexes, is required for faithful chromosome segregation in eukaryotes.
Prashant K Mishra +11 more
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Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome
bioRxiv, 2022 Accurate chromosome segregation, controlled by kinetochore-mediated chromatid attachments to the mitotic spindle, ensures the faithful inheritance of genetic information.
S. Yatskevich +9 more
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Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution
Frontiers in Cell and Developmental Biology, 2022 The assembly of a functional kinetochore on centromeric chromatin is necessary to connect chromosomes to the mitotic spindle, ensuring accurate chromosome segregation.
Shreyas Sridhar, Tatsuo Fukagawa
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Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
bioRxiv, 2022 Summary The point centromere of budding yeast specifies assembly of the large multi-subunit kinetochore complex. By direct attachment to the mitotic spindle, kinetochores couple the forces of microtubule dynamics to power chromatid segregation at mitosis.
Tom Dendooven +7 more
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Cell cycle control of kinetochore assembly
Nucleus, 2022 The kinetochore is a large proteinaceous structure assembled on the centromeres of chromosomes. The complex machinery links chromosomes to the mitotic spindle and is essential for accurate chromosome segregation during cell division.
Qianhua Dong, Fei Li
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