Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase. [PDF]
PLoS ONE, 2014 In the budding yeast, centromeres stay clustered near the spindle pole bodies (SPBs) through most of the cell cycle. This SPB-centromere proximity requires microtubules and functional kinetochores, which are protein complexes formed on the centromeres ...
Soumitra Sau +3 more
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Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes [PDF]
, 2007 The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and generate diffusible anaphase inhibitors.
Rischitor, Patricia E +8 more
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Visualizing kinetochore architecture [PDF]
Current Opinion in Structural Biology, 2011 Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo.
Gregory, Alushin, Eva, Nogales
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The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]
, 2005 During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V. +6 more
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Tumor Biology, 2017 Spindle and kinetochore–associated protein 1 is a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation.
Kai Wang +5 more
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Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore [PDF]
Open Biology, 2012 The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and
Zsolt Venkei +7 more
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STAR Protocols, 2021 Summary: This protocol measures the 3D Euclidean distance (Δ3D) between two/three fluorescently labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.0.1, MATLAB based).
Tsvetelina E. Germanova +4 more
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Integrating Functions at the Kinetochore [PDF]
Cell, 2002 Kinetochore components have catalytic as well as structural activities. New evidence illustrates how these proteins integrate spindle morphogenesis with regulation of the timing and accuracy of chromosome segregation.
Shimoda, Soni L., Solomon, Frank
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Kinetochore-localized PP1 prematurely silences the spindle checkpoint in meiosis.
, 2023 A) Representative time-lapse images of a SPC105WT cell completing both meiotic divisions. Time 0 indicates SPB separation. Scale bar = 5 μm. B) Graph showing the duration of metaphase I and metaphase II in SPC105WT cells with and without the anchor away ...
Soni Lacefield (573500) +2 more
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Tension Management in the Kinetochore [PDF]
Current Biology, 2010 The kinetochore is the protein machine built at the centromere that integrates mechanical force and chemical energy from dynamic microtubules into directed chromosome motion. The kinetochore also provides a powerful signaling function that is able to alter the properties of the spindle checkpoint and initiate a signal transduction cascade that leads to
Bloom, Kerry, Yeh, Elaine
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