Results 81 to 90 of about 23,262 (271)

Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging

Cell Reports Methods, 2023
Summary Confocal Raman spectral imaging (RSI) enables high-content, label-free visualization of a wide range of molecules in biological specimens without sample preparation. However, reliable quantification of the deconvoluted spectra is needed.
Vernon LaLone, A. Aizenshtadt, John A. Goertz, F. S. Skottvoll, Marco Barbero Mota, Jun-Lin You, Xiaoyu Zhao, Henriette Engen Berg, Justyna Stokowiec, Minzhi Yu, A. Schwendeman, H. Scholz, S. Wilson, Stefan Krauss, M. Stevens   +14 more
semanticscholar   +1 more source

Network divergence analysis identifies adaptive gene modules and two orthogonal vulnerability axes in pancreatic cancer

Molecular Oncology, EarlyView.
Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson, Lyanne Delgado‐Coka, Natalia Marchenko, Luisa F. Escobar‐Hoyos, Kenneth R. Shroyer, Alisa Yurovsky, Trey Ideker, Gabor Balázsi, Thomas MacCarthy, Scott Powers   +9 more
wiley   +1 more source

Region‐to‐Region Unidirectional Connection In Vitro Brain Model for Studying Directional Propagation of Neuropathologies

Advanced Functional Materials, EarlyView.
A unidirectional cerebral organoid–organoid neural circuit is established using a microfluidic platform, enabling controlled directional propagation of electrical signals, neuroinflammatory cues, and neurodegenerative disease–related proteins between spatially separated organoids.
Kyeong Seob Hwang, Hui‐Wen Liu, Hyogeun Shin, Hyun Wook Kang, Minjin Kang, Jongbaeg Kim, Il‐Joo Cho, Nakwon Choi, Seok Chung, Hong Nam Kim   +9 more
wiley   +1 more source

Nanodiamond‐Based Quantum Sensing Reveals Changes in Endo‐Lysosomal Activity of Stimulated Cardiac Fibroblasts in a Cellular Fibrosis Model

Advanced Functional Materials, EarlyView.
Quantum sensing reveals intricate patterns linking endo‐lysosomal maturation to cardiac fibrosis progression, highlighting complexity in cellular remodeling. This study investigates fibroblast‐to‐myofibroblast transition under cell aging, stiffness, and TGF‐β stimulation, comparing nanodiamond uptake, endo‐lysosomal dynamics, and free radical ...
Aldona Mzyk, Ezgi Yilmaz, Giovanni D Angelo, Kirstine Berg‐Sørensen   +3 more
wiley   +1 more source

Human induced pluripotent stem cells‐derived liver organoids grown on a Biomimesys® hyaluronic acid‐based hydroscaffold as a new model for studying human lipoprotein metabolism

Bioengineering & Translational Medicine
The liver plays a key role in the metabolism of lipoproteins, controlling both production and catabolism. To accelerate the development of new lipid‐lowering therapies in humans, it is essential to have a relevant in vitro study model available.
M. Roudaut, A. Caillaud, Zied Souguir, Lise Bray, A. Girardeau, Antoine Rimbert, Mikaël Croyal, Gilles Lambert, Murielle Patitucci, Gaspard Delpouve, Élodie Vandenhaute, C. Le May, Nathalie Maubon, Bertrand Cariou, K. Si-Tayeb   +14 more
semanticscholar   +1 more source

Evaluation of the efficacy of mitochondrial fission inhibitor (Mdivi-1) using non-alcoholic steatohepatitis (NASH) liver organoids

Frontiers in Pharmacology, 2023
Non-alcoholic steatohepatitis (NASH) is known to progress to cirrhosis and hepatocellular carcinoma in some patients. Although NASH is associated with abnormal mitochondrial function related to lipid metabolism, mechanisms for the development and ...
M. Elbadawy, K. Tanabe, Haru Yamamoto, Y. Ishihara, Maria Mochizuki, Amira Abugomaa, H. Yamawaki, M. Kaneda, T. Usui, Kazuaki Sasaki   +9 more
semanticscholar   +1 more source

Translational Considerations for Injectable Biomaterials and Bioscaffolds to Repair and Regenerate Brain Tissue

Advanced Healthcare Materials, EarlyView.
The repair and regeneration of brain tissue faces both biological and technical challenges. Injectable bioscaffolds offer new opportunities to stimulate tissue regrowth in the brain by recruiting neural stem cells. Here, the translational issues are reviewed that need to be address to advance this promising new therapeutic approach from the bench to ...
Michel Modo, Alena Kisel
wiley   +1 more source

3D Co-Culture of Hepatocyte, a Hepatic Stellate Cell Line, and Stem Cells for Developing a Bioartificial Liver Prototype

International Journal of Technology, 2020
A liver organoid is an in vitro reconstruction of the liver that mimics the in vivo liver microstructure and performs liver functions. Liver organoids can be used for drug testing, as a model of liver disease pathogenesis, and as a bioartificial ...
Christine Verawaty Sibuea, Jeanne Pawitan, Radiana Antarianto, Cynthia O.M. Jasirwan, Imelda Rosalyn Sianipar, Evah Luviah, Retno Wahyu Nurhayati, Wildan Mubarok, Nuzli Fahdia Mazfufah   +8 more
doaj   +1 more source

Liver organoids: from basic research to therapeutic applications [PDF]

Gut, 2019
Organoid cultures have emerged as an alternative in vitro system to recapitulate tissues in a dish. While mouse models and cell lines have furthered our understanding of liver biology and associated diseases, they suffer in replicating key aspects of human liver tissue, in particular its complex architecture and metabolic functions.
Prior, Nicole, Inacio, Patricia, Huch, Meritxell   +2 more
openaire   +6 more sources

Research progress and application of liver organoids for disease modeling and regenerative therapy

Journal of molecular medicine
The liver is a major metabolic organ of the human body and has a high incidence of diseases. In recent years, the annual incidence of liver disease has increased, seriously endangering human life and health.
Yang Hu, Qiao Geng, Lu Wang, Yi Wang, Chuyue Huang, Zhimin Fan, Desong Kong   +6 more
semanticscholar   +1 more source