Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications.
PLoS ONE, 2012 Epigenetic modifications may occur during in vitro manipulations of stem cells but these effects have remained unexplored in the context of cell and gene therapy protocols.
Yoshiaki Yamagata +10 more
doaj +5 more sources
An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo. [PDF]
J Anim Sci Biotechnol, 2015 Qin J +7 more
europepmc +3 more sources
Process for an efficient lentiviral cell transduction [PDF]
Biology Methods and Protocols, 2020 Abstract The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause ...
Anna Chiara Pirona +3 more
openaire +2 more sources
Avoiding lentiviral transduction culture induced MSC senescence [PDF]
Journal of Cellular and Molecular Medicine, 2009 Dear Editor: We read with interest the recent article by Bocker and colleagues in JCMM[1]. As noted by the authors, mesenchymal stem cells (MSC) enjoy considerable therapeutic potential in regenerative medicine, cell therapy for select genetic diseases and as immunomodulators [2, 3].
Pan, Yung-Wei, Kurre, Peter
openaire +3 more sources
Journal of Translational Medicine, 2020 Background Adoptive transfer of engineered immune cells is a promising strategy for cancer treatment. However, low transduction efficiency particularly when large payload lentiviral vectors are used on primary T cells is a limitation for the development ...
Lingyu Li +13 more
doaj +1 more source
Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector. [PDF]
, 2010 One of the major hurdles for the development of gene therapy for Fanconi anemia (FA) is the increased sensitivity of FA stem cells to free radical-induced DNA damage during ex vivo culture and manipulation.
Adair, J +10 more
core +2 more sources
Driving DNA transposition by lentiviral protein transduction [PDF]
Mobile Genetic Elements, 2014 Gene vectors derived from DNA transposable elements have become powerful molecular tools in biomedical research and are slowly moving into the clinic as carriers of therapeutic genes. Conventional uses of DNA transposon-based gene vehicles rely on the intracellular production of the transposase protein from transfected nucleic acids.
Cai, Yujia, Mikkelsen, Jacob Giehm
openaire +2 more sources
Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
core +1 more source
Gene delivery to pancreatic exocrine cells
BMC Biotechnology, 2012 Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle +4 more
doaj +1 more source
A requirement for STAG2 in replication fork progression creates a targetable synthetic lethality in cohesin-mutant cancers. [PDF]
, 2019 Cohesin is a multiprotein ring that is responsible for cohesion of sister chromatids and formation of DNA loops to regulate gene expression. Genomic analyses have identified that the cohesin subunit STAG2 is frequently inactivated by mutations in cancer.
Ashworth, Alan +4 more
core +2 more sources