Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy [PDF]
, 2017 Light sheet fluorescence microscopy enables high-resolution imaging of thick biological samples. By restricting the fluorescence excitation to a single plane, rapid wide-field image acquisition is possible with minimal sample exposure.
Corral, Angélica +4 more
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Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes. [PDF]
, 2016 The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D 'object plane'.
Bakowski, Malina A +6 more
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Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces
Frontiers in Cell and Developmental Biology, 2021 The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson +3 more
doaj +1 more source
Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells [PDF]
, 2013 Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence microscopy method allowing ...
Basu, Srinjan +7 more
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Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy [PDF]
, 2020 In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes.
Bainar, Petr +8 more
core +1 more source
Frontiers in Plant Science, 2020 Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá +7 more
doaj +1 more source
Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike +5 more
doaj +1 more source
Axial plane optical microscopy. [PDF]
, 2014 We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning.
Kim, Jeongmin +6 more
core +2 more sources
Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Methods and Protocols, 2019 Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista +4 more
doaj +1 more source
Light Sheet Fluorescence Microscopy
Proceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020 In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
openaire +2 more sources