Results 1 to 10 of about 98,017 (217)

Preparation and Negative Staining for Visualization of Cyanoglobule Lipid Droplets Using Transmission Electron Microscopy. [PDF]

Bio Protoc
Lipid droplets have emerged as dynamic organelles involved in diverse cellular processes beyond simple lipid storage. In plants and cyanobacteria, growing evidence highlights their importance in stress adaptation and signaling, yet methods to study their
Susanto FA, Withrow A, Lundquist PK.
europepmc   +2 more sources

Volumetric analysis of lymphocyte lipid droplets in type 2 diabetes mellitus patients with hyperlipidemia [PDF]

Medicinski Podmladak, 2023
Introduction: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by inadequate glucose homeostasis. A common occurrence of T2DM is diabetic dyslipidemia.
Živković Aleksa, Ćirić Darko, Martinović Tamara, Jovanović Sofija, Kravić-Stevović Tamara   +4 more
doaj   +1 more source

Small GTPase Rab40c associates with lipid droplets and modulates the biogenesis of lipid droplets. [PDF]

PLoS ONE, 2013
The subcellular location and cell biological function of small GTPase Rab40c in mammalian cells have not been investigated in detail. In this study, we demonstrated that the exogenously expressed GFP-Rab40c associates with lipid droplets marked by ...
Ran Tan, Weijie Wang, Shicong Wang, Zhen Wang, Lixiang Sun, Wei He, Rong Fan, Yunhe Zhou, Xiaohui Xu, Wanjin Hong, Tuanlao Wang   +10 more
doaj   +1 more source

Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins

STAR Protocols, 2022
Summary: Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins.
Zelun Zhi, Xuejing Ma, Chang Zhou, Adam Mechler, Shuyan Zhang, Pingsheng Liu   +5 more
doaj   +1 more source

Nuclear lipid droplets and nuclear damage in Caenorhabditis elegans.

PLoS Genetics, 2021
Fat stored in the form of lipid droplets has long been considered a defining characteristic of cytoplasm. However, recent studies have shown that nuclear lipid droplets occur in multiple cells and tissues, including in human patients with fatty liver ...
Jose Verdezoto Mosquera, Meghan C Bacher, James R Priess   +2 more
doaj   +1 more source

The Janus-Faced Role of Lipid Droplets in Aging: Insights from the Cellular Perspective

Biomolecules, 2023
It is widely accepted that nine hallmarks—including mitochondrial dysfunction, epigenetic alterations, and loss of proteostasis—exist that describe the cellular aging process.
Nikolaus Bresgen, Melanie Kovacs, Angelika Lahnsteiner, Thomas Klaus Felder, Mark Rinnerthaler   +4 more
doaj   +1 more source

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

eLife, 2021
Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in ...
Meredith H Wilson, Stephen C Ekker, Steven A Farber   +2 more
doaj   +1 more source

Redistribution of macrophage cholesteryl ester hydrolase from cytoplasm to lipid droplets upon lipid loading1

Journal of Lipid Research, 2005
Hydrolysis of intracellular cholesteryl esters (CEs) represents the first step in the removal of cholesterol from lipid-laden foam cells associated with atherosclerotic lesions.
Bin Zhao, Bernard J. Fisher, Richard W. St. Clair, Lawrence L. Rudel, Shobha Ghosh   +4 more
doaj   +1 more source

Effect of substrates on the cyclic AMP-dependent lipolytic reaction of hormone-sensitive lipase.

Journal of Lipid Research, 1994
Cyclic AMP-dependent activation of hormone-sensitive lipase (HSL) stimulated lipolysis of [3H]triolein emulsified with gum arabic, but not that of endogenous lipid droplets from rat fat cells.
H Okuda, C Morimoto, T Tsujita
doaj   +1 more source

Lipid droplets modulate proteostasis, SQST-1/SQSTM1 dynamics, and lifespan in C. elegans

iScience, 2023
Summary: In several long-lived Caenorhabditis elegans strains, such as insulin/IGF-1 receptor daf-2 mutants, enhanced proteostatic mechanisms are accompanied by elevated intestinal lipid stores, but their role in longevity is unclear.
Anita V. Kumar, Joslyn Mills, Wesley M. Parker, Joshua A. Leitão, Diego I. Rodriguez, Sandrine E. Daigle, Celeste Ng, Rishi Patel, Joseph L. Aguilera, Joseph R. Johnson, Shi Quan Wong, Louis R. Lapierre   +11 more
doaj   +1 more source