Results 31 to 40 of about 116,783 (314)

An efficient method for the transduction of primary pediatric glioma neurospheres

MethodsX, 2018
Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel, Dennis S. Metselaar, Piotr Waranecki, Gertjan J.L. Kaspers, Esther Hulleman   +4 more
doaj   +1 more source

Modular Lentiviral Vectors for Highly Efficient Transgene Expression in Resting Immune Cells

Viruses, 2021
Gene/cell therapies are promising strategies for the many presently incurable diseases. A key step in this process is the efficient delivery of genes and gene-editing enzymes to many cell types that may be resistant to lentiviral vector transduction ...
Christina Fichter, Anupriya Aggarwal, Andrew Kam Ho Wong, Samantha McAllery, Vennila Mathivanan, Bailey Hao, Hugh MacRae, Melissa J. Churchill, Paul R. Gorry, Michael Roche, Lachlan R. Gray, Stuart Turville   +11 more
doaj   +1 more source

Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Molecular Therapy: Methods & Clinical Development, 2016
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy, Chintan D Vin, Megan M Slough, Wayne R Gombotz, Brenna Kelley-Clarke   +4 more
doaj   +1 more source

Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors

Journal of Biological Engineering, 2009
Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
doaj   +1 more source

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. [PDF]

, 2015
Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene.
Baldwin, Kismet M, Campo-Fernandez, Beatriz, Cooper, Aaron R, Hoban, Megan D, Hollis, Roger P, Kaufman, Michael L, Kohn, Donald B, Lumaquin, Dianne, Romero, Zulema, Senadheera, Shantha, Urbinati, Fabrizia, Wang, Xiaoyan, Wherley, Jennifer   +12 more
core   +8 more sources

Expression of a large coding sequence: Gene therapy vectors for Ataxia Telangiectasia

Scientific Reports, 2023
Ataxia telangiectasia is a monogenetic disorder caused by mutations in the ATM gene. Its encoded protein kinase ATM plays a fundamental role in DNA repair of double strand breaks (DSBs).
Tanja Hirch, Nadine Brander, Franziska Schenk, Simon J. Pöllmann, Janine Reichenbach, Ralf Schubert, Ute Modlich   +6 more
doaj   +1 more source

Immunogenicity of targeted lentivectors [PDF]

, 2014
To increase the safety and possibly efficacy of HIV-1 derived lentivectors (LVs) as an anti-cancer vaccine, we recently developed the Nanobody (Nb) display technology to target LVs to antigen presenting cells (APCs).
Adotévi, Bennett, Bevan, Bhardwaj, Bhardwaj, Bhardwaj, Blander, Bonehill, Bonehill, Breckpot, Breckpot, Breckpot, Charleston, Chuah, Collins, Collins, Collins, Collins, Cosset, Diebold, Donckier, Eggermont, Enk, Figdor, Gajewski, Garcia, Groettrup, Inaba, Kast, Lahoutte, Lévy, McCullough, Medzhitov, Melief, Mellman, Mortha, Moser, Moser, Munn, Naldini, Neyns, Noguiez-Hellin, Nussenzweig, Palena, Raes, Reis e Sousa, Reiser, Reiser, Richardson, Romani, Roncarolo, Sakuma, Schroyens, Semesyuk, Song, Storm, Thielemans, Thielemans, Thielemans, Thielemans, Torretta, Turk, Van Kooyk, Wang, Wang, Yamaguchi, Yewdell, Yu   +67 more
core   +3 more sources

Directly injected lentiviral vector–based T cell vaccine protects mice against acute and chronic viral infection

JCI Insight, 2022
Lentiviral vector–based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of ...
Takuya Tada, Thomas D. Norton, Rebecca Leibowitz, Nathaniel R. Landau   +3 more
doaj   +1 more source

Perinatal Gene Transfer to the Liver [PDF]

, 2011
The liver acts as a host to many functions hence raising the possibility that any one may be compromised by a single gene defect. Inherited or de novo mutations in these genes may result in relatively mild diseases or be so devastating that death within
Buckley, SM, Chan, JK, Howe, SJ, McKay, TR, Rahim, AA, Waddington, SN, Ward, NJ   +6 more
core   +1 more source

Visualization of targeted transduction by engineered lentiviral vectors [PDF]

Gene Therapy, 2008
We have reported a method to target lentiviral vectors to specific cell types. This method requires the incorporation of two distinct molecules on the viral vector surface: one is an antibody that renders the targeting specificity for the engineered vector, and the other is a fusogenic protein that allows the engineered vector to enter the target cell.
K-I, Joo, P, Wang
openaire   +2 more sources