A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b.
Nick Van Reet +4 more
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Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells [PDF]
BiosensorsFirefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes.
Vanessa R. Bevilaqua +4 more
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Coelenterazine Analogs for Bioassays and Molecular Imaging [PDF]
SensorsCoelenterazine (CTZ) is a common substrate of marine luciferases upon emission of bioluminescence (BL) in living organisms. Because CTZ works as a “luminophore” in the process of BL emission, the chemical modification has been centered for improving the ...
Sung-Bae Kim +3 more
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CadmiLume: A Novel Smartphone-Based Bioluminescence Color-Tuning Assay and Biosensor for Cadmium and Heavy Metal Detection in Water Samples [PDF]
Methods and ProtocolsHeavy metal contamination of soil and water is a growing environmental concern, especially mercury, lead, and cadmium. Therefore, fast and reliable methodologies to assess contamination in the field are in demand.
Vadim R. Viviani +2 more
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Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays [PDF]
Sensors, 2023 In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim +3 more
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Applications of bioluminescence-mediated photodynamic therapy (BL-PDT) for the treatment of deep-seated tumours [PDF]
Discover OncologyBioluminescence, the light generated through biochemical reactions involving luciferases in living organisms, has been the focus of extensive research due to its diverse applications.
Mpho Mohlongo +2 more
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Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays [PDF]
Frontiers in ChemistryBioluminescent high-throughput screening (HTS) assays, based largely on the activity of firefly (FLuc), Renilla (RLuc), and/or NanoLuc (NLuc) luciferases, are widely utilised in research and drug discovery. In this study, we quantify the luciferase-based
Francesca Canyelles i Font +4 more
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An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging [PDF]
Scientific ReportsBioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao +9 more
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ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21 [PDF]
BiologyThe L. fermentum U-21 strain, known for secreting chaperones into the extracellular milieu, emerges as a promising candidate for the development of novel therapeutics termed disaggregases for Parkinson’s disease.
Rahaf N. Al Ebrahim +8 more
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Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2
陆军军医大学学报, 2023 Objective To construct a new fusion luciferase-labeled Staphylococcus aureus (S. aureus) tracing system. Methods An S. aureus tracer strain was constructed by using DNA homologous recombination technique.
HU Zhen, SHANG Weilong, RAO Xiancai
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