Results 31 to 40 of about 115,363 (220)

Visualization of targeted transduction by engineered lentiviral vectors [PDF]

Gene Therapy, 2008
We have reported a method to target lentiviral vectors to specific cell types. This method requires the incorporation of two distinct molecules on the viral vector surface: one is an antibody that renders the targeting specificity for the engineered vector, and the other is a fusogenic protein that allows the engineered vector to enter the target cell.
K-I, Joo, P, Wang
openaire   +2 more sources

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada, Barbero, Andrea, Candrian, Christian, Gianni-Barrera, Roberto, Jaquiery, Claude, Juelke, Henriette, Mainil-Varlet, Pierre, Martin, Ivan, Miot, Sylvie, Pelttari, Karoliina   +9 more
core   +1 more source

Antibody‐Based CAR T Cells Produced by Lentiviral Transduction [PDF]

Current Protocols in Immunology, 2020
AbstractOne promising approach to treat hematologic malignancies is the usage of patient‐derived CAR T cells. There are continuous efforts to improve the function of these cells, to optimize their receptor, and to use them for the treatment of additional types of cancer and especially solid tumors.
Prommersberger, Sabrina, Hudecek, Michael, Nerreter, Thomas   +2 more
openaire   +3 more sources

Directly injected lentiviral vector–based T cell vaccine protects mice against acute and chronic viral infection

JCI Insight, 2022
Lentiviral vector–based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of ...
Takuya Tada, Thomas D. Norton, Rebecca Leibowitz, Nathaniel R. Landau   +3 more
doaj   +1 more source

Evaluation of transduction efficiency in macrophage colony-stimulating factor differentiated human macrophages using HIV-1 based lentiviral vectors

BMC Biotechnology, 2011
Background Monocyte-derived macrophages contribute to atherosclerotic plaque formation. Therefore, manipulating macrophage function could have significant therapeutic value.
McCoy J Philip, Anzinger Joshua J, Leyva Francisco J, Kruth Howard S   +3 more
doaj   +1 more source

Avoiding lentiviral transduction culture induced MSC senescence [PDF]

Journal of Cellular and Molecular Medicine, 2009
Dear Editor: We read with interest the recent article by Bocker and colleagues in JCMM[1]. As noted by the authors, mesenchymal stem cells (MSC) enjoy considerable therapeutic potential in regenerative medicine, cell therapy for select genetic diseases and as immunomodulators [2, 3].
Pan, Yung-Wei, Kurre, Peter
openaire   +2 more sources

Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs

Stem Cell Research, 2015
Understanding how to achieve efficient transduction of hematopoietic stem and progenitor cells (HSPCs), while preserving their long-term ability to self-reproduce, is key for applying lentiviral-based gene engineering methods.
Duo Li, Erika Schlaepfer, Annette Audigé, Mary-Aude Rochat, Sandra Ivic, Caitlin N. Knowlton, Baek Kim, Oliver T. Keppler, Roberto F. Speck   +8 more
doaj   +1 more source

Signal transducer and activator of transcription 2 deficiency is a novel disorder of mitochondrial fission [PDF]

, 2015
Defects of mitochondrial dynamics are emerging causes of neurological disease. In two children presenting with severe neurological deterioration following viral infection we identified a novel homozygous STAT2 mutation, c.1836C4A (p.Cys612Ter), using ...
Anderson, G, Cale, CM, Chalasani, A, Chan, E, Duchen, MR, Gilmour, KC, Hambleton, S, Jacques, S, Osellame, LD, Plagnol, V, Qasim, W, Rahman, S, Shahni, R, Taanman, JW, Wadatilake, Y   +14 more
core   +1 more source

Efficiency of transgene expression in bovine cells varies according to cell type and gene transfer method

Revista Colombiana de Ciencias Pecuarias, 2019
Background: Production of transgenic animals is still a low-efficiency biotechnology, and simple alternatives should be used to improve the rate of transgenic bovine production by nuclear transfer.
Alinne G. Curcio, Fabiana F. Bressan, Carla S. Paes De Carvalho, Célia R. Quirino, Flavio V. Meirelles, Angelo J. B. Dias   +5 more
doaj   +1 more source

CREB is a critical regulator of normal hematopoiesis and leukemogenesis [PDF]

, 2008
The cAMP-responsive element binding protein (CREB) is a 43-kDa nuclear transcription factor that regulates cell growth, memory, and glucose homeostasis. We showed previously that CREB is amplified in myeloid leukemia blasts and expressed at higher levels
Akashi, Akashi, Bai, Bottazzi, Bruserud, Cheng, Christensen, Conkright, Crans-Vargas, Deepa B. Shankar, Dejah R. Judelson, Desdouets, Deshpande, Elliot M. Landaw, Fried, Fu, Hestdal, Impey, Ingrid Schmid, Jamieson, Jenny Chang, Jerry C. Cheng, Kathleen M. Sakamoto, Kentaro Kinjo, Kim, Kinjo, Kohn, Kondo, Koschmieder, Koya, Krishan, Kwon, Lee, Mayr, Mendoza, Miyoshi, Montminy, Ninomiya, Noriyuki Kasahara, Passegué, Ravi Bhatia, Rehemtulla, Renata Stripecke, Scherr, Shah, Shankar, Shankar, Shaywitz, Stripecke, Traver, Tuschl, Van Etten, Ventura, Winston S. Wu, Wong, Yuan   +55 more
core   +3 more sources