Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells [PDF]
BiosensorsFirefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes.
Vanessa R. Bevilaqua +4 more
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Mapping the structural dynamics of red- and blue-emitting beetle luciferases revealed by HDX-MS [PDF]
Life Science AllianceUsing HDX-MS, this study demonstrates that conformational flexibility in beetle luciferases governs bioluminescence color, revealing how active site dynamics and domain-specific mutations fine-tune red- and blue-shifted light emission. Beetle luciferases
Abdul-Rahman Kharbatli +6 more
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Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]
SensorsCoelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim +3 more
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CadmiLume: A Novel Smartphone-Based Bioluminescence Color-Tuning Assay and Biosensor for Cadmium and Heavy Metal Detection in Water Samples [PDF]
Methods and ProtocolsHeavy metal contamination of soil and water is a growing environmental concern, especially mercury, lead, and cadmium. Therefore, fast and reliable methodologies to assess contamination in the field are in demand.
Vadim R. Viviani +2 more
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Applications of bioluminescence-mediated photodynamic therapy (BL-PDT) for the treatment of deep-seated tumours [PDF]
Discover OncologyBioluminescence, the light generated through biochemical reactions involving luciferases in living organisms, has been the focus of extensive research due to its diverse applications.
Mpho Mohlongo +2 more
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Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays [PDF]
Frontiers in ChemistryBioluminescent high-throughput screening (HTS) assays, based largely on the activity of firefly (FLuc), Renilla (RLuc), and/or NanoLuc (NLuc) luciferases, are widely utilised in research and drug discovery. In this study, we quantify the luciferase-based
Francesca Canyelles i Font +4 more
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An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging [PDF]
Scientific ReportsBioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao +9 more
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Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2
陆军军医大学学报, 2023 Objective To construct a new fusion luciferase-labeled Staphylococcus aureus (S. aureus) tracing system. Methods An S. aureus tracer strain was constructed by using DNA homologous recombination technique.
HU Zhen, SHANG Weilong, RAO Xiancai
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Substrate cooperativity in marine luciferases. [PDF]
PLoS ONE, 2012 Marine luciferases are increasingly used as reporters to study gene regulation. These luciferases have utility in bioluminescent assay development, although little has been reported on their catalytic properties in response to substrate concentration ...
George Tzertzinis +2 more
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A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence [PDF]
Open Biology, 2017 Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes.
Jérôme Delroisse +6 more
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