Results 31 to 40 of about 23,804 (222)

The large fraction of heterochromatin in Drosophila neurons is bound by both B-type lamin and HP1a

Epigenetics & Chromatin, 2018
Background In most mammalian cell lines, chromatin located at the nuclear periphery is represented by condensed heterochromatin, as evidenced by microscopy observations and DamID mapping of lamina-associated domains (LADs) enriched in dimethylated Lys9 ...
Alexey V. Pindyurin, Artem A. Ilyin, Anton V. Ivankin, Mikhail V. Tselebrovsky, Valentina V. Nenasheva, Elena A. Mikhaleva, Ludo Pagie, Bas van Steensel, Yuri Y. Shevelyov   +8 more
doaj   +1 more source

A Lamina-Associated Domain Border Governs Nuclear Lamina Interactions, Transcription, and Recombination of the Tcrb Locus [PDF]

Cell Reports, 2018
Tcrb locus V(D)J recombination is regulated by positioning at the nuclear periphery. Here, we used DamID to profile Tcrb locus interactions with the nuclear lamina at high resolution. We identified a lamina-associated domain (LAD) border composed of several CTCF-binding elements that segregates active non-LAD from inactive LAD regions of the locus ...
Shiwei Chen, Teresa Romeo Luperchio, Xianrong Wong, Europe B. Doan, Aaron T. Byrd, Kingshuk Roy Choudhury, Karen L. Reddy, Michael S. Krangel   +7 more
openaire   +3 more sources

Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation

Cell Reports, 2020
Summary: Repetitive elements are abundantly distributed in mammalian genomes. Here, we reveal a striking association between repeat subtypes and gene function. SINE, L1, and low-complexity repeats demarcate distinct functional categories of genes and may
J. Yuyang Lu, Wen Shao, Lei Chang, Yafei Yin, Tong Li, Hui Zhang, Yantao Hong, Michelle Percharde, Lerui Guo, Zhongyang Wu, Lichao Liu, Wei Liu, Pixi Yan, Miguel Ramalho-Santos, Yujie Sun, Xiaohua Shen   +15 more
doaj   +1 more source

Promoter-intrinsic and local chromatin features determine gene repression in lamina-associated domains [PDF]

, 2018
Abstract It is largely unclear whether genes that are naturally embedded in lamina associated domains (LADs) are inactive due to their chromatin environment, or whether LADs are merely secondary to the lack of transcription. We show that hundreds of human promoters become active when moved from their native LAD position to a neutral ...
Leemans, Christ, van der Zwalm, Marloes, Brueckner, Laura, Comoglio, Federico, van Schaik, Tom, Pagie, Ludo, van Arensbergen, Joris, van Steensel, Bas   +7 more
openaire   +2 more sources

MAN1, an Inner Nuclear Membrane Protein That Shares the LEM Domain with Lamina-associated Polypeptide 2 and Emerin [PDF]

Journal of Biological Chemistry, 2000
The "MAN antigens" are polypeptides recognized by autoantibodies from a patient with a collagen vascular disease and localized to the nuclear envelope. We now show that one of the human MAN antigens termed MAN1 is a 82.3-kDa protein with an amino-terminal domain followed by two hydrophobic segments and a carboxyl-terminal tail.
F, Lin, D L, Blake, I, Callebaut, I S, Skerjanc, L, Holmer, M W, McBurney, M, Paulin-Levasseur, H J, Worman   +7 more
openaire   +2 more sources

Role of histone deacetylases in gene regulation at nuclear lamina.

PLoS ONE, 2012
Theoretical models suggest that gene silencing at the nuclear periphery may involve "closing" of chromatin by transcriptional repressors, such as histone deacetylases (HDACs). Here we provide experimental evidence confirming these predictions.
Beatrice C Milon, Haibo Cheng, Mikhail V Tselebrovsky, Sergei A Lavrov, Valentina V Nenasheva, Elena A Mikhaleva, Yuri Y Shevelyov, Dmitry I Nurminsky   +7 more
doaj   +1 more source

Hmga2 protein loss alters nuclear envelope and 3D chromatin structure

BMC Biology, 2022
Background The high-mobility group Hmga family of proteins are non-histone chromatin-interacting proteins which have been associated with a number of nuclear functions, including heterochromatin formation, replication, recombination, DNA repair ...
Giuseppina Divisato, Andrea M. Chiariello, Andrea Esposito, Pietro Zoppoli, Federico Zambelli, Maria Antonietta Elia, Graziano Pesole, Danny Incarnato, Fabiana Passaro, Silvia Piscitelli, Salvatore Oliviero, Mario Nicodemi, Silvia Parisi, Tommaso Russo   +13 more
doaj   +1 more source

Hotspots of De Novo Point Mutations in Induced Pluripotent Stem Cells

Cell Reports, 2017
Summary: Induced pluripotent stem cells (iPSCs) are generated by direct reprogramming of somatic cells and hold great promise for novel therapies. However, several studies have reported genetic variations in iPSC genomes.
Masahito Yoshihara, Ryoko Araki, Yasuji Kasama, Misato Sunayama, Masumi Abe, Kohji Nishida, Hideya Kawaji, Yoshihide Hayashizaki, Yasuhiro Murakawa   +8 more
doaj   +1 more source

Role of lamins in 3D genome organization and global gene expression

Nucleus, 2019
Genome-wide mapping of lamin-B1-genome interactions has shown that gene-poor and transcriptionally inactive genomic regions are associated with the nuclear lamina.
Youngjo Kim, Xiaobin Zheng, Yixian Zheng
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source