Results 21 to 30 of about 19,699 (283)

Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]

open access: yesPLoS ONE, 2014
Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
doaj   +1 more source

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate

open access: yesPLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye   +7 more
doaj   +2 more sources

Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens. [PDF]

open access: yes, 2009
A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations.
Stekel, Dov J, Welham, Patricia A
core   +1 more source

Reversible low-light induced photoswitching of crowned spiropyran-DO3A complexed with gadolinium(III) ions. [PDF]

open access: yes, 2012
Photoswitchable spiropyran has been conjugated to the crowned ring system DO3A, which improves its solubility in dipolar and polar media and stabilizes the merocyanine isomer. Adding the lanthanide ion gadolinium(III) to the macrocyclic ring system leads
Brueggemann, Chantal   +6 more
core   +4 more sources

Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. [PDF]

open access: yes, 2014
Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises ...
Aydin, M   +3 more
core   +1 more source

Kinetics of the thermal inactivation and the refolding of bacterial luciferases in Bacillus subtilis and in Escherichia coli differ.

open access: yesPLoS ONE, 2019
Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh   +5 more
doaj   +1 more source

Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]

open access: yesPLoS ONE, 2011
BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte   +5 more
doaj   +1 more source

Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]

open access: yes, 2015
Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
Beznoskova, Petra   +4 more
core   +1 more source

A higher spectral range of beetle bioluminescence with infraluciferin

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul   +4 more
doaj   +1 more source

Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging. [PDF]

open access: yes, 2017
Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate.
Coste, A.T., Dorsaz, S., Sanglard, D.
core   +6 more sources

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