Lentiviral transduction of neuronal cells. [PDF]
Methods Mol Biol, 2013 Here we describe a general method for the construction of a lentivirus vector using a specific example of the construction of a lentivirus containing the luciferase reporter gene under the control of two hypothetical promoters and derived HIV-1 based lentivirus expression vector pLVX-Puro.
Wollebo HS, Woldemichaele B, White MK.
europepmc +4 more sources
Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells. [PDF]
PLoS ONE, 2016 Diffuse large B-cell lymphoma (DLBCL) is characterized by great genetic and clinical heterogeneity which complicates prognostic prediction and influences treatment efficacy. The most common regimen, R-CHOP, consists of a combination of anthracycline- and
Benyamin Ranjbar +6 more
doaj +3 more sources
Antibody‐Based CAR T Cells Produced by Lentiviral Transduction [PDF]
Current Protocols in Immunology, 2020 AbstractOne promising approach to treat hematologic malignancies is the usage of patient‐derived CAR T cells. There are continuous efforts to improve the function of these cells, to optimize their receptor, and to use them for the treatment of additional types of cancer and especially solid tumors.
Sabrina Prommersberger +2 more
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Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells. [PDF]
PLoS ONE, 2015 The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes ...
Wojciech Witkowski +7 more
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Engineering single-vector logic-gated CAR T cells with transgene sizes beyond current limitations [PDF]
Journal for ImmunoTherapy of CancerBackground Engineering chimeric antigen receptor (CAR) T cells with logic-gated synthetic Notch (synNotch) receptor circuits can enhance specificity and mitigate on-target/off-tumor toxicity.
Carl H June +10 more
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Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction [PDF]
Viruses, 2020 Lentiviral vectors are versatile tools for gene delivery purposes. While in the earlier versions of retroviral vectors, transgene expression was controlled by the long terminal repeats (LTRs), the latter generations of vectors, including those derived ...
Audrey Page +2 more
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Lentiviral transduction v1 [PDF]
Lentiviral transduction of primary neurons
Bishal Basak, Erika L.F. Holzbaur
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Analysis of Hepatic Lentiviral Vector Transduction: Implications for Preclinical Studies and Clinical Gene Therapy Protocols [PDF]
VirusesLentiviral vector-transduced T cells were approved by the FDA as gene therapy anti-cancer medications. Little is known about the effects of host genetic variation on the safety and efficacy of the lentiviral vector gene delivery system.
Peirong Hu +5 more
doaj +2 more sources
DEAE-Dextran Enhances the Lentiviral Transduction of Primary Human Mesenchymal Stromal Cells from All Major Tissue Sources Without Affecting Their Proliferation and Phenotype. [PDF]
Mol Biotechnol, 2023 Amadeo F, Hanson V, Murray P, Taylor A.
europepmc +3 more sources
Measles virus glycoprotein-based lentiviral targeting vectors that avoid neutralizing antibodies [PDF]
, 2012 Lentiviral vectors (LVs) are potent gene transfer vehicles frequently applied in research and recently also in clinical trials. Retargeting LV entry to cell types of interest is a key issue to improve gene transfer safety and efficacy.
Abel, Tobias +5 more
core +23 more sources