Results 21 to 30 of about 7,947,893 (291)

Genome Analysis Methods using Long Read Nanopore Sequencing [PDF]

, 2021
Third-generation long-read technologies denote the latest progression in high throughput DNA and RNA sequence analysis. Complementing the widespread second-generation short-read platforms, long-read sequencing adds unique application opportunities by ...
Giesselmann, Pay, Gießelmann, P. ; https://orcid.org/   +1 more
core   +1 more source

Annotation of Full-Length Long Noncoding RNAs with Capture Long-Read Sequencing (CLS). [PDF]

, 2021
Metazoan genomes produce thousands of long-noncoding RNAs (lncRNAs), of which just a small fraction have been well characterized. Understanding their biological functions requires accurate annotations, or maps of the precise location and structure of ...
Sílvia Carbonell Sala, Guigó Serra, Roderic, Roderic Guigó, Barbara Uszczyńska-Ratajczak, Johnson, Rory, Carbonell Sala, Sílvia, Julien Lagarde, Lagarde, Julien, Rory Johnson, Guigó, Roderic, Uszczyńska-Ratajczak, Barbara   +10 more
core   +1 more source

Long-read sequencing in human genetics [PDF]

Medizinische Genetik, 2019
Abstract Sanger sequencing revolutionized molecular genetics 40 years ago. However, next-generation sequencing technologies became further game changers and shaped our current view on genome structure and function in health and disease.
Kraft, Florian, Kurth, Ingo
openaire   +2 more sources

long-read-tools.org: an interactive catalogue of analysis methods for long-read sequencing data

, 2021
© The Author(s) 2021. Published by Oxford University Press GigaScience. BACKGROUND: The data produced by long-read third-generation sequencers have unique characteristics compared to short-read sequencing data, often requiring tailored analysis tools for
ME Ritchie (9757601), SL Amarasinghe (10493405), Quentin Gouil (9493208)   +2 more
core   +1 more source

Long-read whole genome sequencing of human T cells

, 2023
This dataset represent long read sequencing of single human T cells isolated from a human donor. The data set include Illumina whole genome sequencing of 16 single T cells and PacBio HiFi whole genome sequenicng of 5 single T cells   
Joanna Hård (10518953), Jakob Michaelsson (10154198)   +1 more
core   +1 more source

Comparing genomes recovered from time-series metagenomes using long- and short-read sequencing technologies

Microbiome, 2023
Background Over the past years, sequencing technologies have expanded our ability to examine novel microbial metabolisms and diversity previously obscured by isolation approaches.
Luis H. Orellana, Karen Krüger, Chandni Sidhu, Rudolf Amann   +3 more
doaj   +1 more source

Long-Read Sequencing Improves Recovery of Picoeukaryotic Genomes and Zooplankton Marker Genes from Marine Metagenomes

mSystems, 2022
Long-read sequencing offers the potential to improve metagenome assemblies and provide more robust assessments of microbial community composition and function than short-read sequencing.
N. V. Patin, K. D. Goodwin
doaj   +1 more source

Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation

BMC Bioinformatics, 2021
Background With the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation (SV).
Tao Jiang, Shiqi Liu, Shuqi Cao, Yadong Liu, Zhe Cui, Yadong Wang, Hongzhe Guo   +6 more
doaj   +1 more source

Quantifying single nucleotide variant detection sensitivity in exome sequencing [PDF]

, 2013
BACKGROUND: The targeted capture and sequencing of genomic regions has rapidlydemonstrated its utility in genetic studies. Inherent in this technology isconsiderable heterogeneity of target coverage and this is expected tosystematically impact our ...
Taylor, Martin S.; id_orcid, Andrew P Jackson, Alison M Meynert, Jackson, Andrew, Bicknell, Louise, Martin S Taylor, Matthew E Hurles, Meynert, Alison, Louise S Bicknell   +8 more
core   +1 more source

Migrating to Long-Read Sequencing for Clinical Routine TKI Resistance Mutation Screening

Cancer Informatics, 2022
Objective: The aim of this project was to implement long-read sequencing for BCR-ABL1 TKI resistance mutation screening in a clinical setting for patients undergoing treatment for chronic myeloid leukemia.
Wesley Schaal, Adam Ameur, Ulla Olsson-Strömberg, Monica Hermanson, Lucia Cavelier, Ola Spjuth   +5 more
doaj   +1 more source