Results 21 to 30 of about 383,643 (254)

DNA read count calibration for single-molecule, long-read sequencing

Scientific Reports, 2022
There are many applications in which quantitative information about DNA mixtures with different molecular lengths is important. Gene therapy vectors are much longer than can be sequenced individually via short-read NGS.
Luis M. M. Soares, Terrence Hanscom, Donald E. Selby, Samuel Adjei, Wei Wang, Dariusz Przybylski, John F. Thompson   +6 more
doaj   +1 more source

Long-read sequencing in human genetics [PDF]

Medizinische Genetik, 2019
Abstract Sanger sequencing revolutionized molecular genetics 40 years ago. However, next-generation sequencing technologies became further game changers and shaped our current view on genome structure and function in health and disease.
Kraft, Florian, Kurth, Ingo
openaire   +2 more sources

Long-read sequencing in fungal identification [PDF]

Microbiology Australia, 2022
Long-read sequencing is currently supported by sequencing platforms from Pacific Biosciences and Oxford Nanopore Technologies, both of which generate ultra-long reads.
Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang   +2 more
doaj  

Are we there yet? Benchmarking low-coverage nanopore long-read sequencing for the assembling of mitochondrial genomes using the vulnerable silky shark Carcharhinus falciformis

BMC Genomics, 2022
Background Whole mitochondrial genomes are quickly becoming markers of choice for the exploration of within-species genealogical and among-species phylogenetic relationships. Most often, ‘primer walking’ or ‘long PCR’ strategies plus Sanger sequencing or
J. Antonio Baeza, F. J. García-De León
doaj   +1 more source

Will long-read sequencing technologies replace short-read sequencing technologies in the next 10 years?

African Journal of Laboratory Medicine, 2020
No abstract available.
Boluwatife A. Adewale
doaj   +1 more source

Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation

BMC Bioinformatics, 2021
Background With the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation (SV).
Tao Jiang, Shiqi Liu, Shuqi Cao, Yadong Liu, Zhe Cui, Yadong Wang, Hongzhe Guo   +6 more
doaj   +1 more source

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, 2022
Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition and function than short-read sequencing.
N. V. Patin, K. D. Goodwin
doaj   +1 more source

Utility of long-read sequencing for All of Us

Nature Communications, 2023
Abstract The All of Us (AoU) initiative aims to sequence the genomes of over one million Americans from diverse ethnic backgrounds to improve personalized medical care. In a recent technical pilot, we compare the performance of traditional short-read sequencing with long-read sequencing in a small cohort of samples from the HapMap ...
M. Mahmoud, Y. Huang, K. Garimella, P. A. Audano, W. Wan, N. Prasad, R. E. Handsaker, S. Hall, A. Pionzio, M. C. Schatz, M. E. Talkowski, E. E. Eichler, S. E. Levy, F. J. Sedlazeck   +13 more
openaire   +4 more sources

Long-read sequence assembly: a technical evaluation in barley [PDF]

The Plant Cell, 2021
AbstractSequence assembly of large and repeat-rich plant genomes has been challenging, requiring substantial computational resources and often several complementary sequence assembly and genome mapping approaches. The recent development of fast and accurate long-read sequencing by circular consensus sequencing (CCS) on the PacBio platform may greatly ...
Mascher, Martin, Wicker, Thomas, Jenkins, Jerry, Plott, Christopher, Lux, Thomas, Koh, Chu Shin, Ens, Jennifer, Gundlach, Heidrun, Boston, Lori B, Tulpová, Zuzana, Holden, Samuel, Hernández-Pinzón, Inmaculada, Scholz, Uwe, Mayer, Klaus F X, Spannagl, Manuel, Pozniak, Curtis J, Sharpe, Andrew G, Šimková, Hana, Moscou, Matthew J, Grimwood, Jane, Schmutz, Jeremy, Stein, Nils   +21 more
openaire   +6 more sources

Long-read sequencing data analysis for yeasts [PDF]

Nature Protocols, 2018
Long-read sequencing technologies have become increasingly popular due to their strengths in resolving complex genomic regions. As a leading model organism with small genome size and great biotechnological importance, the budding yeast Saccharomyces cerevisiae has many isolates currently being sequenced with long reads.
Yue, Jia-Xing, Liti, Gianni
openaire   +3 more sources