Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]
Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
doaj +1 more source
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye +7 more
doaj +2 more sources
Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens. [PDF]
A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations.
Stekel, Dov J, Welham, Patricia A
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Reversible low-light induced photoswitching of crowned spiropyran-DO3A complexed with gadolinium(III) ions. [PDF]
Photoswitchable spiropyran has been conjugated to the crowned ring system DO3A, which improves its solubility in dipolar and polar media and stabilizes the merocyanine isomer. Adding the lanthanide ion gadolinium(III) to the macrocyclic ring system leads
Brueggemann, Chantal +6 more
core +4 more sources
Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. [PDF]
Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises ...
Aydin, M +3 more
core +1 more source
Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh +5 more
doaj +1 more source
Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]
BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte +5 more
doaj +1 more source
Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]
Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
Beznoskova, Petra +4 more
core +1 more source
A higher spectral range of beetle bioluminescence with infraluciferin
Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul +4 more
doaj +1 more source
Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging. [PDF]
Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate.
Coste, A.T., Dorsaz, S., Sanglard, D.
core +6 more sources

