Results 11 to 20 of about 115,363 (220)

Optimized protocols for γδ T cell expansion and lentiviral transduction. [PDF]

Mol Med Rep, 2019
γδ T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically‑modified γδ T cells is regarded as a promising tool for tumor treatment. However, since γδ T cells constitute a minority of T cells, their large‑scale expansion is difficult to realize in an ...
Wang RN, Wen Q, He WT, Yang JH, Zhou CY, Xiong WJ, Ma L.   +6 more
europepmc   +4 more sources

A protocol for lentiviral transduction and downstream analysis of intestinal organoids. [PDF]

J Vis Exp, 2015
Intestinal crypt-villus structures termed organoids, can be kept in sustained culture three dimensionally when supplemented with the appropriate growth factors. Since organoids are highly similar to the original tissue in terms of homeostatic stem cell differentiation, cell polarity and presence of all terminally differentiated cell types known to the ...
Van Lidth de Jeude JF, Vermeulen JL, Montenegro-Miranda PS, Van den Brink GR, Heijmans J.   +4 more
europepmc   +4 more sources

DEAE-Dextran Enhances the Lentiviral Transduction of Primary Human Mesenchymal Stromal Cells from All Major Tissue Sources Without Affecting Their Proliferation and Phenotype. [PDF]

Mol Biotechnol, 2023
Amadeo F, Hanson V, Murray P, Taylor A.
europepmc   +3 more sources

Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells. [PDF]

PLoS ONE, 2015
The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes ...
Wojciech Witkowski, Jolien Vermeire, Alessia Landi, Evelien Naessens, Hanne Vanderstraeten, Hans Nauwynck, Herman Favoreel, Bruno Verhasselt   +7 more
doaj   +6 more sources

CD20 and CD19 targeted vectors induce minimal activation of resting B lymphocytes [PDF]

, 2013
B lymphocytes are an important cell population of the immune system. However, until recently it was not possible to transduce resting B lymphocytes with retro- or lentiviral vectors, making them unsusceptible for genetic manipulations by these vectors ...
A Pezzutto, A Yoder, AD Bucheit, B Anliker, C Demaison, C Frecha, C Frecha, C Frecha, CA Walshe, Christian J. Buchholz, CJ Buchholz, D Unutmaz, DA Tuveson, DD Sloan, Els Verhoeyen, Evren Alici, François-Loic Cosset, H Li, H Niiro, J Bruenke, J Uchida, JT Golay, K Akashi, K Itoh, L Ginaldi, M Fujimoto, M Kügler, M Peipp, M Serafini, M Zanetti, MD Mühlebach, Michael Schwenkert, MJ Polyak, MS Cragg, NE Harwood, Q Zhou, Q Zhou, Q Zhou, Qi Zhou, R Zufferey, RC Münch, RN Damle, RS Noyce, S Funke, S Kheirallah, S Kneissl, Sabrina Kneissl, T Nakamura, TF Tedder, X Li, Y Reiter, YD Korin   +51 more
core   +23 more sources

Combined effects of restriction factors and transduction adjuvants on lentiviral vector gene transfer efficacy [PDF]

Scientific Reports
Restriction factors include various cellular proteins that detect and impede viral infections. Among them, interferon-induced transmembrane (IFITM) and serine incorporator (SERINC) proteins interfere with the infectious cycle of HIV-1. Consequently, such
Marie Dewannieux, Fatoumata Fofana, Kathleen Hamon, Mirella Mormin, Anne Galy   +4 more
doaj   +2 more sources

Purification of Human CD34⁺CD90⁺ HSCs Reduces Target Cell Population and Improves Lentiviral Transduction for Gene Therapy. [PDF]

Mol Ther Methods Clin Dev, 2020
Radtke S, Pande D, Cui M, Perez AM, Chan YY, Enstrom M, Schmuck S, Berger A, Eunson T, Adair JE, Kiem HP.   +10 more
europepmc   +3 more sources

Process for an efficient lentiviral cell transduction [PDF]

Biology Methods and Protocols, 2020
Abstract The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause ...
Anna Chiara Pirona, Risky Oktriani, Michael Boettcher, Jörg D Hoheisel   +3 more
openaire   +2 more sources

Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795

Journal of Translational Medicine, 2020
Background Adoptive transfer of engineered immune cells is a promising strategy for cancer treatment. However, low transduction efficiency particularly when large payload lentiviral vectors are used on primary T cells is a limitation for the development ...
Lingyu Li, Yuan Gao, Richa Srivastava, Wei Wang, Qinghui Xiong, Zhiming Fang, Alejandra Pelayo, Carolyn Denson, Angshumala Goswami, Rona Harari-Steinfeld, Zhifen Yang, Lihong Weng, Lei Stanley Qi, Francesco M. Marincola   +13 more
doaj   +1 more source

Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]

, 2014
Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R, Gschweng, Eric H, Kohn, Donald B, Lill, Georgia R   +3 more
core   +1 more source