Results 41 to 50 of about 115,854 (373)

The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells [PDF]

Open Biology, 2015
The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle.
Gina V. Caldas, Tina R. Lynch, Ryan Anderson, Sana Afreen, Dileep Varma, Jennifer G. DeLuca   +5 more
doaj   +1 more source

Chromosome Oscillations in Mitosis [PDF]

, 2005
Successful cell division requires a tight regulation of chromosome motion via the activity of molecular motors. Many of the key players at the origin of the forces generating the movement have been identified, but their spatial and temporal organization ...
A. P. Joglekar, G. Östergren, J. C. Waters, L. Cassimeris, N. Tokai, N. G. Van Kampen, Otger Campàs, Pierre Sens, S. Inoue   +8 more
core   +2 more sources

Exotic mitotic mechanisms [PDF]

, 2012
The emergence of eukaryotes around two billion years ago provided new challenges for the chromosome segregation machineries: the physical separation of multiple large and linear chromosomes from the microtubule-organizing centres by the nuclear envelope.
Drechsler, Hauke, McAinsh, Andrew D.
core   +1 more source

Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.

PLoS Genetics, 2016
The accurate segregation of chromosomes during cell division is achieved by attachment of chromosomes to the mitotic spindle via the kinetochore, a large multi-protein complex that assembles on centromeres.
Eva Herrero, Peter H Thorpe
doaj   +1 more source

Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Scientific Reports, 2017
The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca2+ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the
Rinyaporn Phengchat, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui   +3 more
doaj   +1 more source

Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells

STAR Protocols, 2021
Summary: This protocol measures the 3D Euclidean distance (Δ3D) between two/three fluorescently labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.0.1, MATLAB based).
Tsvetelina E. Germanova, Emanuele Roscioli, Jonathan U. Harrison, Andrew D. McAinsh, Nigel J. Burroughs   +4 more
doaj   +1 more source

The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore

eLife, 2019
Kinetochores are macromolecular protein complexes at centromeres that ensure accurate chromosome segregation by attaching chromosomes to spindle microtubules and integrating safeguard mechanisms.
Josef Fischböck-Halwachs, Sylvia Singh, Mia Potočnjak, Götz Hagemann, Victor Solis-Mezarino, S. Woike, Medini Ghodgaonkar-Steger, Florian Weissmann, Laura D. Gallego, Julie Rojas, J. Andreani, A. Köhler, Franz Herzog   +12 more
semanticscholar   +1 more source

Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner. [PDF]

, 2019
The spindle assembly checkpoint (SAC) prevents anaphase until all kinetochores attach to the spindle. Each mammalian kinetochore binds many microtubules, but how many attached microtubules are required to turn off the checkpoint, and how the kinetochore ...
Akiyoshi, Alushin, Aravamudhan, Ballister, Barisic, Cane, Cheeseman, Chen, Ciferri, Clute, David, De Antoni, DeLuca, DeLuca, DeLuca, Dick, Dudka, Elting, Elting, Etemad, Etemad, Funabiki, Gassmann, Guimaraes, Hengeveld, Hewitt, Hiruma, Howell, Howell, Hueschen, Janssen, Jelluma, Ji, Johnston, Jonathan Kuhn, Kajtez, Khodjakov, Kuhn, London, Long, Lukinavičius, Maciejowski, Magidson, Maldonado, Maresca, Martin-Lluesma, Matson, McEwen, McIntosh, McKinley, Nijenhuis, Orth, O’Connell, Peterson, Pinsky, Rieder, Rieder, Santaguida, Sarangapani, Saurin, Shah, Sikirzhytski, Sikirzhytski, Sivakumar, Sophie Dumont, Sundin, Suzuki, Suzuki, Tanaka, Tauchman, Uchida, Uetake, Vanoosthuyse, Volkov, Wan, Waters, Wendell, Wojcik, Yoo, Zaytsev, Zaytsev   +80 more
core   +1 more source

Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA.

PLoS Genetics, 2020
The kinetochore, a multi-protein complex assembled on centromeres, is essential to segregate chromosomes during cell division. Deficiencies in kinetochore function can lead to chromosomal instability and aneuploidy-a hallmark of cancer cells. Kinetochore
Guðjón Ólafsson, Peter H Thorpe
doaj   +1 more source

Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase. [PDF]

PLoS ONE, 2014
In the budding yeast, centromeres stay clustered near the spindle pole bodies (SPBs) through most of the cell cycle. This SPB-centromere proximity requires microtubules and functional kinetochores, which are protein complexes formed on the centromeres ...
Soumitra Sau, Sabyasachi Sutradhar, Raja Paul, Pratima Sinha   +3 more
doaj   +1 more source