Identification of a small molecule for enhancing lentiviral transduction of T cells [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 Genetic modification of cells using viral vectors has shown huge therapeutic benefit in multiple diseases. However, inefficient transduction contributes to the high cost of these therapies.
Paulina Malach +10 more
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CD9 and folate receptor overexpression are not sufficient for VSV-G-independent lentiviral transduction. [PDF]
PLoS ONE, 2022 Extracellular vesicles have become a research focus for their potential as therapeutic vehicles that carry cargo substances. Extracellular vesicles may origin from the endosomal compartment and share several characteristics with the envelope of ...
Cristina Bellotti +2 more
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Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion [PDF]
Molecular Therapy: Methods & Clinical Development, 2021 Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large ...
David S.J. Allan +6 more
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Hypoxia and HIF-1 inhibition enhance lentiviral transduction efficiency: a novel strategy for gene delivery optimization [PDF]
BMC BiotechnologyLentiviral vectors are widely used for stable gene delivery, but their transduction efficiency can be limited by suboptimal experimental conditions. Here, we investigated the role of oxygen concentration and hypoxia-inducible factor 1 (HIF-1) signaling ...
Qianyu Huo +10 more
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High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2 [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies.
Naoya Uchida +9 more
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Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors [PDF]
Journal of Biological Engineering, 2009 Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
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A Nontoxic Transduction Enhancer Enables Highly Efficient Lentiviral Transduction of Primary Murine T Cells and Hematopoietic Stem Cells [PDF]
Molecular Therapy: Methods & Clinical Development, 2018 Lentiviral vectors have emerged as an efficient, safe therapeutic tool for gene therapy based on hematopoietic stem cells (HSCs) or T cells. However, the monitoring of transduced cells in preclinical models remains challenging because of the inefficient ...
Marianne Delville +8 more
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The effect of enhancers on the lentiviral transduction efficiency in the human RPE cells: Insights for advancing retinal gene therapies [PDF]
Biochemistry and Biophysics ReportsBackground: Viral vectors including lentiviruses (LV), adenoviruses (AV) and adeno-associated viruses (AAV) have been used as common vehicles for gene transfer in gene therapy of various human diseases.
Sajad Najafi +6 more
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Lentiviral transduction of neuronal cells. [PDF]
Methods Mol Biol, 2013 Here we describe a general method for the construction of a lentivirus vector using a specific example of the construction of a lentivirus containing the luciferase reporter gene under the control of two hypothetical promoters and derived HIV-1 based lentivirus expression vector pLVX-Puro.
Wollebo HS, Woldemichaele B, White MK.
europepmc +4 more sources
Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction [PDF]
Viruses, 2020 Lentiviral vectors are versatile tools for gene delivery purposes. While in the earlier versions of retroviral vectors, transgene expression was controlled by the long terminal repeats (LTRs), the latter generations of vectors, including those derived ...
Audrey Page +2 more
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