Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore [PDF]
Scientific Reports, 2017 The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat +3 more
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Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis [PDF]
, 2010 We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions.
DeLuca, Jennifer G. +11 more
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Cold Spring Harbor Perspectives in Biology, 2014 A critical requirement for mitosis is the distribution of genetic material to the two daughter cells. The central player in this process is the macromolecular kinetochore structure, which binds to both chromosomal DNA and spindle microtubule polymers to direct chromosome alignment and segregation. This review will discuss the key kinetochore activities
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Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation [PDF]
, 2015 During mitosis, Bub1 kinase phosphorylates histone H2A-T120 to promote centromere sister chromatid cohesion through recruitment of shugoshin (Sgo) proteins. The regulation and dynamics of H2A-T120 phosphorylation are poorly understood. Using quantitative
Lajeunesse, Audrey +6 more
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Bod1, a novel kinetochore protein required for chromosome biorientation [PDF]
, 2007 We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein
McClelland, Sarah E. +25 more
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Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]
, 2012 Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Tobias Ulbricht +47 more
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Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly [PDF]
, 2009 Mitotic spindle formation and chromosome segregation depend critically on kinetochore-microtubule (KT-MT) interactions. A new protein, termed Spindly in Drosophila and SPDL-1 in C.
Erich A. Nigg +15 more
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Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly [PDF]
, 2009 In animal somatic cells, bipolar spindle formation requires separation of the centrosome-based spindle poles. Centrosome separation relies on multiple pathways, including cortical forces and antiparallel microtubule (MT) sliding, which are two activities
Winter, Jennifer R. +11 more
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The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells [PDF]
Chromosoma, 2006 Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells.
Maiato, H +6 more
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The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]
Open Biology, 2015 The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas +5 more
doaj +1 more source