Pt-LIS1 participates nuclear deformation and acrosome formation via regulating Dynein-1 during spermatogenesis in Portunus trituberculatus [PDF]
Scientific ReportsSpermatogenesis involves complex dynamic mechanisms. Dynein-1 is a key carrier in cellular cargo transport, participating in nuclear deformation and acrosome formation during spermatogenesis.
Le Chang +6 more
doaj +3 more sources
Load-induced enhancement of Dynein force production by LIS1–NudE in vivo and in vitro [PDF]
Nature Communications, 2016 Transport of large cargo through the cytoplasm can encounter physical impediments which should be overcome. Here the authors show that lipid droplets constrained by an optical trap respond with an increase in dynein-mediated force that is dependent on ...
Dail Chapman, Richard B Vallee
exaly +3 more sources
Brain Pathways in LIS1-Associated Lissencephaly Revealed by Diffusion MRI Tractography [PDF]
Brain Sciences, 2023 Lissencephaly (LIS) is a rare neurodevelopmental disorder with severe symptoms caused by abnormal neuronal migration during cortical development. It is caused by both genetic and non-genetic factors.
Alpen Ortug +5 more
doaj +4 more sources
Nde1 promotes Lis1-mediated activation of dynein [PDF]
Nature Communications, 2023 Cytoplasmic dynein drives the motility and force generation functions towards the microtubule minus end. The assembly of dynein with dynactin and a cargo adaptor in an active transport complex is facilitated by Lis1 and Nde1/Ndel1.
Yuanchang Zhao, Sena Oten, Ahmet Yildiz
doaj +2 more sources
LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways [PDF]
Nature Communications, 2023 Lissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the molecular motor cytoplasmic dynein activity. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs ...
Aditya Kshirsagar +15 more
doaj +2 more sources
Cryo-EM captures early intermediate steps in dynein activation by LIS1 [PDF]
Nature CommunicationsCytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited Phi state and requires conformational changes to assemble with its cofactors and form active transport complexes.
Kendrick H. V. Nguyen +4 more
doaj +2 more sources
Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein [PDF]
Nature Communications, 2023 Processive transport by the microtubule motor cytoplasmic dynein requires the regulated assembly of a dynein-dynactin-adapter complex. Interactions between dynein and dynactin were initially ascribed to the dynein intermediate chain N-terminus and the ...
Kyoko Okada +6 more
doaj +2 more sources
Structures of human dynein in complex with the lissencephaly 1 protein, LIS1 [PDF]
eLife, 2023 The lissencephaly 1 protein, LIS1, is mutated in type-1 lissencephaly and is a key regulator of cytoplasmic dynein-1. At a molecular level, current models propose that LIS1 activates dynein by relieving its autoinhibited form.
Janice M Reimer +3 more
doaj +2 more sources
Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes. [PDF]
PLoS Biology, 2011 Radial glial cells (RGCs) are distinctive neural stem cells with an extraordinary slender bipolar morphology and dual functions as precursors and migration scaffolds for cortical neurons.
Ashley S Pawlisz, Yuanyi Feng
doaj +3 more sources
MiR-380 inhibits the proliferation and invasion of cholangiocarcinoma cells by silencing LIS1 [PDF]
Cancer Cell InternationalBackground The objective of this study was to determine the role and regulatory mechanism of miR-380 in cholangiocarcinoma. Methods The TargetScan database and a dual-luciferase reporter assay system were used to determine if LIS1 was a target gene of ...
Zhicheng Wei +7 more
doaj +2 more sources