Results 11 to 20 of about 14,115 (284)
Biosensors, 2022 Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades.
Vadim R. Viviani +2 more
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Approaches to engineer stability of beetle luciferases
Computational and Structural Biotechnology Journal, 2012 Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas.
Mikhail Koksharov, Natalia Ugarova
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Leaving the Dark Side? Insights Into the Evolution of Luciferases
Frontiers in Marine Science, 2021 Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, a luciferin substrate, an oxygen derivative, and a specialised luciferase enzyme.
Jérôme Delroisse +3 more
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Luciferase‐free Luciferin Electrochemiluminescence
Angewandte Chemie International Edition, 2022 AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti +7 more
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Luciferase Controlled Protein Interactions
Journal of the American Chemical Society, 2021 Protein trafficking and protein-protein interactions (PPI) are central to regulatory processes in cells. Induced dimerization systems have been developed to control PPI and regulate protein trafficking (localization) or interactions. Chemically induced dimerization (CID) has proven to be a robust approach to control protein interactions and ...
Dalu Chang +4 more
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Expanding luciferase reporter systems for cell-free protein expression
Scientific Reports, 2022 Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-translation (TXTL) systems, for research and practical applications such as engineering genetic parts, validating genetic circuits, and biosensor outputs. Currently,
Wakana Sato +4 more
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Life, 2023 Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova +3 more
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Multiplex quadruple bioluminescent assay system
Scientific Reports, 2022 Bioluminescence (BL) is unique cold body radiation of light, generated by luciferin–luciferase reactions and commonly used in various bioassays and molecular imaging.
Genta Kamiya +3 more
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Near-infrared bioluminescence imaging of two cell populations in living mice
STAR Protocols, 2021 Summary: Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model.
Giorgia Zambito, Laura Mezzanotte
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Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]
PLoS ONE, 2014 Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
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