Results 51 to 60 of about 115,363 (220)

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. [PDF]

, 2015
Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene.
Baldwin, Kismet M, Campo-Fernandez, Beatriz, Cooper, Aaron R, Hoban, Megan D, Hollis, Roger P, Kaufman, Michael L, Kohn, Donald B, Lumaquin, Dianne, Romero, Zulema, Senadheera, Shantha, Urbinati, Fabrizia, Wang, Xiaoyan, Wherley, Jennifer   +12 more
core   +4 more sources

Efficient Transduction of T-Lymphocytes by Lentiviral Particles in Oncoimmunological Studies

Клиническая онкогематология, 2020
Aim. To compare different methods of lentivirus concentration in order to select the best way of providing high-level transduction for generating laboratory CAR-T cells. Materials & Methods. Concentration of lentiviral supernatant was carried out by 4
Ekaterina Konstantinovna Zaikova, K. A. Levchuk, D. Yu. Pozdnyakov, A. A. Daks, A. Yu. Zaritskey, A. V. Petukhov   +5 more
doaj   +1 more source

The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses [PDF]

, 2013
In recent years, high pathogenicity avian influenza (HPAI) virus, H5N1, low pathogenicity avian influenza (LPAI) virus, H9N2, and both HPAI and LPAI H7 viruses have proved devastating for the affected economies reliant on poultry industry, and have posed
Böttcher-Frieberthäuser, Eva, Cattoli, Giovanni, Ferrara, Francesca, Molesti, Eleonora, Temperton, Nigel, Terregino, Calogero   +5 more
core   +3 more sources

CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. [PDF]

, 2018
Hexanucleotide-repeat expansions in the C9ORF72 gene are the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD).
A Berson, A Jovičić, AA Dominguez, Aaron D. Gitler, AC Elden, AD Gitler, AD Gitler, AD Gitler, AE Renton, AM Jablonski, Amy Li, Ana Jovičić, AS Ng, BD Freibaum, C Sidrauski, C Sidrauski, C. Kimberly Tsui, D Ron, David W. Morgens, DW Morgens, DW Morgens, E Yeger-Lotem, EM Lynes, EY Son, FB Gao, Gregor Bieri, H Koike-Yusa, HJ Kim, HJ Kim, I Kwon, IR Mackenzie, IR Mackenzie, J Couthouis, J Joung, James Ousey, JC Christianson, JG Wideman, JP Taylor, Julien Couthouis, Justin K. Ichida, K Kanekura, K Mori, K Zhang, KH Lee, KM Green, KY Shi, L Ellgaard, L Huang, LA Becker, LA Gilbert, LA Gilbert, M Armakola, M DeJesus-Hernandez, M Neumann, M Prudencio, MA Horlbeck, Marc Tessier-Lavigne, MC Jonikas, MH Schludi, MI Love, Michael C. Bassik, Michael S. Haney, NF Liachko, Nicholas J. Kramer, Nicholas T. Hertz, O Shalem, OM Peters, PE Ash, Q Zhou, R Dafinca, R Lopez-Gonzalez, R Seijffers, RJ Platt, RM Deans, Rosanna Ma, S Boeynaems, S Boeynaems, S Ju, S Konermann, S Mizielinska, S Treusch, SC Ling, SJ Zhang, T Wang, T Westergard, T Zu, TF Gendron, W Cheng, X Wen, Y Lin, Y Zhou, Yingxiao Shi, YJ Zhang, YS Davidson, Z Shevtsova, Z Sun   +95 more
core   +1 more source

Kinetics of lentiviral vector transduction in human CD34+ cells [PDF]

Experimental Hematology, 2016
Unlike cell lines, human hematopoietic stem cells (HSCs) are less efficiently transduced with HIV-1 vectors, potentially limiting this approach. To investigate which step (internalization, reverse transcription, nuclear transport, and integration) limits lentiviral transduction, we evaluated the kinetics of lentiviral transduction in human CD34 ...
Naoya, Uchida, Rashidah, Green, Josiah, Ballantine, Luke P, Skala, Matthew M, Hsieh, John F, Tisdale   +5 more
openaire   +2 more sources

Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing

Viruses
Transduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the ...
Soledad Banos-Mateos, Carlos Lopez-Robles, María Eugenia Yubero, Aroa Jurado, Ane Arbelaiz-Sarasola, Andrés Lamsfus-Calle, Ane Arrasate, Carmen Albo, Juan Carlos Ramírez, Marie J. Fertin   +9 more
doaj   +1 more source

Efficient Transduction and Expansion of Ovine Macrophages for Gene Therapy Implementations

Veterinary Sciences, 2018
A number of bacteria provoking zoonotic diseases present intracellular survival and a host cell tropism limited to the monocyte/macrophage lineage. Thus, infection is rendered difficult to eradicate, causing chronic inflammatory reactions to the host and
Garyfalia Karponi, Spyridon Kritas, Evanthia Petridou, Eleni Papanikolaou   +3 more
doaj   +1 more source

Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging

Scientific Reports, 2022
Gene vectors to treat cystic fibrosis lung disease should be targeted to the conducting airways, as peripheral lung transduction does not offer therapeutic benefit. Viral transduction efficiency is directly related to the vector residence time.
Martin Donnelley, Patricia Cmielewski, Kaye Morgan, Juliette Delhove, Nicole Reyne, Alexandra McCarron, Nathan Rout-Pitt, Victoria Drysdale, Chantelle Carpentieri, Kathryn Spiers, Akihisa Takeuchi, Kentaro Uesugi, Naoto Yagi, David Parsons   +13 more
doaj   +1 more source

Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells

Molecular Therapy: Methods & Clinical Development, 2018
Lentiviral vector (LVV)-mediated transduction of human CD34+ hematopoietic stem and progenitor cells (HSPCs) holds tremendous promise for the treatment of monogenic hematological diseases.
Gretchen Lewis, Lauryn Christiansen, Jessica McKenzie, Min Luo, Eli Pasackow, Yegor Smurnyy, Sean Harrington, Philip Gregory, Gabor Veres, Olivier Negre, Melissa Bonner   +10 more
doaj   +1 more source

The determination of importance of sequences neighboring the Psi sequence in lentiviral vector transduction and packaging efficiency. [PDF]

PLoS ONE, 2012
A number of lentiviral vector systems have been developed for gene delivery and therapy by eliminating and/or modifying viral genetic elements. However, all lentiviral vector systems derived from HIV-1 must have a viral packaging signal sequence, Psi (Ψ),
Seon Hee Kim, Hyun Jeong Jun, Soo In Jang, Ji Chang You   +3 more
doaj   +1 more source