Results 31 to 40 of about 7,947,893 (291)
Geoseq: a tool for dissecting deep-sequencing datasets [PDF]
, 2010 Gurtowski J, Cancio A, Shah H, et al. Geoseq: a tool for dissecting deep-sequencing datasets. BMC Bioinformatics. 2010;11(1): 506.Background
Datasets generated on deep-sequencing platforms have been deposited in various public repositories such as the ...Gurtowski, James, Sachidanandam, Ravi, Robert Homann, Shah Hardik, Chaya Levovitz, Ravi Sachidanandam, Hardik Shah, James Gurtowski, Cancio, Anthony, Levovitz Chaya, George Ajish, Cancio Anthony, Homann, Robert, Anthony Cancio, Shah, Hardik, Homann Robert, Levovitz, Chaya, Sachidanandam Ravi, Ajish George, George, Ajish, Gurtowski James +20 morecore +1 more sourceBalancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales
Applications in Plant Sciences, 2023 Premise We present approaches used to generate long‐read Nanopore sequencing reads for the Liliales and demonstrate how modifications to standard protocols directly impact read length and total output.Gisel Y. De La Cerda, Jacob B. Landis, Evan Eifler, Adriana I. Hernandez, Fay‐Wei Li, Jing Zhang, Carrie M. Tribble, Nisa Karimi, Patricia Chan, Thomas Givnish, Susan R. Strickler, Chelsea D. Specht +11 moredoaj +1 more sourceLong-Read Sequencing Annotation of the Transcriptome in DNA-PK Inactivated Cells
Frontiers in Oncology, 2022 The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) with a Ku70/Ku80 heterodimer constitutes the intact DNA-PK kinase, which is an upstream component of the DNA repair machinery that signals the DNA damage, orchestrates the DNA repair, and ...Liwei Song, Liwei Song, Mengjun Yu, Renjing Jin, Meng Gu, Ziyu Wang, Dailun Hou, Shaofa Xu, Jinghui Wang, Jinghui Wang, Teng Ma +10 moredoaj +1 more sourceQitanTech Nanopore Long-Read Sequencing Enables Rapid Resolution of Complete Genomes of Multi-Drug Resistant Pathogens
Frontiers in Microbiology, 2022 Advancement of novel sequencing technologies facilitates modern life science and medicine unprecedentedly. Exploring complete genome sequences of bacteria by long-read sequencing technology is significant for microbial genomics research.Kai Peng, Kai Peng, Yi Yin, Yi Yin, Yan Li, Yan Li, Shangshang Qin, Yuan Liu, Yuan Liu, Xiaorong Yang, Zhiqiang Wang, Zhiqiang Wang, Ruichao Li, Ruichao Li +13 moredoaj +1 more sourceFrom cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]
, 2014 Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA Matisoo-Smith, Elizabeth A, Soria Hernanz, David F, Schurr, Theodore G., Stanton, Jo-Ann, Martínez-Cruz, Begoña, White, W. T., GaneshPrasad, Arun Kumar, Lacerda, Daniela R., Matisoo Smith, Elizabeth, White, W. Timothy J., Vilar, Miguel G., Platt, Daniel E., Parida, Laxmi, Hernanz, Soria, Martínez Cruz, Begoña, Kaplan, Matthew E., Clarke, Andrew, Schurr, Theodore G, Matisoo-Smith, Lisa, Matisoo-Smith, Elizabeth, Mitchell, R. John, Matthew E Kaplan, Vieira, Pedro Paulo, Jo-Ann L Stanton, Matisoo-Smith, Elizabeth A., Dulik, Matthew C., Zalloua, Pierre A., Kaplan, Matthew, Tyler-Smith, Chris, Kaplan, M., W Timothy J White, Santhakumari, Arun Varatharajan, Haak, Wolfgang, Quintana-Murci, Lluis, Stefan Prost, null null, Comas, David, Ziegle, Janet S., Swamikrishnan, Pandikumar, Kaplan, Matthew E, Wells, R. Spencer, HASH(0x55a67fb2a340), White, W Timothy J, Cooper, Alan, Clarke, Andrew C, Der Sarkissian, Clio S.I., The, Genographic Consortium, Stanton, J., Bertranpetit, Jaume, 1952-, White, W Timothy J., Stanton, Jo-Ann L., Stanton, Jo Ann L., Pitchappan, Ramasamy, Adhikarla, Syama, Santos, Fabrício R., Comas, David, 1969-, Haber, Marc; id_orcid, Soodyall, Himla, Royyuru, Ajay K., Prost, Stefan, Adler, Christina J., Renfrew, Colin, Jin, Li, Clark, Andrew, Elizabeth A Matisoo-Smith, Andrew C Clarke, Matisoo-Smith, E., Li, Shilin, The Genographic Consortium, Balanovsky, Oleg, Balanovska, Elena, Clarke, Andrew C., Prost, S., Merchant, Nirav C., Andrew C Clarke, Owings, Amanda C., Stanton, Jo-Ann L, White, W., Bertranpetit, Jaume, Gaieski, Jill B., Genographic Consortium, Haber, Marc, 1980-, Genographic Consortium,, Clarke, A. +83 morecore +1 more sourceSpecies identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]
, 2013 The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay +23 morecore +1 more sourceLong-read sequencing data analysis for yeasts [PDF]
Nature Protocols, 2018 Long-read sequencing technologies have become increasingly popular due to their strengths in resolving complex genomic regions. As a leading model organism with small genome size and great biotechnological importance, the budding yeast Saccharomyces cerevisiae has many isolates currently being sequenced with long reads.Yue, Jia-Xing, Liti, Gianniopenaire +3 more sourcesUtility of long-read sequencing for All of Us
Nature Communications, 2023 Abstract The All of Us (AoU) initiative aims to sequence the genomes of over one million Americans from diverse ethnic backgrounds to improve personalized medical care. In a recent technical pilot, we compare the performance of traditional short-read sequencing with long-read sequencing in a small cohort of samples from the HapMap ...M. Mahmoud, Y. Huang, K. Garimella, P. A. Audano, W. Wan, N. Prasad, R. E. Handsaker, S. Hall, A. Pionzio, M. C. Schatz, M. E. Talkowski, E. E. Eichler, S. E. Levy, F. J. Sedlazeck +13 moreopenaire +4 more sourcesLong-read sequence assembly: a technical evaluation in barley [PDF]
The Plant Cell, 2021 AbstractSequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources and often several complementary sequence assembly and genome mapping approaches. The recent development of fast and accurate long-read sequencing by circular consensus sequencing (CCS) on the PacBio platform may greatly ...Mascher, Martin, Wicker, Thomas, Jenkins, Jerry, Plott, Christopher, Lux, Thomas, Koh, Chu Shin, Ens, Jennifer, Gundlach, Heidrun, Boston, Lori B, Tulpová, Zuzana, Holden, Samuel, Hernández-Pinzón, Inmaculada, Scholz, Uwe, Mayer, Klaus F X, Spannagl, Manuel, Pozniak, Curtis J, Sharpe, Andrew G, Šimková, Hana, Moscou, Matthew J, Grimwood, Jane, Schmutz, Jeremy, Stein, Nils +21 moreopenaire +6 more sourcesLong-read sequencing provides uniform coverage across deletion-prone region in the virus genome.
, 2022 Panel A shows the percentage of the virus genome mapped sequencing reads (A) and virus genome fraction covered with Nanopore sequencing data (B), in the positive control (VR1986D ATCC SARS-CoV-2 RNA).Li Chen (5749), Lora V. Hooper (9645168), Jeffrey SoRelle (11944587), Chaoying Liang (9666165), Jinchun Zhou (425820), Brandi Cantarel (3566111), Bo Zhang (6559), Carlos Arana (9666168), Matthew Brock (11510734), Prithvi Raj (6635063) +9 morecore +1 more source