Results 101 to 110 of about 672,449 (294)

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Inhibition of CDK9 enhances AML cell death induced by combined venetoclax and azacitidine

Molecular Oncology, EarlyView.
The CDK9 inhibitor AZD4573 downregulates c‐MYC and MCL‐1 to induce death of cytarabine (AraC)‐resistant AML cells. This enhances VEN + AZA‐induced cell death significantly more than any combination of two of the three drugs in AraC‐resistant AML cells.
Shuangshuang Wu, Jianlei Zhao, Aaban Asfar Azmi, Avanti Gupte, Jenna Thibodeau, Shuang Liu, Jinli Yang, Guan Wang, Holly Edwards, Lisa A. Polin, Juiwanna Kushner, Sijana H. Dzinic, Kathryn White, Julie Boerner, Maik Hüttemann, Jay Yang, Yue Wang, Jeffrey W. Taub, Yubin Ge   +18 more
wiley   +1 more source

A synthetic benzoxazine dimer derivative targets c‐Myc to inhibit colorectal cancer progression

Molecular Oncology, EarlyView.
Benzoxazine dimer derivatives bind to the bHLH‐LZ region of c‐Myc, disrupting c‐Myc/MAX complexes, which are evaluated from SAR analysis. This increases ubiquitination and reduces cellular c‐Myc. Impairing DNA repair mechanisms is shown through proteomic analysis.
Nicharat Sriratanasak, Bodee Nutho, Worawat Wattanathana, Narumon Phaonakrop, Bunnatut Panasawatwong, Katharina Erlenbach‐Wuensch, Sittiruk Roytrakul, Regine Schneider‐Stock, Pithi Chanvorachote   +8 more
wiley   +1 more source

Optimizing sequencing protocols for leaderboard metagenomics by combining long and short reads. [PDF]

, 2019
As metagenomic studies move to increasing numbers of samples, communities like the human gut may benefit more from the assembly of abundant microbes in many samples, rather than the exhaustive assembly of fewer samples.
Arthur, Timothy D, Bankevich, Anton, Boland, Brigid S, Brennan, Caitriona, Chang, John T, Chen, Feng, Conrad, Douglas J, Dang, Jason W, Dorrestein, Pieter C, Fedarko, Marcus, Gaffney, James, Green, Cliff, Humphrey, Greg C, Jepsen, Kristen, Khosroheidari, Mahdieh, Knight, Rob, Liyanage, Marlon, Martino, Cameron, Minich, Jeremiah, Nurk, Sergey, Pevzner, Pavel A, Phelan, Vanessa V, Quinn, Robert A, Rana, Tariq M, Salido, Rodolfo A, Sandborn, William J, Sanders, Jon G, Sanders, Karenina, Smarr, Larry, Xu, Zhenjiang Z, Zhu, Qiyun   +30 more
core   +1 more source

PacBio long-read sequencing v1

The barcoded library is also sequenced via PacBio long-read sequencing to associate variants with particular barcodes. This allows for the generation of a variant-barcode map along the length of the protein of interest, to which variant scores can be mapped.
Nicholas A. Popp, Melinda K. Wheelock, Raining Wang, Rachel L. Powell, Douglas M. Fowler   +4 more
openaire   +1 more source

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

Genomic surveillance of multidrug-resistant organisms based on long-read sequencing

Genome Medicine
Background Multidrug-resistant organisms (MDRO) pose a significant threat to public health worldwide. The ability to identify antimicrobial resistance determinants, to assess changes in molecular types, and to detect transmission are essential for ...
Fabian Landman, Casper Jamin, Angela de Haan, Sandra Witteveen, Jeroen Bos, Han G. J. van der Heide, Leo M. Schouls, Antoni P. A. Hendrickx, Dutch CPE/MRSA surveillance study group   +8 more
doaj   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Long-read sequencing reveals the full diversity and structure of host sequences integrated into AcMNPV baculovirus genomes during infection [PDF]

, 2018
Horizontal transfers (HTs) of genetic material is increasingly recognized as a major force shaping genomic evolution in some eukaryotes, but the mechanisms underlying these HTs are still unknown. lt has been proposed that viruses cou Id act as vectors of
Cordaux, R., Gilbert, C., Herniou, E., Labrousse, C., Loiseau, V., Moreau, Yannis   +5 more
core  

Improving sequencing by tunneling with multiplexing and cross-correlations

, 2014
Sequencing by tunneling is a next-generation approach to read single-base information using electronic tunneling transverse to the single-stranded DNA (ssDNA) backbone while the latter is translocated through a narrow channel.
Balatsky, A. V., Boynton, P., Di Ventra, M., Schuller, I. K.   +3 more
core   +1 more source