Results 31 to 40 of about 40,318 (318)

Divergent polo box domains underpin the unique kinetoplastid kinetochore [PDF]

Open Biology, 2016
Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid ...
Olga O. Nerusheva, Bungo Akiyoshi
doaj   +1 more source

HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. [PDF]

, 2001
Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric mitotic and meiotic chromosomes of Caenorhabditis elegans ...
Albertson, Albertson, Albertson, Amon, Buchwitz, Dernburg, Fire, Gonczy, Harlow, Heald, Hedgecock, Hodgkin, Howman, Kainta, Kimble, Lombillo, Maney, McDonald, McEwen, Mello, Moore, Nabetani, Newman, Nicklas, O'Connell, Rappleye, Roos, Savoian, Shah, Sharp, Sharp, Wigge, Yanagida, Yao   +33 more
core   +2 more sources

Bod1, a novel kinetochore protein required for chromosome biorientation [PDF]

, 2007
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein
Andersen, Jens S., Blow, J. Julian, Hunter, Christopher J., Khoudoli, Guennadi A., McAinsh, Andrew D., McClelland, Sarah E., Porter, Iain M., Swedlow, Jason R.   +7 more
core   +3 more sources

A TOGgle for Tension at Kinetochores [PDF]

Cell, 2016
Differential stability of kinetochore-microtubule attachments at low versus high tension is critical for accurate chromosome segregation. Miller et al. find that a TOG domain microtubule-binding protein imparts intrinsic tension selectivity to kinetochore-microtubule attachments.
Bram Prevo, Bram Prevo, Dhanya K. Cheerambathur, Dhanya K. Cheerambathur, Arshad Desai, Arshad Desai   +5 more
openaire   +3 more sources

Localization and Function of Budding Yeast CENP-A Depends upon Kinetochore Protein Interactions and Is Independent of Canonical Centromere Sequence

Cell Reports, 2014
In many eukaryotes, the centromere is epigenetically specified and not strictly defined by sequence. In contrast, budding yeast has a specific 125 bp sequence required for kinetochore function.
Kung-Hsien Ho, Dai Tsuchiya, Audrey C. Oliger, Soni Lacefield   +3 more
doaj   +1 more source

CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly [PDF]

, 2016
Human centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alphoid DNA sequences. By inducing rapid, complete degradation of endogenous CENP-A, we now demonstrate that once the first steps of centromere ...
Barra V., Cleveland D. W., Dumont M., Fachinetti D., Herve S., Hoffmann S., Ly P., McMahon M. A., Nechemia-Arbely Y.   +8 more
core   +1 more source

Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.

PLoS Genetics, 2016
The accurate segregation of chromosomes during cell division is achieved by attachment of chromosomes to the mitotic spindle via the kinetochore, a large multi-protein complex that assembles on centromeres.
Eva Herrero, Peter H Thorpe
doaj   +1 more source

Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.

PLoS Genetics, 2020
The kinetochore, a multi-protein complex assembled on centromeres, is essential to segregate chromosomes during cell division. Deficiencies in kinetochore function can lead to chromosomal instability and aneuploidy-a hallmark of cancer cells. Kinetochore
Guðjón Ólafsson, Peter H Thorpe
doaj   +1 more source

Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore [PDF]

Open Biology, 2012
The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and
Zsolt Venkei, Marcin R. Przewloka, Yaseen Ladak, Shahad Albadri, Alex Sossick, Gabor Juhasz, Béla Novák, David M. Glover   +7 more
doaj   +1 more source

Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments [PDF]

, 2015
We previously reported that the spindle assembly checkpoint protein Bub3 is involved in regulating kinetochore-microtubule (KT-MT) attachments. Also, Bub3 was reported to interact with the microtubule motor protein dynein.
Barisic, Efimov, Gassmann, Gassmann, Gassmann, Hassan Bousbaa, Kops, Lo, Logarinho, Logarinho, Maia, Maresca, Musacchio, Patrícia M.A. Silva, Pfarr, Reis, Rieder, Sharp, Silva, Silva, Stehman, Steuer, Uchida, Vaisberg, Varma, Waters, Yang, Álvaro A. Tavares   +27 more
core   +1 more source