Results 11 to 20 of about 383,643 (254)

Long-Read Nanopore-Based Sequencing of Anelloviruses

Viruses
Routinely used metagenomic next-generation sequencing (mNGS) techniques often fail to detect low-level viremia (
Raghavendran Anantharam, Dylan Duchen, Andrea L. Cox, Winston Timp, David L. Thomas, Steven J. Clipman, Abraham J. Kandathil   +6 more
doaj   +3 more sources

Applications of long-read sequencing to Mendelian genetics

Genome Medicine, 2023
Advances in clinical genetic testing, including the introduction of exome sequencing, have uncovered the molecular etiology for many rare and previously unsolved genetic disorders, yet more than half of individuals with a suspected genetic disorder ...
Francesco Kumara Mastrorosa, Danny E. Miller, Evan E. Eichler   +2 more
doaj   +3 more sources

QitanTech Nanopore Long-Read Sequencing Enables Rapid Resolution of Complete Genomes of Multi-Drug Resistant Pathogens

Frontiers in Microbiology, 2022
Advancement of novel sequencing technologies facilitates modern life science and medicine unprecedentedly. Exploring complete genome sequences of bacteria by long-read sequencing technology is significant for microbial genomics research.
Kai Peng, Kai Peng, Yi Yin, Yi Yin, Yan Li, Yan Li, Shangshang Qin, Yuan Liu, Yuan Liu, Xiaorong Yang, Zhiqiang Wang, Zhiqiang Wang, Ruichao Li, Ruichao Li   +13 more
doaj   +3 more sources

Application of long-read sequencing to the detection of structural variants in human cancer genomes

Computational and Structural Biotechnology Journal, 2021
In recent years, the so-called long-read sequencing technology has had a substantial impact on various aspects of genome sciences. Here, we introduce recent studies of cancerous structural variants (SVs) using long-read sequencing technologies, namely ...
Yoshitaka Sakamoto, Suzuko Zaha, Yutaka Suzuki, Masahide Seki, Ayako Suzuki   +4 more
doaj   +3 more sources

Genome sequencing using long-read sequencing

Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 2023
Juan Guillermo McEwen, Oscar Mauricio Gómez   +1 more
doaj   +2 more sources

Why Are Long-Read Sequencing Methods Revolutionizing Microbiome Analysis? [PDF]

Microorganisms
Most of the knowledge available on the composition and functionality of microbial communities in different ecosystems comes from short-read sequencing methods.
Adriana González, Asier Fullaondo, Adrian Odriozola   +2 more
doaj   +2 more sources

The application of long-read sequencing in clinical settings

Human Genomics, 2023
Long-read DNA sequencing technologies have been rapidly evolving in recent years, and their ability to assess large and complex regions of the genome makes them ideal for clinical applications in molecular diagnosis and therapy selection, thereby ...
Josephine B. Oehler, Helen Wright, Zornitza Stark, Andrew J. Mallett, Ulf Schmitz   +4 more
doaj   +6 more sources

Long-read sequencing transcriptome quantification with lr-kallisto. [PDF]

PLoS Computational Biology
RNA abundance quantification has become routine and affordable thanks to high-throughput "short-read" technologies that provide accurate molecule counts at the gene level.
Rebekah K Loving, Delaney K Sullivan, Fairlie Reese, Elisabeth Rebboah, Jasmine Sakr, Narges Rezaie, Heidi Y Liang, Ghassan Filimban, Shimako Kawauchi, A Sina Booeshaghi, Páll Melsted, Conrad Oakes, Diane Trout, Brian A Williams, Grant R MacGregor, Barbara J Wold, Ali Mortazavi, Lior Pachter   +17 more
doaj   +2 more sources

Long-read sequencing for diagnosis of genetic myopathies

BMJ Neurology Open
Genetic myopathies are caused by pathogenic variants in >300 genes across the nuclear and mitochondrial genomes. Although short-read next-generation sequencing (NGS) has revolutionised the diagnosis of genetic disorders, large and/or complex genetic ...
Gianina Ravenscroft, Roula Ghaoui, Ryan Davis, Kishore Raj Kumar, Karl Ng, Marina Kennerson, Dennis Yeow, Ira Deveson, Laura Ivete Rudaks, Pak Leng Cheong   +9 more
doaj   +3 more sources

Nanopore long-read sequencing of circRNAs [PDF]

Methods, 2021
Circular RNA (circRNA) is a group of highly stable RNA molecules with suggested roles in development and disease. They derive from linear pre-mRNAs when a 5'-splice site splices back to an upstream 3'-splice site in a process termed back-splicing. Most circRNAs are multi-exonic and may contain several thousand nucleotides.
Rahimi, Karim, Nielsen, Anne Færch, Venø, Morten, Kjems, Jorgen   +3 more
openaire   +3 more sources