Results 31 to 40 of about 7,947,893 (291)

Geoseq: a tool for dissecting deep-sequencing datasets [PDF]

open access: yes, 2010
Gurtowski J, Cancio A, Shah H, et al. Geoseq: a tool for dissecting deep-sequencing datasets. BMC Bioinformatics. 2010;11(1): 506.Background Datasets generated on deep-sequencing platforms have been deposited in various public repositories such as the ...
Gurtowski, James   +20 more
core   +1 more source

Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales

open access: yesApplications in Plant Sciences, 2023
Premise We present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output.
Gisel Y. De La Cerda   +11 more
doaj   +1 more source

Long-Read Sequencing Annotation of the Transcriptome in DNA-PK Inactivated Cells

open access: yesFrontiers in Oncology, 2022
The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) with a Ku70/Ku80 heterodimer constitutes the intact DNA-PK kinase, which is an upstream component of the DNA repair machinery that signals the DNA damage, orchestrates the DNA repair, and ...
Liwei Song   +10 more
doaj   +1 more source

QitanTech Nanopore Long-Read Sequencing Enables Rapid Resolution of Complete Genomes of Multi-Drug Resistant Pathogens

open access: yesFrontiers in Microbiology, 2022
Advancement of novel sequencing technologies facilitates modern life science and medicine unprecedentedly. Exploring complete genome sequences of bacteria by long-read sequencing technology is significant for microbial genomics research.
Kai Peng   +13 more
doaj   +1 more source

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]

open access: yes, 2014
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A   +83 more
core   +1 more source

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

open access: yes, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan   +23 more
core   +1 more source

Long-read sequencing data analysis for yeasts [PDF]

open access: yesNature Protocols, 2018
Long-read sequencing technologies have become increasingly popular due to their strengths in resolving complex genomic regions. As a leading model organism with small genome size and great biotechnological importance, the budding yeast Saccharomyces cerevisiae has many isolates currently being sequenced with long reads.
Yue, Jia-Xing, Liti, Gianni
openaire   +3 more sources

Utility of long-read sequencing for All of Us

open access: yesNature Communications, 2023
Abstract The All of Us (AoU) initiative aims to sequence the genomes of over one million Americans from diverse ethnic backgrounds to improve personalized medical care. In a recent technical pilot, we compare the performance of traditional short-read sequencing with long-read sequencing in a small cohort of samples from the HapMap ...
M. Mahmoud   +13 more
openaire   +4 more sources

Long-read sequence assembly: a technical evaluation in barley [PDF]

open access: yesThe Plant Cell, 2021
AbstractSequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources and often several complementary sequence assembly and genome mapping approaches. The recent development of fast and accurate long-read sequencing by circular consensus sequencing (CCS) on the PacBio platform may greatly ...
Mascher, Martin   +21 more
openaire   +6 more sources

Long-read sequencing provides uniform coverage across deletion-prone region in the virus genome.

open access: yes, 2022
Panel A shows the percentage of the virus genome mapped sequencing reads (A) and virus genome fraction covered with Nanopore sequencing data (B), in the positive control (VR1986D ATCC SARS-CoV-2 RNA).
Li Chen (5749)   +9 more
core   +1 more source

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