Trypanosome KKIP1 Dynamically Links the Inner Kinetochore to a Kinetoplastid Outer Kinetochore Complex [PDF]
Frontiers in Cellular and Infection Microbiology, 2021 Kinetochores perform an essential role in eukaryotes, coupling chromosomes to the mitotic spindle. In model organisms they are composed of a centromere-proximal inner kinetochore and an outer kinetochore network that binds to microtubules.
Lorenzo Brusini +6 more
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Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore [PDF]
Scientific Reports, 2017 The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat +3 more
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Native yeast kinetochore structures identify an essential inner kinetochore interaction [PDF]
Nature CommunicationsKinetochores must accurately assemble on centromeres for faithful chromosome segregation. Although a conserved centromeric nucleosome is essential for kinetochore assembly, budding yeast centromeric DNA is a poor template for nucleosome formation in ...
Mengqiu Jiang +6 more
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Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore
Nature Communications, 2016 Chromosomes bind microtubules (MT) from opposite spindle poles and the generated tension stabilizes kinetochore-MT attachments. Here the authors measure kinetochore forces by engineering two force sensors and propose that kinetochore fibers exert ...
Anna A Ye, Thomas J Maresca
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Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore [PDF]
Nature CommunicationsMultiple microtubule-directed activities concentrate on mitotic chromosomes to ensure their faithful segregation. These include couplers and dynamics regulators localized at the kinetochore, the microtubule interface built on centromeric chromatin, as ...
Bram Prevo +3 more
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The path of DNA in the kinetochore [PDF]
Current Biology, 2006 The kinetochore is the protein-DNA complex at eukaryotic centromeres that functions as the attachment site for spindle microtubules. In budding yeast, the centromere spans 120 bp, there is a single microtubule per kinetochore, and the entire spindle is composed of 16 kinetochore microtubules plus four interpolar microtubules from each pole.
Bloom, Kerry +2 more
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Poleward force at the kinetochore in metaphase depends on the number of kinetochore microtubules. [PDF]
The Journal of cell biology, 1990 To examine the dependence of poleward force at a kinetochore on the number of kinetochore microtubules (kMTs), we altered the normal balance in the number of microtubules at opposing homologous kinetochores in meiosis I grasshopper spermatocytes at metaphase with a focused laser microbeam.
T S, Hays, E D, Salmon
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Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution
Frontiers in Cell and Developmental Biology, 2022 The assembly of a functional kinetochore on centromeric chromatin is necessary to connect chromosomes to the mitotic spindle, ensuring accurate chromosome segregation.
Shreyas Sridhar, Tatsuo Fukagawa
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Cell cycle control of kinetochore assembly
Nucleus, 2022 The kinetochore is a large proteinaceous structure assembled on the centromeres of chromosomes. The complex machinery links chromosomes to the mitotic spindle and is essential for accurate chromosome segregation during cell division.
Qianhua Dong, Fei Li
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A coordinated interdependent protein circuitry stabilizes the kinetochore ensemble to protect CENP-A in the human pathogenic yeast Candida albicans. [PDF]
PLoS Genetics, 2012 Unlike most eukaryotes, a kinetochore is fully assembled early in the cell cycle in budding yeasts Saccharomyces cerevisiae and Candida albicans. These kinetochores are clustered together throughout the cell cycle.
Jitendra Thakur, Kaustuv Sanyal
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