Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays
Sensors, 2023 In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim +3 more
doaj +1 more source
Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens. [PDF]
, 2009 A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations.
Stekel, Dov J, Welham, Patricia A
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Reversible low-light induced photoswitching of crowned spiropyran-DO3A complexed with gadolinium(III) ions. [PDF]
, 2012 Photoswitchable spiropyran has been conjugated to the crowned ring system DO3A, which improves its solubility in dipolar and polar media and stabilizes the merocyanine isomer. Adding the lanthanide ion gadolinium(III) to the macrocyclic ring system leads
Brueggemann, Chantal +6 more
core +4 more sources
PLoS ONE, 2022 Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye +7 more
doaj +2 more sources
Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. [PDF]
, 2014 Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises ...
Aydin, M +3 more
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Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin [PDF]
, 2006 Epithelial tight junctions participate in the regulation of gene expression by controlling the activity of transcription factors that can interact with junctional components.
Balda, MS +6 more
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Red-Shifted Firefly Luciferase Optimized for Candida albicans In vivo Bioluminescence Imaging. [PDF]
, 2017 Candida albicans is a major fungal pathogen causing life-threatening diseases in immuno-compromised patients. The efficacy of current drugs to combat C. albicans infections is limited, as these infections have a 40-60% mortality rate.
Coste, A.T., Dorsaz, S., Sanglard, D.
core +6 more sources
Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]
, 2015 Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
Beznoskova, Petra +4 more
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PLoS ONE, 2019 Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh +5 more
doaj +1 more source
Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes [PDF]
, 2013 Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Badr, Christian E +8 more
core +2 more sources