Results 11 to 20 of about 105,994 (204)

Visualizing kinetochore architecture [PDF]

Current Opinion in Structural Biology, 2011
Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo.
Gregory, Alushin, Eva, Nogales
openaire   +2 more sources

DNA content of a functioning chicken kinetochore [PDF]

, 2014
© The Author(s) 2014. In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and ...
A Alonso, AE Barry, AF Pluta, AP Joglekar, BA Sullivan, BR Brinkley, CM Wade, D Gerlich, D Sart du, D Varma, DR Foltz, FG Westhorpe, H Maiato, H Saitoh, IM Cheeseman, J Dekker, JH Hegemann, K Jaqaman, LL Sullivan, LS Burrack, MD Blower, N Naumova, O Capozzi, OJ Marshall, P Luykx, P Vagnarelli, Paola Vagnarelli, PT Jokelainen, R Saffery, SA Ribeiro, SA Ribeiro, Susana Abreu Ribeiro, T Fukagawa, T Hori, WC Earnshaw, WC Earnshaw, WH Shang, WH Shang, William C. Earnshaw, X Wan   +39 more
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Chromosome Segregation Is Biased by Kinetochore Size [PDF]

, 2018
Chromosome missegregation during mitosis or meiosis is a hallmark of cancer and the main cause of prenatal death in humans. The gain or loss of specific chromosomes is thought to be random, with cell viability being essentially determined by selection ...
Aguiar, P, Almeida, A C, Damas, J, Drpic, D, Khodjakov, A, Larkin, D M, Lewin, H A, Maiato, H, Renda, F   +8 more
core   +2 more sources

The Kinetochore [PDF]

Cold Spring Harbor Perspectives in Biology, 2014
A critical requirement for mitosis is the distribution of genetic material to the two daughter cells. The central player in this process is the macromolecular kinetochore structure, which binds to both chromosomal DNA and spindle microtubule polymers to direct chromosome alignment and segregation. This review will discuss the key kinetochore activities
openaire   +2 more sources

Kinetochore inactivation by expression of a repressive mRNA. [PDF]

, 2017
Differentiation programs such as meiosis depend on extensive gene regulation to mediate cellular morphogenesis. Meiosis requires transient removal of the outer kinetochore, the complex that connects microtubules to chromosomes.
Chen, Jingxun, Chia, Minghao, Jorgensen, Victoria, Liao, Hanna, McSwiggen, David T, Spedale, Gianpiero, Tresenrider, Amy, van Werven, Folkert Jacobus, Ünal, Elçin   +8 more
core   +2 more sources

Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner. [PDF]

, 2019
The spindle assembly checkpoint (SAC) prevents anaphase until all kinetochores attach to the spindle. Each mammalian kinetochore binds many microtubules, but how many attached microtubules are required to turn off the checkpoint, and how the kinetochore ...
Akiyoshi, Alushin, Aravamudhan, Ballister, Barisic, Cane, Cheeseman, Chen, Ciferri, Clute, David, De Antoni, DeLuca, DeLuca, DeLuca, Dick, Dudka, Elting, Elting, Etemad, Etemad, Funabiki, Gassmann, Guimaraes, Hengeveld, Hewitt, Hiruma, Howell, Howell, Hueschen, Janssen, Jelluma, Ji, Johnston, Jonathan Kuhn, Kajtez, Khodjakov, Kuhn, London, Long, Lukinavičius, Maciejowski, Magidson, Maldonado, Maresca, Martin-Lluesma, Matson, McEwen, McIntosh, McKinley, Nijenhuis, Orth, O’Connell, Peterson, Pinsky, Rieder, Rieder, Santaguida, Sarangapani, Saurin, Shah, Sikirzhytski, Sikirzhytski, Sivakumar, Sophie Dumont, Sundin, Suzuki, Suzuki, Tanaka, Tauchman, Uchida, Uetake, Vanoosthuyse, Volkov, Wan, Waters, Wendell, Wojcik, Yoo, Zaytsev, Zaytsev   +80 more
core   +1 more source

Kinetochore-bound Mps1 regulates kinetochore–microtubule attachments via Ndc80 phosphorylation [PDF]

Journal of Cell Biology, 2021
Dividing cells detect and correct erroneous kinetochore–microtubule attachments during mitosis, thereby avoiding chromosome missegregation. The Aurora B kinase phosphorylates microtubule-binding elements specifically at incorrectly attached kinetochores, promoting their release and providing another chance for proper attachments to form.
Krishna K. Sarangapani, Lori B. Koch, Christian R. Nelson, Charles L. Asbury, Sue Biggins   +4 more
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Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]

Open Biology, 2016
Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
doaj   +1 more source

The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]

Open Biology, 2015
The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas, Tina R. Lynch, Ryan Anderson, Sana Afreen, Dileep Varma, Jennifer G. DeLuca   +5 more
doaj   +1 more source

Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments [PDF]

, 2015
We previously reported that the spindle assembly checkpoint protein Bub3 is involved in regulating kinetochore-microtubule (KT-MT) attachments. Also, Bub3 was reported to interact with the microtubule motor protein dynein.
Barisic, Efimov, Gassmann, Gassmann, Gassmann, Hassan Bousbaa, Kops, Lo, Logarinho, Logarinho, Maia, Maresca, Musacchio, Patrícia M.A. Silva, Pfarr, Reis, Rieder, Sharp, Silva, Silva, Stehman, Steuer, Uchida, Vaisberg, Varma, Waters, Yang, Álvaro A. Tavares   +27 more
core   +1 more source