Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]
, 2016 Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison +12 more
core +2 more sources
Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of The Royal Society Interface, 2014 We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Gao, Liang +3 more
openaire +3 more sources
Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]
Progress in Biophysics and Molecular Biology, 2022 In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P. +7 more
openaire +3 more sources
Bessel beam illumination reduces random and systematic errors in quantitative functional studies using light-sheet microscopy [PDF]
, 2018 Light-sheet microscopy (LSM), in combination with intrinsically transparent zebrafish larvae, is a choice method to observe brain function with high frame rates at cellular resolution.
Alterini, Tommaso +8 more
core +15 more sources
Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]
EPJ Web of Conferences, 2023 Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia +5 more
doaj +1 more source
Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy
Cell Reports, 2012 Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok +5 more
doaj +3 more sources
Whole-brain vasculature reconstruction at the single capillary level [PDF]
, 2018 The distinct organization of the brain’s vascular network ensures that it is adequately supplied with oxygen and nutrients. However, despite this fundamental role, a detailed reconstruction of the brain-wide vasculature at the capillary level remains ...
Allegra Mascaro, Anna Letizia +8 more
core +2 more sources
Multi-photon attenuation-compensated light-sheet fluorescence microscopy [PDF]
Scientific Reports, 2019 Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an ...
Veettikazhy, Madhu +7 more
openaire +5 more sources
Frontiers in Neuroanatomy, 2021 The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli +19 more
doaj +1 more source
Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates [PDF]
, 2016 Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-
Carling, D +7 more
core +1 more source