Results 21 to 30 of about 104,318 (277)

Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]

Progress in Biophysics and Molecular Biology, 2022
Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez, Claire Jordan Brooks, Juan José Vaquero, Arrate Muñoz-Barrutia   +3 more
openaire   +3 more sources

Light Sheet Fluorescence Microscopy (LSFM) [PDF]

Current Protocols in Cytometry, 2015
Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams, Andrew F, Loftus, Sarah E, Dunn, Matthew S, Joens, James A J, Fitzpatrick   +4 more
openaire   +2 more sources

Metalens-Based Dual Light-Sheet Fluorescence Microscopy. [PDF]

Small Methods
Abstract Light‐sheet fluorescence microscopy (LSFM) provides optically sectioned fluorescence images with excellent background rejection for rapid and volumetric imaging. However, traditional LSFM typically relies on single‐sided illumination and the stripe artifacts due to partial obstruction or scattering of the illumination beam ...
Luo Y, Chang CC, Hsu HC, Huang BW, Vyas S, Tseng SJ, Chu CH, Tanaka T, Huang KY, Tsai DP.   +9 more
europepmc   +3 more sources

Three-photon light-sheet fluorescence microscopy [PDF]

Optics Letters, 2018
We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán, Federico M. Gasparoli, Jonathan Nylk, Pengfei Liu, Zhengyi Yang, Kishan Dholakia   +5 more
openaire   +5 more sources

Planar Airy beam light-sheet for two-photon microscopy [PDF]

, 2020
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro, Chennell, George, Chesters, Robert, Cho, Kwangwook, Forster, Robert, Hosny, Neveen A., Mitchell, Scott J., Mitchell, Thomas J., Seyforth, James A., Spickermann, Gunnar, Srivastava, Deepak P., Vernon, Anthony C., Vettenburg, Tom   +12 more
core   +4 more sources

Light-sheets and smart microscopy, an exciting future is dawning

Communications Biology, 2023
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
doaj   +1 more source

3D Volume Rendering of Invertebrates Using Light-Sheet Fluorescence Microscopy

Photonics, 2022
Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates.
Emilio J. Gualda
doaj   +1 more source

Light sheet fluorescence microscopy for neuroscience [PDF]

Journal of Neuroscience Methods, 2019
The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella, Gunn-Moore, Frank, Dholakia, Kishan   +2 more
openaire   +4 more sources

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]

, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K., Gunn-Moore, F. J., Mitchell, C., Nylk, J., Vettenburg, T.   +4 more
core   +2 more sources

Light Sheet Fluorescence Microscopy [PDF]

Journal of Histochemistry & Cytochemistry, 2011
Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
openaire   +2 more sources