Results 51 to 60 of about 14,738 (282)

Mechanoregulatory Effects of Cell‐Scale Microwells on Epithelial Cell Phenotype

Advanced Functional Materials, EarlyView.
In small polycaprolactone microwells, A549 epithelial cells span well edges, in contrast to cells growing on flat substrates. Focal adhesion sites (yellow) concentrate at topographic boundaries, while cytoskeletal tension (magenta stress fibers) is transmitted to the nucleus (blue), reducing nuclear sphericity.
Ruiwen He, Wang Sik Lee, Phattadon Yajan, Aaron Lee, Mauro Sousa de Almeida, Sandeep Keshavan, Jozef Adamcik, Céline Loussert‐Fonta, Dimitri Vanhecke, Barbara Rothen‐Rutishauser, Alke Petri‐Fink   +10 more
wiley   +1 more source

Need for NAD⁺: Focus on Striated Muscle Laminopathies

Cells, 2020
Laminopathies are a heterogeneous group of rare diseases caused by genetic mutations in the LMNA gene, encoding A-type lamins. A-type lamins are nuclear envelope proteins which associate with B-type lamins to form the nuclear lamina, a meshwork ...
Déborah Cardoso, Antoine Muchir
doaj   +1 more source

Nuclear envelope, chromatin organizers, histones, and DNA: The many achilles heels exploited across cancers

Frontiers in Cell and Developmental Biology, 2022
In eukaryotic cells, the genome is organized in the form of chromatin composed of DNA and histones that organize and regulate gene expression. The dysregulation of chromatin remodeling, including the aberrant incorporation of histone variants and their ...
A. K. Balaji, Santam Saha, Shruti Deshpande, Darshini Poola, Kundan Sengupta   +4 more
doaj   +1 more source

Sheet lamination

, 2022
The sheet lamination (SHL) is one of the processes in additive technologies. In the past, it has been used extensively to make fast, inexpensive paper prototypes. Then the application expanded to metals, polymers (only thermoplastics), textiles and ceramics, which also required the improvement of procedures, i.e. devices (3D printers).
openaire   +2 more sources

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Weaving a pattern from disparate threads: lamin function in nuclear assembly and DNA replication [PDF]

, 1994
The major residual structure that remains associated with the nuclear envelope following extraction of isolated nuclei or oocyte germinal vesicles with non-ionic detergents, nucleases and high salt is the lamina (Fawcett, 1966; Aaronson and Blobel ...
Bridger, JM, Cox, LS, Hutchison, CJ, Kill, IR   +3 more
core  

Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex

IBRO Reports, 2018
Lamins are type V intermediate filament proteins that are located beneath the inner nuclear membrane. In mammalian somatic cells, LMNB1 and LMNB2 encode somatic lamins B1 and B2, respectively, and the LMNA gene is alternatively spliced to generate ...
Yasuharu Takamori, Yukie Hirahara, Taketoshi Wakabayashi, Tetsuji Mori, Taro Koike, Yosky Kataoka, Yasuhisa Tamura, Shuji Kurebayashi, Kiyoshi Kurokawa, Hisao Yamada   +9 more
doaj   +1 more source

The Floor‐Ceiling‐Chip, or 2 × 2D = Pseudo‐3D—Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell‐Adhesive Protein Micropatterns

Advanced Healthcare Materials, EarlyView.
Here, we present a novel 3D cell patterning and culture platform. The “Floor‐Ceiling‐Chip” (FC‐Chip) consists of two opposing track‐etched membranes, creating a pseudo‐3D microenvironment for the cells in between. By providing the membranes with micropatterned cell‐adhesive islands of varying geometries and sizes, the FC‐Chip enables control over cell ...
Urandelger Tuvshindorj, Francesca Giacomini, Esra Güben Kaçmaz, Tim ten Brink, Lorenzo Moroni, Zeinab Tahmasebi Birgani, Clemens van Blitterswijk, Pamela Habibović, Stefan Giselbrecht, Jan de Boer, Roman Truckenmüller   +10 more
wiley   +1 more source

The Nuclear Envelope as a Regulator of Immune Cell Function

Frontiers in Immunology, 2022
The traditional view of the nuclear envelope (NE) was that it represented a relatively inert physical barrier within the cell, whose main purpose was to separate the nucleoplasm from the cytoplasm.
Anna Selezneva, Alasdair J. Gibb, Dean Willis   +2 more
doaj   +1 more source

Common Signatures of Altered Gene Regulation and Invasiveness of Different Breast Cancer Cell Lines after Matrix Interface Crossing

Advanced Healthcare Materials, EarlyView.
Interface transmigration reprograms triple‐negative breast cancer cells, triggering a shared switch toward more aggressive and invasive phenotypes. Using a collagen I interface model, this study identifies shared transcriptional changes involving proliferation, chromatin remodeling, and DNA repair pathways.
Cornelia Clemens, Hannah Trampert, Nataliia Kotsiuba, Tilo Pompe   +3 more
wiley   +1 more source