Results 11 to 20 of about 54,550 (246)
Replacement recombination in Lactococcus lactis [PDF]
In the pUC18-derived integration plasmid pML336 there is a 5.3-kb chromosomal DNA fragment that carries the X-prolyl dipeptidyl aminopeptidase gene (pepXP). The gene was inactivated by the insertion of an erythromycin resistance determinant into its coding sequence.
Leenhouts, Cornelis +2 more
openaire +3 more sources
The Lactococcus lactis Pan-Plasmidome [PDF]
Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival.
Philip Kelleher +8 more
openaire +5 more sources
Gene expression in Lactococcus lactis [PDF]
Lactic acid bacteria are of major economic importance, as they occupy a key position in the manufacture of fermented foods. A considerable body of research is currently being devoted to the development of lactic acid bacterial strains with improved characteristics, that may be used to make fermentations pass of more efficiently, or to make new ...
Guchte, Maarten van de +2 more
openaire +3 more sources
Functionality of Sortase A in Lactococcus lactis [PDF]
ABSTRACT Lactococcus lactis IL1403 harbors a putative sortase A (SrtA) and 11 putative sortase substrates that carry the canonical LPXTG signature of such substrates. We report here on the functionality of SrtA to anchor five LPXTG substrates to the cell wall, thus suggesting that SrtA is the housekeeping sortase in
Dieye, Yakhya +7 more
openaire +4 more sources
Background Over the last two decades, several cases of infections caused by Lactococcus lactis have been reported. This Gram-positive coccus is considered non-pathogenic for humans.
Wahib Lahlou +6 more
doaj +1 more source
CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis [PDF]
Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-
Cano Garrido, Olivia +7 more
core +2 more sources
Tryptophan biosynthesis genes in Lactococcus lactis subsp. lactis [PDF]
The Lactococcus lactis chromosomal region containing the seven structural genes required for tryptophan biosynthesis was characterized by cloning and sequencing. All of the trp genes were identified by the homology of their products with known Trp proteins from other organisms.
Bardowski, J. +2 more
openaire +3 more sources
Functional analysis of the Lactococcus lactis galU and galE genes and their impact on sugar nucleotide and exopolysaccharide biosynthesis [PDF]
We studied the UDP-glucose pyrophosphorylase (galU) and UDP-galactose epimerase (galE) genes of Lactococcus lactis MG1363 to investigate their involvement in biosynthesis of UDP-glucose and UDP-galactose, which are precursors of glucose- and galactose ...
Boels, I.C. +3 more
core +1 more source
Relationship between glycolysis and exopolysaccharide biosynhesis in Lactococcus lactis [PDF]
The relationships between glucose metabolism and exopolysaccharide (EPS) production in a Lactococcus lactis strain containing the EPS gene cluster (Eps ) and in nonproducer strain MG5267 (Eps) were characterized.
Ramos, A. +3 more
core +1 more source
Isolation and characterization of Lactococcus lactis subsp. lactis promoters [PDF]
DNA fragments with promoter activity were isolated from the chromosome of Lactococcus lactis subsp. lactis. For the isolation, a promoter probe vector based on the cat gene was constructed, which allowed direct selection with chloramphenicol in Bacillus subtilis and L. lactis.
T, Koivula, M, Sibakov, I, Palva
openaire +2 more sources

