Light sheet microscopy with acoustic sample confinement [PDF]
Nature Communications, 2019 Here, the authors integrate an acoustic trap with a compact light sheet fluorescence microscope to provide contactless sample confinement of biological organisms.
Zhengyi Yang +9 more
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High-resolution open-top axially swept light sheet microscopy [PDF]
BMC Biology, 2023 Background Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain.
Soohyun Park +3 more
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Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy [PDF]
eLife, 2020 In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes.
Sona Valuchova +8 more
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Projective light-sheet microscopy with flexible parameter selection [PDF]
Nature CommunicationsProjection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up.
Bingying Chen +11 more
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Self-Supervised and Zero-Shot Learning in Multi-Modal Raman Light Sheet Microscopy [PDF]
SensorsAdvancements in Raman light sheet microscopy have provided a powerful, non-invasive, marker-free method for imaging complex 3D biological structures, such as cell cultures and spheroids.
Pooja Kumari +2 more
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In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy [PDF]
Nature CommunicationsSingle molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution.
Marine Cabillic +14 more
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Open-top Bessel beam two-photon light sheet microscopy for three-dimensional pathology [PDF]
eLifeNondestructive pathology based on three-dimensional (3D) optical microscopy holds promise as a complement to traditional destructive hematoxylin and eosin (H&E) stained slide-based pathology by providing cellular information in high throughput manner ...
Won Yeong Park +6 more
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Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy [PDF]
Scientific ReportsLight sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents.
Alice M. Jacob +13 more
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Extended field-of-view light-sheet microscopy [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a ...
Vettenburg Tom
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Light Sheet Fluorescence Microscopy Using Incoherent Light Detection
Engineering Proceedings, 2023 We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy.
Mariana Potcoava +3 more
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