Results 21 to 30 of about 672,449 (294)
Long-read sequencing in human genetics [PDF]
Medizinische Genetik, 2019 Abstract Sanger sequencing revolutionized molecular genetics 40 years ago. However, next-generation sequencing technologies became further game changers and shaped our current view on genome structure and function in health and disease.
Kraft, Florian, Kurth, Ingo
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Long-Read Annotation: Automated Eukaryotic Genome Annotation Based on Long-Read cDNA Sequencing [PDF]
Plant Physiology, 2018 Single-molecule full-length complementary DNA (cDNA) sequencing can aid genome annotation by revealing transcript structure and alternative splice forms, yet current annotation pipelines do not incorporate such information. Here we present long-read annotation (LoReAn) software, an automated annotation pipeline utilizing short- and long-read cDNA ...
David E. Cook +5 more
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Discovery and genotyping of structural variation from long-read haploid genome sequence data [PDF]
, 2016 In an effort to more fully understand the full spectrum of human genetic variation, we generated deep single-molecule, real-time (SMRT) sequencing data from two haploid human genomes.
Boitano, Matthew +15 more
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Ultra-accurate microbial amplicon sequencing with synthetic long reads
Microbiome, 2021 Background Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing.
Benjamin J. Callahan +4 more
doaj +1 more source
Frontiers in Microbiology, 2022 Advancement of novel sequencing technologies facilitates modern life science and medicine unprecedentedly. Exploring complete genome sequences of bacteria by long-read sequencing technology is significant for microbial genomics research.
Kai Peng +13 more
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Migrating to Long-Read Sequencing for Clinical Routine TKI Resistance Mutation Screening
Cancer Informatics, 2022 Objective: The aim of this project was to implement long-read sequencing for BCR-ABL1 TKI resistance mutation screening in a clinical setting for patients undergoing treatment for chronic myeloid leukemia.
Wesley Schaal +5 more
doaj +1 more source
DiBELLA: Distributed long read to long read alignment [PDF]
, 2019 We present a parallel algorithm and scalable implementation for genome analysis, specifically the problem of finding overlaps and alignments for data from "third generation" long read sequencers [29]. While long sequences of DNA offer enormous advantages
Buluç, A +4 more
core +2 more sources
Long-read sequencing data analysis for yeasts [PDF]
Nature Protocols, 2018 Long-read sequencing technologies have become increasingly popular due to their strengths in resolving complex genomic regions. As a leading model organism with small genome size and great biotechnological importance, the budding yeast Saccharomyces cerevisiae has many isolates currently being sequenced with long reads.
Yue, Jia-Xing, Liti, Gianni
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Streamlining Quantitative Analysis of Long RNA Sequencing Reads [PDF]
International Journal of Molecular Sciences, 2020 Transcriptome analyses allow for linking RNA expression profiles to cellular pathways and phenotypes. Despite improvements in sequencing methodology, whole transcriptome analyses are still tedious, especially for methodologies producing long reads. Currently, available data analysis software often lacks cost- and time-efficient workflows.
Sebastian Oeck +3 more
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Finished Genome Sequences of Xanthomonas fragariae, the Cause of Bacterial Angular Leaf Spot of Strawberry. [PDF]
, 2016 Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit.
Henry, Peter M, Leveau, Johan HJ
core +2 more sources