Results 11 to 20 of about 19,699 (283)
Optimisation of bioluminescent reporters for use with mycobacteria. [PDF]
PLoS ONE, 2010 BackgroundMycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology ...
Nuria Andreu +10 more
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Biosensors, 2022 Firefly luciferases catalyze the efficient production of yellow-green light under normal physiological conditions, having been extensively used for bioanalytical purposes for over 5 decades.
Vadim R. Viviani +2 more
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Substrate cooperativity in marine luciferases. [PDF]
PLoS ONE, 2012 Marine luciferases are increasingly used as reporters to study gene regulation. These luciferases have utility in bioluminescent assay development, although little has been reported on their catalytic properties in response to substrate concentration ...
George Tzertzinis +2 more
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A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence [PDF]
Open Biology, 2017 Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes.
Jérôme Delroisse +6 more
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Approaches to engineer stability of beetle luciferases
Computational and Structural Biotechnology Journal, 2012 Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas.
Mikhail Koksharov, Natalia Ugarova
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Leaving the Dark Side? Insights Into the Evolution of Luciferases
Frontiers in Marine Science, 2021 Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, a luciferin substrate, an oxygen derivative, and a specialised luciferase enzyme.
Jérôme Delroisse +3 more
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Expanding luciferase reporter systems for cell-free protein expression
Scientific Reports, 2022 Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-translation (TXTL) systems, for research and practical applications such as engineering genetic parts, validating genetic circuits, and biosensor outputs. Currently,
Wakana Sato +4 more
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Life, 2023 Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova +3 more
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Multiplex quadruple bioluminescent assay system
Scientific Reports, 2022 Bioluminescence (BL) is unique cold body radiation of light, generated by luciferin–luciferase reactions and commonly used in various bioassays and molecular imaging.
Genta Kamiya +3 more
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Near-infrared bioluminescence imaging of two cell populations in living mice
STAR Protocols, 2021 Summary: Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model.
Giorgia Zambito, Laura Mezzanotte
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