Results 11 to 20 of about 89,793 (256)
The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]
Open Biology, 2015 The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas +5 more
doaj +1 more source
Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
Scientific Reports, 2017 The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat +3 more
doaj +1 more source
Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase. [PDF]
PLoS ONE, 2014 In the budding yeast, centromeres stay clustered near the spindle pole bodies (SPBs) through most of the cell cycle. This SPB-centromere proximity requires microtubules and functional kinetochores, which are protein complexes formed on the centromeres ...
Soumitra Sau +3 more
doaj +1 more source
Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.
PLoS Genetics, 2016 The accurate segregation of chromosomes during cell division is achieved by attachment of chromosomes to the mitotic spindle via the kinetochore, a large multi-protein complex that assembles on centromeres.
Eva Herrero, Peter H Thorpe
doaj +1 more source
PLoS Genetics, 2020 The kinetochore, a multi-protein complex assembled on centromeres, is essential to segregate chromosomes during cell division. Deficiencies in kinetochore function can lead to chromosomal instability and aneuploidy-a hallmark of cancer cells. Kinetochore
Guðjón Ólafsson, Peter H Thorpe
doaj +1 more source
The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells [PDF]
Chromosoma, 2006 Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells.
Maiato, H +6 more
openaire +3 more sources
Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore [PDF]
Open Biology, 2012 The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and
Zsolt Venkei +7 more
doaj +1 more source
STAR Protocols, 2021 Summary: This protocol measures the 3D Euclidean distance (Δ3D) between two/three fluorescently labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.0.1, MATLAB based).
Tsvetelina E. Germanova +4 more
doaj +1 more source
Visualizing kinetochore architecture [PDF]
Current Opinion in Structural Biology, 2011 Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo.
Gregory, Alushin, Eva, Nogales
openaire +2 more sources
Tumor Biology, 2017 Spindle and kinetochore–associated protein 1 is a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation.
Kai Wang +5 more
doaj +1 more source