Results 21 to 30 of about 218,193 (270)
Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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eLife, 2020 Cancer clone evolution takes place within tissue ecosystem habitats. But, how exactly tumors arise from a few malignant cells within an intact epithelium is a central, yet unanswered question.
Ashna Alladin +8 more
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Confocal multiview light-sheet microscopy [PDF]
Nature Communications, 2015 AbstractSelective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image ...
de Medeiros, Gustavo +9 more
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Planar Airy beam light-sheet for two-photon microscopy [PDF]
, 2020 We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-
Almada, Pedro +12 more
core +4 more sources
Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Nanophotonics, 2022 Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging.
Luo Yuan +18 more
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Light sheet fluorescence microscopy of cleared human eyes
Communications Biology, 2023 We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling.
Marie Darche +6 more
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Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light [PDF]
, 2014 The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Dholakia, K. +4 more
core +2 more sources
Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus
Frontiers in Cell and Developmental Biology, 2021 Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution.
Simona Machacova +4 more
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Light-sheet microscopy: a tutorial [PDF]
, 2018 This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the
Andilla, Jordi +3 more
core +3 more sources
A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues
Cell Reports, 2020 Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving
Yanlu Chen +17 more
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