Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly [PDF]
, 2009 Mitotic spindle formation and chromosome segregation depend critically on kinetochore-microtubule (KT-MT) interactions. A new protein, termed Spindly in Drosophila and SPDL-1 in C.
Erich A. Nigg +15 more
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Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly [PDF]
, 2009 In animal somatic cells, bipolar spindle formation requires separation of the centrosome-based spindle poles. Centrosome separation relies on multiple pathways, including cortical forces and antiparallel microtubule (MT) sliding, which are two activities
Winter, Jennifer R. +11 more
core +1 more source
The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]
, 2005 During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V. +6 more
core +1 more source
DNA content of a functioning chicken kinetochore [PDF]
, 2014 In order to understand the three-dimensional structure of the functional kinetochore in vertebrates, we require a complete list and stoichiometry for the protein components of the kinetochore, which can be provided by genetic and proteomic experiments ...
Vagnarelli, Paola +5 more
core +1 more source
Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase. [PDF]
PLoS ONE, 2014 In the budding yeast, centromeres stay clustered near the spindle pole bodies (SPBs) through most of the cell cycle. This SPB-centromere proximity requires microtubules and functional kinetochores, which are protein complexes formed on the centromeres ...
Soumitra Sau +3 more
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Checkpoint proteins come under scrutiny [PDF]
, 2013 Details are emerging of the interactions between the kinetochore and various spindle checkpoint proteins that ensure that sister chromatids are equally divided between daughter cells during cell ...
Jonathan BA Millar +3 more
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PLoS Genetics, 2020 The kinetochore, a multi-protein complex assembled on centromeres, is essential to segregate chromosomes during cell division. Deficiencies in kinetochore function can lead to chromosomal instability and aneuploidy-a hallmark of cancer cells. Kinetochore
Guðjón Ólafsson, Peter H Thorpe
doaj +1 more source
The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells [PDF]
Chromosoma, 2006 Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells.
Maiato, H +6 more
openaire +3 more sources
Cdk1 inactivation terminates mitotic checkpoint surveillance and stabilizes kinetochore attachments in anaphase [PDF]
, 2014 Two mechanisms safeguard the bipolar attachment of chromosomes in mitosis. A correction mechanism destabilizes erroneous attachments that do not generate tension across sister kinetochores [1].
Vázquez-Novelle, María Dolores +6 more
core +1 more source
Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.
PLoS Genetics, 2016 The accurate segregation of chromosomes during cell division is achieved by attachment of chromosomes to the mitotic spindle via the kinetochore, a large multi-protein complex that assembles on centromeres.
Eva Herrero, Peter H Thorpe
doaj +1 more source