Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]
Open Biology, 2016 Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
doaj +1 more source
Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments [PDF]
, 2015 We previously reported that the spindle assembly checkpoint protein Bub3 is involved in regulating kinetochore-microtubule (KT-MT) attachments. Also, Bub3 was reported to interact with the microtubule motor protein dynein.
Barisic +27 more
core +1 more source
Modeling Dual Pathways for the Metazoan Spindle Assembly Checkpoint [PDF]
, 2006 Using computational modelling, we investigate mechanisms of signal transduction focusing on the spindle assembly checkpoint where a single unattached kinetochore is able to signal to prevent cell cycle progression.
Acquaviva +29 more
core +3 more sources
Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases [PDF]
, 2010 During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores,
Amaro, Ana C. +14 more
core +4 more sources
Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]
, 2012 Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Bancroft, James M. +14 more
core +7 more sources
Cell Reports, 2014 In many eukaryotes, the centromere is epigenetically specified and not strictly defined by sequence. In contrast, budding yeast has a specific 125 bp sequence required for kinetochore function.
Kung-Hsien Ho +3 more
doaj +1 more source
The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]
Open Biology, 2015 The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas +5 more
doaj +1 more source
Conformational mechanism for the stability of microtubule-kinetochore attachments [PDF]
, 2014 Regulating the stability of microtubule(MT)-kinetochore attachments is fundamental to avoiding mitotic errors and ensure proper chromosome segregation during cell division.
Bertalan, Zsolt +3 more
core +2 more sources
Adventures of the undead at kinetochores [PDF]
Molecular & Cellular Oncology, 2021 At the metaphase-to-anaphase transition, phosphatase activity feeds back to reverse early mitotic phosphorylation events. Our recent work indicates that the pseudokinase domain of the spindle checkpoint protein BUB1 (Budding Uninhibited by Benzimidazoles 1) mitotic checkpoint serine/threonine kinase B (BUB1B, BUBR1) maintains kinase-phosphatase balance
openaire +4 more sources
Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore
Scientific Reports, 2017 The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat +3 more
doaj +1 more source