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Light sheet fluorescence microscopy of cleared human eyes [PDF]

Communications Biology, 2023
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Paques   +6 more
doaj   +4 more sources

Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain [PDF]

Methods and Protocols, 2023
Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing.
Youngjae Ryu, Yoonju Kim, Sang-Joon Park, Sung Rae Kim, Hyung-Jun Kim, Chang Man Ha   +5 more
doaj   +2 more sources

Meta-lens light-sheet fluorescence microscopy for in vivo imaging [PDF]

Nanophotonics, 2022
Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan, Tseng Ming Lun, Vyas Sunil, Hsieh Ting-Yu, Wu Jui-Ching, Chen Shang-Yang, Peng Hsiao-Fang, Su Vin-Cent, Huang Tzu-Ting, Kuo Hsin Yu, Chu Cheng Hung, Chen Mu Ku, Chen Jia-Wern, Chen Yu-Chun, Huang Kuang-Yuh, Kuan Chieh-Hsiung, Shi Xu, Misawa Hiroaki, Tsai Din Ping   +18 more
doaj   +2 more sources

In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development [PDF]

Scientific Data, 2022
Design Type(s) non-invasive long-term fluorescence live imaging Measurement Type(s) three-dimensional fluorophore distribution over time in a developing organism Technology Type(s) digital scanned laser light sheet fluorescence microscopy (DSLM) Factor ...
Frederic Strobl, Marc F. Schetelig, Ernst H. K. Stelzer   +2 more
doaj   +2 more sources

A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults [PDF]

Frontiers in Cell and Developmental Biology, 2022
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity.
Jayson J. Smith, Jayson J. Smith, Jayson J. Smith, Isabel W. Kenny, Isabel W. Kenny, Carsten Wolff, Carsten Wolff, Rachel Cray, Abhishek Kumar, Abhishek Kumar, David R. Sherwood, David R. Sherwood, David Q. Matus, David Q. Matus   +13 more
doaj   +2 more sources

Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy [PDF]

Biochemistry and Biophysics Reports
Biofilm formation exacerbates bacterial infections and interferes with industrial processes. However, the dynamics of biofilm development, especially if formed by a combination of more than one species, is not entirely understood.
Lenka Šmerdová, Tibor Füzik, Lucie Valentová, Pavol Bárdy, Michaela Procházková, Martina Pařenicová, Pavel Plevka   +6 more
doaj   +2 more sources

Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis [PDF]

Scientific Data
The red flour beetle (Tribolium castaneum) is a key model organism in developmental biology, genetics, and agricultural research. To address the limited availability of high-quality microscopy data documenting its embryonic morphogenesis, we assembled ...
Frederic Strobl, Pinelopi Goumenaki, Kristina Mirkes, Henrik Tonner, Mariia Golden, Julia Ratke, Franziska Krämer, Stefan Münster, Ernst H. K. Stelzer   +8 more
doaj   +2 more sources

Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging [PDF]

Frontiers in Neuroanatomy, 2021
Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns.
Zhouzhou Zhang, Zhouzhou Zhang, Xiao Yao, Xiao Yao, Xinxin Yin, Xinxin Yin, Zhangcan Ding, Tianyi Huang, Yan Huo, Runan Ji, Hanchuan Peng, Hanchuan Peng, Zengcai V. Guo, Zengcai V. Guo   +13 more
doaj   +2 more sources

Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning [PDF]

Intelligent Computing
Light-sheet fluorescence microscopy (LSFM) provides the benefit of optical sectioning coupled with rapid acquisition times, enabling high-resolution 3-dimensional imaging of large tissue-cleared samples.
Chen Li, Mani Ratnam Rai, Yuheng Cai, H. Troy Ghashghaei, Alon Greenbaum   +4 more
doaj   +2 more sources

Improved image contrast in nonlinear light-sheet fluorescence microscopy using i $$^2$$ 2 PIE Pulse compression [PDF]

Scientific Reports
Nonlinear microscopy has become an invaluable tool for biological imaging, offering high-resolution visualization of biological specimens. In this manuscript, we present the application of a spectral phase measurement technique, i $$^2$$ 2 PIE, to ...
Imraan Badrodien, Pieter H. Neethling, Gurthwin W. Bosman   +2 more
doaj   +2 more sources