Modular Lentiviral Vectors for Highly Efficient Transgene Expression in Resting Immune Cells
Viruses, 2021 Gene/cell therapies are promising strategies for the many presently incurable diseases. A key step in this process is the efficient delivery of genes and gene-editing enzymes to many cell types that may be resistant to lentiviral vector transduction ...
Christina Fichter +11 more
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A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers. [PDF]
, 2019 Cohesin is a multiprotein ring that is responsible for cohesion of sister chromatids and formation of DNA loops to regulate gene expression. Genomic analyses have identified that the cohesin subunit STAG2 is frequently inactivated by mutations in cancer.
Ashworth, Alan +4 more
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Gene delivery to pancreatic exocrine cells
BMC Biotechnology, 2012 Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle +4 more
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Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector. [PDF]
, 2010 One of the major hurdles for the development of gene therapy for Fanconi anemia (FA) is the increased sensitivity of FA stem cells to free radical-induced DNA damage during ex vivo culture and manipulation.
Adair, J +10 more
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An efficient method for the transduction of primary pediatric glioma neurospheres
MethodsX, 2018 Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel +4 more
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Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells. [PDF]
PLoS ONE, 2016 Diffuse large B-cell lymphoma (DLBCL) is characterized by great genetic and clinical heterogeneity which complicates prognostic prediction and influences treatment efficacy. The most common regimen, R-CHOP, consists of a combination of anthracycline- and
Benyamin Ranjbar +6 more
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Driving DNA transposition by lentiviral protein transduction [PDF]
Mobile Genetic Elements, 2014 Gene vectors derived from DNA transposable elements have become powerful molecular tools in biomedical research and are slowly moving into the clinic as carriers of therapeutic genes. Conventional uses of DNA transposon-based gene vehicles rely on the intracellular production of the transposase protein from transfected nucleic acids.
Cai, Yujia, Mikkelsen, Jacob Giehm
openaire +2 more sources
Molecular Therapy: Methods & Clinical Development, 2016 Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy +4 more
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Expression of a large coding sequence: Gene therapy vectors for Ataxia Telangiectasia
Scientific Reports, 2023 Ataxia telangiectasia is a monogenetic disorder caused by mutations in the ATM gene. Its encoded protein kinase ATM plays a fundamental role in DNA repair of double strand breaks (DSBs).
Tanja Hirch +6 more
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Immunogenicity of targeted lentivectors [PDF]
, 2014 To increase the safety and possibly efficacy of HIV-1 derived lentivectors (LVs) as an anti-cancer vaccine, we recently developed the Nanobody (Nb) display technology to target LVs to antigen presenting cells (APCs).
Adotévi +67 more
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