The path of DNA in the kinetochore [PDF]
Current Biology, 2006 The kinetochore is the protein-DNA complex at eukaryotic centromeres that functions as the attachment site for spindle microtubules. In budding yeast, the centromere spans 120 bp, there is a single microtubule per kinetochore, and the entire spindle is composed of 16 kinetochore microtubules plus four interpolar microtubules from each pole.
Shantanu Sharma +2 more
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Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I [PDF]
, 2013 Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred
A Petrovic +40 more
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Chromosome Segregation Is Biased by Kinetochore Size [PDF]
, 2018 Chromosome missegregation during mitosis or meiosis is a hallmark of cancer and the main cause of prenatal death in humans. The gain or loss of specific chromosomes is thought to be random, with cell viability being essentially determined by selection ...
Aguiar, P +8 more
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Reductionism at the vertebrate kinetochore [PDF]
Journal of Cell Biology, 2012 The kinetochore forms the site of attachment for mitotic spindle microtubules driving chromosome segregation. The interdependent protein interactions in this large structure have made it difficult to dissect the function of its components. In this issue, Hori et al. (2013. J. Cell Biol.
Stankovic, Ana, Jansen, Lars E.T.
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Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases [PDF]
, 2010 During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores,
Amaro, Ana C. +14 more
core +4 more sources
Balancing the kinetochore ledger [PDF]
Journal of Cell Biology, 2012 Reduction of polo-like kinase-1 (Plk1) at kinetochores as cells progress from prometaphase to metaphase is surprising given that the kinase is thought to stabilize kinetochore–microtubule (kt–MT) attachments. In this issue, Liu et al. (2012. J. Cell Biol.
Thomas J. Maresca, Stuart Cane
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Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]
, 2012 Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Bancroft, James M. +14 more
core +7 more sources
The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]
Open Biology, 2015 The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas +5 more
doaj +1 more source
Modeling Dual Pathways for the Metazoan Spindle Assembly Checkpoint [PDF]
, 2006 Using computational modelling, we investigate mechanisms of signal transduction focusing on the spindle assembly checkpoint where a single unattached kinetochore is able to signal to prevent cell cycle progression.
Acquaviva +29 more
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Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
Scientific Reports, 2017 The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat +3 more
doaj +1 more source