Results 21 to 30 of about 98,344 (203)
Biochemical evidence for diverse strategies in the inner kinetochore [PDF]
Open Biology, 2020 The kinetochore is a complex structure whose function is absolutely essential. Unlike the centromere, the kinetochore at first appeared remarkably well conserved from yeast to humans, especially the microtubule-binding outer kinetochore.
G. E. Hamilton, T. N. Davis
doaj +1 more source
The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein. [PDF]
PLoS Genetics, 2013 The kinetochore is the macromolecular complex that assembles onto centromeric DNA and orchestrates the segregation of duplicated chromosomes. More than 60 components make up the budding yeast kinetochore, including inner kinetochore proteins that bind to
Bungo Akiyoshi +5 more
doaj +1 more source
PLoS Genetics, 2021 The spindle assembly checkpoint (SAC) prevents anaphase onset in response to chromosome attachment defects, and SAC silencing is essential for anaphase onset. Following anaphase onset, activated Cdc14 phosphatase dephosphorylates the substrates of cyclin-
Michael Bokros +3 more
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Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis [PDF]
, 2010 We have studied Sds22, a conserved regulator of protein phosphatase 1 (PP1) activity, and determined its role in modulating the activity of aurora B kinase and kinetochore-microtubule inter-actions.
DeLuca, Jennifer G. +11 more
core +1 more source
Cold Spring Harbor Perspectives in Biology, 2014 A critical requirement for mitosis is the distribution of genetic material to the two daughter cells. The central player in this process is the macromolecular kinetochore structure, which binds to both chromosomal DNA and spindle microtubule polymers to direct chromosome alignment and segregation. This review will discuss the key kinetochore activities
openaire +2 more sources
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation [PDF]
, 2015 During mitosis, Bub1 kinase phosphorylates histone H2A-T120 to promote centromere sister chromatid cohesion through recruitment of shugoshin (Sgo) proteins. The regulation and dynamics of H2A-T120 phosphorylation are poorly understood. Using quantitative
Lajeunesse, Audrey +13 more
core +1 more source
Bod1, a novel kinetochore protein required for chromosome biorientation [PDF]
, 2007 We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein
McClelland, Sarah E. +25 more
core +1 more source
DNA content of a functioning chicken kinetochore [PDF]
, 2014 In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments ...
Vagnarelli, Paola +8 more
core +1 more source
Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]
Open Biology, 2016 Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
doaj +1 more source
Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]
, 2012 Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Tobias Ulbricht +47 more
core +1 more source