Results 31 to 40 of about 94,326 (306)

Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes [PDF]

, 2007
The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and generate diffusible anaphase inhibitors.
Rischitor, Patricia E, Kevin G Hardwick, Rischitor Patricia E., Patricia E Rischitor, May, Karen M, Hardwick Kevin G., Hardwick, Kevin G, Karen M May, May Karen M.   +8 more
core   +1 more source

Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore [PDF]

Open Biology, 2012
The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and
Zsolt Venkei, Marcin R. Przewloka, Yaseen Ladak, Shahad Albadri, Alex Sossick, Gabor Juhasz, Béla Novák, David M. Glover   +7 more
doaj   +1 more source

Visualizing kinetochore architecture [PDF]

Current Opinion in Structural Biology, 2011
Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo.
Gregory, Alushin, Eva, Nogales
openaire   +2 more sources

Kinetochore-localized PP1 prematurely silences the spindle checkpoint in meiosis.

, 2023
A) Representative time-lapse images of a SPC105WT cell completing both meiotic divisions. Time 0 indicates SPB separation. Scale bar = 5 μm. B) Graph showing the duration of metaphase I and metaphase II in SPC105WT cells with and without the anchor away ...
Soni Lacefield (573500), Victoria Vicory (15188303), Anne MacKenzie (15188300)   +2 more
core   +1 more source

Overexpression of spindle and kinetochore–associated protein 1 contributes to the progression of prostate cancer

Tumor Biology, 2017
Spindle and kinetochore–associated protein 1 is a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation.
Kai Wang, Ji Sun, Jingfei Teng, Yufu Yu, Dachuan Zhong, Yi Fan   +5 more
doaj   +1 more source

Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells

STAR Protocols, 2021
Summary: This protocol measures the 3D Euclidean distance (Δ3D) between two/three fluorescently labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.0.1, MATLAB based).
Tsvetelina E. Germanova, Emanuele Roscioli, Jonathan U. Harrison, Andrew D. McAinsh, Nigel J. Burroughs   +4 more
doaj   +1 more source

Integrating Functions at the Kinetochore [PDF]

Cell, 2002
Kinetochore components have catalytic as well as structural activities. New evidence illustrates how these proteins integrate spindle morphogenesis with regulation of the timing and accuracy of chromosome segregation.
Shimoda, Soni L., Solomon, Frank
openaire   +2 more sources

Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle

eLife, 2023
At each cell division, nanometer-scale components self-organize to build a micron-scale spindle. In mammalian spindles, microtubule bundles called kinetochore-fibers attach to chromosomes and focus into spindle poles.
Manuela Richter, Lila Neahring, Jinghui Tao, Renaldo Sutanto, Nathan H Cho, Sophie Dumont   +5 more
doaj   +1 more source

Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I [PDF]

, 2013
Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred
Sourav Sarkar, Prakash Arumugam, Arumugam P., Louise Newnham, Rajesh T Shenoy, Eva Hoffmann (159398), Sourav Sarkar (430861), Newnham, Louise, Millar J.B.A., Louise Newnham (430862), Shenoy, Rajesh T., Dalgaard J.Z., Prakash Arumugam (303310), Eva Hoffmann, Millar, Jonathan B.A., Newnham L., Sarkar, Sourav, Sarkar S., Millar, Jonathan B. A., Jacob Z. Dalgaard (303312), Arumugam, Prakash, Jonathan B. A. Millar (430863), Hoffmann, Eva, Jonathan B A Millar, Hoffmann E., Dalgaard, Jacob Z., Jacob Z Dalgaard, Rajesh T. Shenoy (223422), Shenoy R.T.   +28 more
core   +1 more source

Establishment of the vertebrate kinetochores [PDF]

Chromosome Research, 2012
The centromere is essential for accurate chromosome segregation during mitosis and meiosis to achieve transmission of genetic information to daughter cells. To facilitate accurate chromosome segregation, the centromere serves several specific functions, including microtubule binding, spindle-checkpoint control, and sister chromatid cohesion.
Tetsuya, Hori, Tatsuo, Fukagawa
openaire   +2 more sources