Results 11 to 20 of about 218,213 (290)
Technical implementations of light sheet microscopy [PDF]
Microscopy Research and Technique, 2018 Fluorescence-based microscopy is among the most successful methods in biological studies. It played a critical role in the visualization of subcellular structures and in the analysis of complex cellular processes, and it is nowadays commonly employed in ...
Braeckmans, Kevin +5 more
core +5 more sources
Applications of Light-Sheet Microscopy in Microdevices [PDF]
Frontiers in Neuroanatomy, 2019 Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories for quite some time, mainly as custom-made systems, with imaging applications ranging from single cells (in the micrometer scale) to small organisms (in the ...
Ignacio Albert-Smet +11 more
doaj +6 more sources
Multicolor two-photon light-sheet microscopy [PDF]
Nature Methods, 2014 International audienceTwo-photon microscopy is the most effective approach for deep-tissue fluorescence cellular imaging; however, its application to high-throughput or high-content imaging is often hampered by low pixel rates, challenging multicolor ...
Emmanuel Beaurepaire +8 more
core +8 more sources
Acoustic light-sheet microscopy [PDF]
, 2021 AbstractLight-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet microscopy (acoustic LSM), which actively manipulates the light propagation inside a large sample to ...
S. Wunderl +12 more
openaire +2 more sources
Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj +1 more source
Computational hyperspectral light-sheet microscopy
Optics Express, 2022 We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens.
Crombez, Sébastien +3 more
openaire +4 more sources
Hyperspectral light sheet microscopy [PDF]
Nature Communications, 2015 AbstractTo study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three ...
Wiebke Jahr +4 more
openaire +4 more sources
eduSPIM: Light Sheet Microscopy in the Museum. [PDF]
PLoS ONE, 2016 LIGHT SHEET MICROSCOPY IN THE MUSEUM:Light sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences.
Wiebke Jahr +3 more
doaj +1 more source
Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
doaj +1 more source
Light-Sheet Microscopy in Neuroscience [PDF]
Annual Review of Neuroscience, 2019 Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse range of neuroscience applications. Unlike point-scanning techniques such as confocal and two-photon microscopy, light-sheet microscopes illuminate an entire plane of tissue, while imaging this plane onto a camera.
Elizabeth M C, Hillman +3 more
openaire +2 more sources