Results 181 to 190 of about 2,855 (207)

Determination of serum levels of unesterified lathosterol by isotope dilution-mass spectrometry

Scandinavian Journal of Clinical and Laboratory Investigation, 1989
The synthesis of 2H3-labelled lathosterol is described. This compound was used together with 2H7-labelled cholesterol for simultaneous assay of unesterified lathosterol and cholesterol in serum by isotope dilution-mass spectrometry. After addition of a fixed amount of the two internal standards to a fixed amount of serum (in general 25 microliter), the
E, Lund, L, Sisfontes, E, Reihner, I, Bjorkhem   +3 more
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Biliary Lathosterol and Other Cholesterol Precursor Sterols Are Increased in Patients with Ileal Exclusion

Scandinavian Journal of Gastroenterology, 1988
The human bile contains several noncholesterol sterols, of which the cholesterol precursor sterols are quantitatively the most important. Detailed data on factors that regulate the amount of these sterols in the bile have not been available. In this study the effect of chronic stimulation of cholesterol synthesis on biliary cholesterol precursor sterol
P V, Koivisto, T A, Miettinen
openaire   +2 more sources

Biological variation of β-sitosterol, campesterol, and lathosterol as cholesterol absorption and synthesis biomarkers

Clinica Chimica Acta, 2014
The analysis of blood for β-sitosterol and campesterol is the measures of cholesterol absorption while lathosterol is a measure of cholesterol synthesis.The biological variability of β-sitosterol, campesterol, and lathosterol was measured using liquid-chromatography tandem mass spectrometry from a cohort of 25 apparently healthy subjects, where blood ...
Alan H B, Wu, Weiming, Ruan, John, Todd, Kara L, Lynch   +3 more
openaire   +2 more sources

Ratio of lathosterol to campesterol in serum predicts the cholesterol-lowering effect of sitostanol-supplemented margarine

Int. Journal of Clinical Pharmacology and Therapeutics, 2005
Plant sterol/stanol margarines are recommended as a lipid-lowering dietary supplement in the treatment of hypercholesterolemia. Parameters predicting the individual cholesterol-lowering effect have not been elucidated so far. Therefore, we investigated the responsiveness to sitostanol-supplemented margarine in a specially selected population.From a ...
S C, Thuluva, M, Igel, U, Giesa, D, Lütjohann, T, Sudhop, K, von Bergmann   +5 more
openaire   +2 more sources

Effects of dietary coconut oil, butter and safflower oil on plasma lipids, lipoproteins and lathosterol levels

European Journal of Clinical Nutrition, 1998
The aim of this present study was to determine plasma levels of lathosterol, lipids, lipoproteins and apolipoproteins during diets rich in butter, coconut fat and safflower oil.The study consisted of sequential six week periods of diets rich in butter, coconut fat then safflower oil and measurements were made at baseline and at week 4 in each diet ...
C, Cox, W, Sutherland, J, Mann, S, de Jong, A, Chisholm, M, Skeaff   +5 more
openaire   +2 more sources

Gas chromatographic–mass spectrometric determination of brain levels of α-cholest-8-en-3β-ol (lathosterol)

Journal of Chromatography B, 2007
A gas chromatographic-mass spectrometric (GC-MS) method is proposed for the detection and quantification of lathosterol in rabbit brain. This compound is one of the most important precursors of the cholesterol synthesis. The interest in brain cholesterol metabolism is growing nowadays since it was described to play an important role in some ...
Berta, Luzón-Toro, Alberto, Zafra-Gómez, Oscar, Ballesteros   +2 more
openaire   +2 more sources

Partial purification and characterization of lathosterol 5-desaturase from rat liver microsomes.

Journal of biochemistry, 1985
The terminal oxidase of the NADH-dependent lathosterol 5-desaturation system was solubilized from rat liver microsomes with 2% Triton X-100, and partially purified approximately 18-fold with 19% yield after DEAE-cellulose and 6-aminohexyl-Sepharose column chromatography.
K, Honjo, T, Ishibashi, Y, Imai
openaire   +1 more source

Simultaneous determination of serum lathosterol and cholesterol by semi-micro high-performance liquid chromatography with electrochemical detection

Journal of Chromatography B, 2011
A simple method has been developed for the simultaneous determination of lathosterol and cholesterol by high-performance liquid chromatography with electrochemical detection (HPLC-ECD). Lathosterol was found to be electrochemically oxidized and its current peak height was linearly related to the amount of lathosterol injected, ranging from 0.15 μmol/L ...
Kazuhiro, Hojo, Hideki, Hakamata, Fumiyo, Kusu   +2 more
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Low serum lathosterol levels associate with fatal cardiovascular disease and excess all-cause mortality: a prospective cohort study

Clinical Research in Cardiology, 2019
A more precise identification of patients at "high cardiovascular risk" is preeminent in cardiovascular risk stratification.To investigate the relationships between markers of cholesterol homeostasis, cardiovascular events and all-cause mortality.We quantified markers of cholesterol homeostasis by gas chromatography-mass spectrometry in 377 subjects ...
Weingärtner, O, Lütjohann, D, Meyer, S, Fuhrmann, A, Cremers, B, Seiler-Mußler, S, Schött, HF, Kerksiek, A, Friedichs, S, Ulbricht, N, Zawada, A, Laufs, U, Schulze, PC, Scheller, B, Fliser, D, Böhm, M, Sijbrands, E.J.G., Heine, G   +17 more
openaire   +3 more sources

Lathosterol to cholesterol ratios in cystic fibrosis

Gastroenterology, 1998
J.C. Deutsch, M. Czopek, J. Bisping, D. Rodman, J.F. Kolhouse   +4 more
openaire   +1 more source